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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 1 (pfkA1)
  4. Fructose-bisphosphate aldolase class 1 (fda), Fructose-bisphosphate aldolase class 2 (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenUniRule annotation1
Metal bindingi121Magnesium; catalyticUniRule annotation1
Sitei122Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei146Proton acceptorUniRule annotation1
Binding sitei181Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei246SubstrateUniRule annotation1
Binding sitei291Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 92ATPUniRule annotation2
Nucleotide bindingi120 – 123ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Synonyms:pfkA
Ordered Locus Names:sll0745
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119991 – 384ATP-dependent 6-phosphofructokinase 2Add BLAST384

Proteomic databases

PRIDEiQ55988.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ55988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 146Substrate bindingUniRule annotation3
Regioni188 – 190Substrate bindingUniRule annotation3
Regioni297 – 300Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248869.
InParanoidiQ55988.
KOiK00850.
OMAiHERVMIV.
PhylomeDBiQ55988.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTKRIGILT SGGDCPGLNA VIRAVVKASA LKGWEVYGIP YGTDGFVEVA
60 70 80 90 100
HGKYQAEDLL LTKHGYALPG MLKGLDVLQF LSGSVLGSLS KGHPEQPAIA
110 120 130 140 150
EAILKGYAIL DLEALIVIGG DGSLDIIYDL AQKGNWHIIA IPKTIDNDVP
160 170 180 190 200
FTDLAVGFST AVDIVTQALY DLTFTAASHE RIIIVQVMGR DAGHLTLHAG
210 220 230 240 250
IAGGADIILI PEITPCLTSE IIRNCCYQLM NLRKSGRHFA LIVISEGVHD
260 270 280 290 300
QNNKKNKHIA DYLAEEISET SQHLCDIKDP AFCDLISLDI RATTLGHLQR
310 320 330 340 350
SGTPLSFDRL LATVFGIRAV ELIEQEIYDQ VVIWRSGKVE HADLKPIISI
360 370 380
IKECHQENRC PFPVDRDGFM VKTAKSLGIY LGED
Length:384
Mass (Da):41,759
Last modified:November 1, 1996 - v1
Checksum:i03034C863DBD9133
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10770.1.
PIRiS77078.

Genome annotation databases

EnsemblBacteriaiBAA10770; BAA10770; BAA10770.
KEGGisyn:sll0745.
PATRICi23843144. VBISynSp132158_3110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10770.1.
PIRiS77078.

3D structure databases

ProteinModelPortaliQ55988.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ55988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10770; BAA10770; BAA10770.
KEGGisyn:sll0745.
PATRICi23843144. VBISynSp132158_3110.

Phylogenomic databases

HOGENOMiHOG000248869.
InParanoidiQ55988.
KOiK00850.
OMAiHERVMIV.
PhylomeDBiQ55988.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA2_SYNY3
AccessioniPrimary (citable) accession number: Q55988
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.