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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
Metal bindingi121 – 1211Magnesium; catalyticUniRule annotation
Sitei122 – 1221Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei146 – 1461Proton acceptorUniRule annotation
Binding sitei181 – 1811Substrate; shared with dimeric partnerUniRule annotation
Binding sitei246 – 2461SubstrateUniRule annotation
Binding sitei291 – 2911Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi91 – 922ATPUniRule annotation
Nucleotide bindingi120 – 1234ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Synonyms:pfkA
Ordered Locus Names:sll0745
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384ATP-dependent 6-phosphofructokinase 2PRO_0000111999Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi1148.sll0745.

Structurei

3D structure databases

ProteinModelPortaliQ55988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1463Substrate bindingUniRule annotation
Regioni188 – 1903Substrate bindingUniRule annotation
Regioni297 – 3004Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiQ55988.
KOiK00850.
OMAiCPGLNAI.
OrthoDBiEOG644ZRM.
PhylomeDBiQ55988.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTKRIGILT SGGDCPGLNA VIRAVVKASA LKGWEVYGIP YGTDGFVEVA
60 70 80 90 100
HGKYQAEDLL LTKHGYALPG MLKGLDVLQF LSGSVLGSLS KGHPEQPAIA
110 120 130 140 150
EAILKGYAIL DLEALIVIGG DGSLDIIYDL AQKGNWHIIA IPKTIDNDVP
160 170 180 190 200
FTDLAVGFST AVDIVTQALY DLTFTAASHE RIIIVQVMGR DAGHLTLHAG
210 220 230 240 250
IAGGADIILI PEITPCLTSE IIRNCCYQLM NLRKSGRHFA LIVISEGVHD
260 270 280 290 300
QNNKKNKHIA DYLAEEISET SQHLCDIKDP AFCDLISLDI RATTLGHLQR
310 320 330 340 350
SGTPLSFDRL LATVFGIRAV ELIEQEIYDQ VVIWRSGKVE HADLKPIISI
360 370 380
IKECHQENRC PFPVDRDGFM VKTAKSLGIY LGED
Length:384
Mass (Da):41,759
Last modified:November 1, 1996 - v1
Checksum:i03034C863DBD9133
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10770.1.
PIRiS77078.
RefSeqiNP_442699.1. NC_000911.1.
YP_005652760.1. NC_017277.1.
YP_007452575.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10770; BAA10770; BAA10770.
GeneIDi952855.
KEGGisyn:sll0745.
PATRICi23843144. VBISynSp132158_3110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10770.1.
PIRiS77078.
RefSeqiNP_442699.1. NC_000911.1.
YP_005652760.1. NC_017277.1.
YP_007452575.1. NC_020286.1.

3D structure databases

ProteinModelPortaliQ55988.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1148.sll0745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10770; BAA10770; BAA10770.
GeneIDi952855.
KEGGisyn:sll0745.
PATRICi23843144. VBISynSp132158_3110.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiQ55988.
KOiK00850.
OMAiCPGLNAI.
OrthoDBiEOG644ZRM.
PhylomeDBiQ55988.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiPFKA2_SYNY3
AccessioniPrimary (citable) accession number: Q55988
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.