Q55978 (QUEF_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	slr0711

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000163013

Sequences

	Sequence		Length	Mass (Da)
	Q55978 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BC1B6B9AA42818C4	FASTA	137	15,940
	10 20 30 40 50 60 MTETTITTSD NVEKYGEREI RDAQLITFPN PRPGRRYDVH ITLPEFTCKC PFSGYPDFAT 70 80 90 100 110 120 LYLTYCPDQK VVELKSIKLY INSYRDRHIP HEEVTNQILD DFVAVANPLY ARLKADFNPR 130 GNVHTVIEVE YHQEKAS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10757.1.
PIR	S77065.
RefSeq	NP_442686.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55978.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55978.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	954928.
GenomeReviews	Gene locus slr0711 in contig BA000022_GR.
KEGG	syn:slr0711.
NMPDR	fig|1148.1.peg.2787.
PATRIC	23843118. VBISynSp132158_3097.
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	FRNAGIF.
PhylomeDB	Q55978.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0711-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_SYNY3

Accession

Primary (citable) accession number: Q55978

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program