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Protein

Transcriptional repressor SmtB homolog

Gene

ziaR

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor of the expression of the ziaA gene. Controls zinc homeostasis by triggering ZiaA-mediated efflux of excess zinc into the periplasm.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201ZincPROSITE-ProRule annotation
Metal bindingi26 – 261ZincPROSITE-ProRule annotation
Metal bindingi71 – 711ZincPROSITE-ProRule annotation
Metal bindingi73 – 731ZincPROSITE-ProRule annotation
Metal bindingi114 – 1141ZincPROSITE-ProRule annotation
Metal bindingi116 – 1161ZincPROSITE-ProRule annotation
Metal bindingi127 – 1271ZincPROSITE-ProRule annotation
Metal bindingi130 – 1301ZincPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi72 – 9120H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor SmtB homolog
Gene namesi
Name:ziaR
Synonyms:smtB
Ordered Locus Names:sll0792
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711C → S: Loss of ability to respond to zinc. Still capable of binding DNA; when associated with S-73. 1 Publication
Mutagenesisi73 – 731C → S: Loss of ability to respond to zinc. Still capable of binding DNA; when associated with S-71. 1 Publication
Mutagenesisi116 – 1161H → R: Loss of ability to respond to zinc. Still capable of binding DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 132132Transcriptional repressor SmtB homologPRO_0000160627Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ55940.
SMRiQ55940. Positions 29-130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 13295HTH arsR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH arsR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0640.
HOGENOMiHOG000144506.
InParanoidiQ55940.
OMAiCDIANIV.
OrthoDBiEOG6Z3KS4.
PhylomeDBiQ55940.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR018334. ArsR_HTH.
IPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
PROSITEiPS00846. HTH_ARSR_1. 1 hit.
PS50987. HTH_ARSR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSSLSKSQ SCQNEEMPLC DQPLVHLEQV RQVQPEVMSL DQAQQMAEFF
60 70 80 90 100
SALADPSRLR LMSALARQEL CVCDLAAAMK VSESAVSHQL RILRSQRLVK
110 120 130
YRRVGRNVYY SLADNHVMNL YREVADHLQE SD
Length:132
Mass (Da):15,083
Last modified:November 1, 1996 - v1
Checksum:i08A7B19849B186C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10706.1.
PIRiS77014.

Genome annotation databases

EnsemblBacteriaiBAA10706; BAA10706; BAA10706.
KEGGisyy:SYNGTS_2743.
syz:MYO_127690.
PATRICi23843014. VBISynSp132158_3045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10706.1.
PIRiS77014.

3D structure databases

ProteinModelPortaliQ55940.
SMRiQ55940. Positions 29-130.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10706; BAA10706; BAA10706.
KEGGisyy:SYNGTS_2743.
syz:MYO_127690.
PATRICi23843014. VBISynSp132158_3045.

Phylogenomic databases

eggNOGiCOG0640.
HOGENOMiHOG000144506.
InParanoidiQ55940.
OMAiCDIANIV.
OrthoDBiEOG6Z3KS4.
PhylomeDBiQ55940.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR018334. ArsR_HTH.
IPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
PROSITEiPS00846. HTH_ARSR_1. 1 hit.
PS50987. HTH_ARSR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter."
    Thelwell C., Robinson N.J., Turner-Cavet J.S.
    Proc. Natl. Acad. Sci. U.S.A. 95:10728-10733(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-71; CYS-73 AND HIS-116.
  4. "The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance."
    Busenlehner L.S., Pennella M.A., Giedroc D.P.
    FEMS Microbiol. Rev. 27:131-143(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiZIAR_SYNY3
AccessioniPrimary (citable) accession number: Q55940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.