Q55940 (ZIAR_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor SmtB homolog

Gene names

Name:	ziaR
Synonyms:	smtB
Ordered Locus Names:	sll0792

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor of the expression of the ziaA gene. Controls zinc homeostasis by triggering ZiaA-mediated efflux of excess zinc into the periplasm.
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 HTH arsR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Transcriptional repressor SmtB homolog		PRO_0000160627
Regions
	Domain	38 – 132	95	HTH arsR-type
	DNA binding	72 – 91	20	H-T-H motif Potential
Sites
	Metal binding	20	1	Zinc Potential
	Metal binding	26	1	Zinc Potential
	Metal binding	71	1	Zinc Potential
	Metal binding	73	1	Zinc Potential
	Metal binding	114	1	Zinc Potential
	Metal binding	116	1	Zinc Potential
	Metal binding	127	1	Zinc Potential
	Metal binding	130	1	Zinc Potential
Experimental info
	Mutagenesis	71	1	C → S: Loss of ability to respond to zinc. Still capable of binding DNA; when associated with S-73. Ref.3
	Mutagenesis	73	1	C → S: Loss of ability to respond to zinc. Still capable of binding DNA; when associated with S-71. Ref.3
	Mutagenesis	116	1	H → R: Loss of ability to respond to zinc. Still capable of binding DNA. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q55940 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 08A7B19849B186C9	FASTA	132	15,083
	10 20 30 40 50 60 MSKSSLSKSQ SCQNEEMPLC DQPLVHLEQV RQVQPEVMSL DQAQQMAEFF SALADPSRLR 70 80 90 100 110 120 LMSALARQEL CVCDLAAAMK VSESAVSHQL RILRSQRLVK YRRVGRNVYY SLADNHVMNL 130 YREVADHLQE SD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[3]	"An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter." Thelwell C., Robinson N.J., Turner-Cavet J.S. Proc. Natl. Acad. Sci. U.S.A. 95:10728-10733(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-71; CYS-73 AND HIS-116.
[4]	"The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance." Busenlehner L.S., Pennella M.A., Giedroc D.P. FEMS Microbiol. Rev. 27:131-143(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10706.1.
PIR	S77014.
RefSeq	NP_442635.1. NC_000911.1. YP_005652696.1. NC_017277.1. YP_007452511.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55940.
SMR	Q55940. Positions 29-130.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.sll0792.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10706; BAA10706; BAA10706.
GeneID	12254810. 14618193. 952462.
KEGG	syn:sll0792. syy:SYNGTS_2743.
PATRIC	23843014. VBISynSp132158_3045.
Phylogenomic databases
eggNOG	COG0640.
HOGENOM	HOG000144506.
OMA	CHENNEN.
ProtClustDB	CLSK492657.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR018334. ArsR_HTH. IPR001845. HTH_ArsR_DNA-bd_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01022. HTH_5. 1 hit. [Graphical view]
PRINTS	PR00778. HTHARSR.
SMART	SM00418. HTH_ARSR. 1 hit. [Graphical view]
PROSITE	PS00846. HTH_ARSR_1. 1 hit. PS50987. HTH_ARSR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZIAR_SYNY3

Accession

Primary (citable) accession number: Q55940

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program