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Protein

Bifunctional NMN adenylyltransferase/Nudix hydrolase

Gene

slr0787

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The Nudix hydrolase domain is active on ADP-ribose, (2')-phospho-ADP-ribose, IDP-ribose and NADPH.

Catalytic activityi

ATP + nicotinamide ribonucleotide = diphosphate + NAD+.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional NMN adenylyltransferase/Nudix hydrolase (slr0787)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8321.
BRENDAi2.7.7.1. 382.
3.6.1.13. 382.
UniPathwayiUPA00253; UER00600.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NMN adenylyltransferase/Nudix hydrolase
Including the following 2 domains:
Nicotinamide-nucleotide adenylyltransferase (EC:2.7.7.1)
Alternative name(s):
NAD(+) diphosphorylase
NAD(+) pyrophosphorylase
NMN adenylyltransferase
ADP compounds hydrolase (EC:3.6.1.-)
Gene namesi
Ordered Locus Names:slr0787
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001350111 – 339Bifunctional NMN adenylyltransferase/Nudix hydrolaseAdd BLAST339

Interactioni

Protein-protein interaction databases

DIPiDIP-29582N.
IntActiQ55928. 3 interactors.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 12Combined sources11
Helixi19 – 31Combined sources13
Beta strandi32 – 42Combined sources11
Beta strandi48 – 50Combined sources3
Helixi54 – 62Combined sources9
Helixi67 – 70Combined sources4
Beta strandi73 – 79Combined sources7
Helixi85 – 100Combined sources16
Beta strandi106 – 110Combined sources5
Helixi115 – 121Combined sources7
Beta strandi126 – 130Combined sources5
Helixi139 – 148Combined sources10
Helixi151 – 153Combined sources3
Turni154 – 157Combined sources4
Helixi160 – 169Combined sources10
Helixi173 – 190Combined sources18
Turni191 – 194Combined sources4
Beta strandi195 – 197Combined sources3
Beta strandi201 – 210Combined sources10
Beta strandi213 – 218Combined sources6
Beta strandi221 – 224Combined sources4
Beta strandi231 – 234Combined sources4
Helixi241 – 253Combined sources13
Helixi259 – 264Combined sources6
Beta strandi266 – 272Combined sources7
Beta strandi283 – 291Combined sources9
Beta strandi294 – 296Combined sources3
Beta strandi305 – 307Combined sources3
Beta strandi309 – 313Combined sources5
Helixi314 – 319Combined sources6
Helixi321 – 323Combined sources3
Helixi328 – 335Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QJOX-ray2.60A/B/C1-339[»]
ProteinModelPortaliQ55928.
SMRiQ55928.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ55928.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 335Nudix hydrolasePROSITE-ProRule annotationAdd BLAST137

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 183NMN adenylyltransferaseAdd BLAST183

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi233 – 254Nudix boxAdd BLAST22

Sequence similaritiesi

In the N-terminal section; belongs to the archaeal NMN adenylyltransferase family.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ55928.
KOiK13522.
OMAiYPPVFVT.
PhylomeDBiQ55928.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTKYQYGIY IGRFQPFHLG HLRTLNLALE KAEQVIIILG SHRVAADTRN
60 70 80 90 100
PWRSPERMAM IEACLSPQIL KRVHFLTVRD WLYSDNLWLA AVQQQVLKIT
110 120 130 140 150
GGSNSVVVLG HRKDASSYYL NLFPQWDYLE TGHYPDFSST AIRGAYFEGK
160 170 180 190 200
EGDYLDKVPP AIADYLQTFQ KSERYIALCD EYQFLQAYKQ AWATAPYAPT
210 220 230 240 250
FITTDAVVVQ AGHVLMVRRQ AKPGLGLIAL PGGFIKQNET LVEGMLRELK
260 270 280 290 300
EETRLKVPLP VLRGSIVDSH VFDAPGRSLR GRTITHAYFI QLPGGELPAV
310 320 330
KGGDDAQKAW WMSLADLYAQ EEQIYEDHFQ IIQHFVSKV
Length:339
Mass (Da):38,532
Last modified:November 1, 1996 - v1
Checksum:iB12938EBE97465BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10693.1.
PIRiS77001.

Genome annotation databases

EnsemblBacteriaiBAA10693; BAA10693; BAA10693.
KEGGisyn:slr0787.
PATRICi23842984. VBISynSp132158_3030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10693.1.
PIRiS77001.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QJOX-ray2.60A/B/C1-339[»]
ProteinModelPortaliQ55928.
SMRiQ55928.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29582N.
IntActiQ55928. 3 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10693; BAA10693; BAA10693.
KEGGisyn:slr0787.
PATRICi23842984. VBISynSp132158_3030.

Phylogenomic databases

InParanoidiQ55928.
KOiK13522.
OMAiYPPVFVT.
PhylomeDBiQ55928.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00600.
BioCyciMetaCyc:MONOMER-8321.
BRENDAi2.7.7.1. 382.
3.6.1.13. 382.

Miscellaneous databases

EvolutionaryTraceiQ55928.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNADM_SYNY3
AccessioniPrimary (citable) accession number: Q55928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.