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Protein

DNA polymerase I

Gene

polA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Ordered Locus Names:slr0707
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 986986DNA polymerase IPRO_0000101255Add
BLAST

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.By similarity

Protein-protein interaction databases

IntActiQ55971. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ55971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 3033035'-3' exonucleaseAdd
BLAST
Domaini304 – 5922893'-5' exonucleaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni593 – 986394PolymeraseAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 5'-3' exonuclease domain.Curated

Phylogenomic databases

HOGENOMiHOG000020999.
InParanoidiQ55971.
KOiK02335.
OMAiCFDTETT.
OrthoDBiEOG6SJJH7.
PhylomeDBiQ55971.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFMSAKSPLL LLVDGHSLAF RAYYAFGLSK KGPLRTTAGI PTSVCFGFLN
60 70 80 90 100
SLMQVMESQK PAAIAIAFDR REPTFRHEAD GAYKSNRQET PEDFAEDLSY
110 120 130 140 150
LQQLLEALNL QTITYAGYEA DDILGTLACQ GSDAGYQVKI LSGDRDLFQL
160 170 180 190 200
VSPEKNISVL YLTRNPFSSN TGYDELDWQG VVDKMGVTPA QIVDFKALCG
210 220 230 240 250
DKSDCIPGIN GIGEKTAIKL LAEYETLEKV YENLAQIKGA LKTRLDNGKD
260 270 280 290 300
DAMHSQMLAR IVVDVPLPVT WEDLQLTGFS TDRLVPLLEK LELRTFIDKI
310 320 330 340 350
QAFHRNFSDN QSPVPMGNEA DNGEPKKTVK AKKSKEKVNP DDSQQLSLFD
360 370 380 390 400
GVPVVNQEDG LITIQLPKQI QPQIITTIAQ LEALVEELKK HTDADFPVAW
410 420 430 440 450
DTETDSLDPL VANLVGIGCA WGQEPNQVAY IPLKHHQGEQ LSLGIIKDLL
460 470 480 490 500
GEILGNAIYP KVLQNAKFDR RVLAHHGIEL GGVVLDTMLA SYVLQPEETH
510 520 530 540 550
NLTDLCRRYN LGLVALSYKD LGLKKDQTIA DLPLETAGQY CGLDCYATYL
560 570 580 590 600
LASKLQKELD QYPELKEILK EIEQPLEKIL AAMEDRGIRI DCDYLQTLSQ
610 620 630 640 650
QLAENLLTIE TAAYEAAGES FNLSSPKQLG TILFDKLGLD RKKSRKTKTG
660 670 680 690 700
YSTDHATLEK LQGDHPIIDA ILEHRTLAKL KSTYVDALPE LVNGQTQRIH
710 720 730 740 750
TDFNQAVTST GRLSSSNPNL QNIPIRSDFS RQIRRAFLPQ KDWLLVSADY
760 770 780 790 800
SQIELRILAH LSQEPVLLQA YGDRQDVHGV TAKLLFGKED ITPAERNLGK
810 820 830 840 850
TINFGVIYGM GAQRFARETG ISAVEGREFI DRYHRTYAQV FDYLETMKLE
860 870 880 890 900
AIAKGYVTTI VGRRRYFNFV TEALRQLRGK TVTELDLVDV KMNYNDAQLL
910 920 930 940 950
RSAANAPIQG SSADIIKIAM VKLAKLLESY QTRMLLQVHD ELIFEMPPEE
960 970 980
WEELAPLIQN TMEQALTLSV PLVVEMHRGS NWMEAK
Length:986
Mass (Da):110,287
Last modified:November 1, 1996 - v1
Checksum:iC87852AD07487FB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10748.1.
PIRiS77056.

Genome annotation databases

EnsemblBacteriaiBAA10748; BAA10748; BAA10748.
KEGGisyn:slr0707.
PATRICi23843100. VBISynSp132158_3088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10748.1.
PIRiS77056.

3D structure databases

ProteinModelPortaliQ55971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55971. 3 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10748; BAA10748; BAA10748.
KEGGisyn:slr0707.
PATRICi23843100. VBISynSp132158_3088.

Phylogenomic databases

HOGENOMiHOG000020999.
InParanoidiQ55971.
KOiK02335.
OMAiCFDTETT.
OrthoDBiEOG6SJJH7.
PhylomeDBiQ55971.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiDPO1_SYNY3
AccessioniPrimary (citable) accession number: Q55971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.