Q55988 (K6PF2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 2
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 2
Phosphohexokinase 2

Gene names

Name:	pfkA2
Synonyms:	pfkA
Ordered Locus Names:	sll0745

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	6-phosphofructokinase 2 HAMAP-Rule MF_00339		PRO_0000111999
Regions
	Nucleotide binding	23 – 27	5	ATP By similarity
	Nucleotide binding	173 – 177	5	ATP By similarity
	Nucleotide binding	190 – 206	17	ATP By similarity
Sites
	Active site	146	1	Proton acceptor By similarity
	Metal binding	204	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55988 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 03034C863DBD9133	FASTA	384	41,759
	10 20 30 40 50 60 MGTKRIGILT SGGDCPGLNA VIRAVVKASA LKGWEVYGIP YGTDGFVEVA HGKYQAEDLL 70 80 90 100 110 120 LTKHGYALPG MLKGLDVLQF LSGSVLGSLS KGHPEQPAIA EAILKGYAIL DLEALIVIGG 130 140 150 160 170 180 DGSLDIIYDL AQKGNWHIIA IPKTIDNDVP FTDLAVGFST AVDIVTQALY DLTFTAASHE 190 200 210 220 230 240 RIIIVQVMGR DAGHLTLHAG IAGGADIILI PEITPCLTSE IIRNCCYQLM NLRKSGRHFA 250 260 270 280 290 300 LIVISEGVHD QNNKKNKHIA DYLAEEISET SQHLCDIKDP AFCDLISLDI RATTLGHLQR 310 320 330 340 350 360 SGTPLSFDRL LATVFGIRAV ELIEQEIYDQ VVIWRSGKVE HADLKPIISI IKECHQENRC 370 380 PFPVDRDGFM VKTAKSLGIY LGED

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10770.1.
PIR	S77078.
RefSeq	NP_442699.1. NC_000911.1. YP_005652760.1. NC_017277.1. YP_007452575.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55988.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.sll0745.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10770; BAA10770; BAA10770.
GeneID	12254954. 14618257. 952855.
KEGG	syn:sll0745. syy:SYNGTS_2807.
PATRIC	23843144. VBISynSp132158_3110.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248869.
KO	K00850.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_SYNY3

Accession

Primary (citable) accession number: Q55988

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program