Q558Z2 (SUN1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sun domain-containing protein 1

Gene names

Name:	sun1
ORF Names:	DDB_G0272869

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have an important role in defining the spacing of the nuclear envelope lumen. Essential for centrosome attachment to the nucleus, maintenance of correct ploidy, proper mitosis, association of the centromere cluster with the centrosome and the maintenance of genome stability. Requires direct chromatin binding for inner nuclear membrane targeting. Ref.2 Ref.3
Subunit structure	Homodimer and homooligomer. Ref.2
Subcellular location	Nucleus membrane; Single-pass membrane protein; Nucleoplasmic side Ref.2 Ref.3.
Disruption phenotype	Shows significant reduced growth. Ref.3
Sequence similarities	Contains 1 SUN domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Membrane Nucleus
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW centromere complex assembly Inferred from mutant phenotype Ref.3. Source: dictyBase centrosome localization Inferred from mutant phenotype Ref.2Ref.3. Source: dictyBase centrosome organization Inferred from mutant phenotype Ref.3. Source: dictyBase mitotic sister chromatid segregation Inferred from mutant phenotype Ref.2Ref.3. Source: dictyBase nucleus organization Inferred from mutant phenotype Ref.2Ref.3. Source: dictyBase
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear inner membrane Inferred from direct assay Ref.2Ref.3. Source: dictyBase nuclear outer membrane Inferred from direct assay Ref.3. Source: dictyBase nucleoplasm Inferred from direct assay Ref.2. Source: dictyBase
Molecular_function	DNA binding Inferred from direct assay Ref.2. Source: dictyBase chromatin binding Inferred from direct assay Ref.2. Source: dictyBase protein homodimerization activity Inferred from direct assay Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	Sun domain-containing protein 1		PRO_0000390618
Regions
	Topological domain	1 – 290	290	Nuclear Potential
	Transmembrane	291 – 311	21	Helical; Potential
	Topological domain	312 – 905	594	Perinuclear space Potential
	Domain	662 – 860	199	SUN
	Coiled coil	170 – 221	52	Potential
	Coiled coil	359 – 456	98	Potential
	Coiled coil	504 – 609	106	Potential
	Coiled coil	864 – 901	38	Potential
	Compositional bias	117 – 120	4	Poly-Asp
	Compositional bias	177 – 213	37	Poly-Gln
	Compositional bias	215 – 221	7	Poly-Asn
	Compositional bias	224 – 227	4	Poly-Asn
	Compositional bias	281 – 284	4	Poly-Asn
	Compositional bias	567 – 572	6	Poly-Ser
	Compositional bias	814 – 819	6	Poly-Thr

Sequences

	Sequence		Length	Mass (Da)
	Q558Z2 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 208FA7368F3280D2	FASTA	905	104,743
	10 20 30 40 50 60 MSGDYKPNYQ SSPSRKRLPL QSKDQASIYK YQTPSTLNLY NNTVNNNSSN NSNNHLLHNS 70 80 90 100 110 120 NPNSSYLYDS SKQYSNQINI RNNSNSNSNT NNITSKKASS SYSINNKVDH NSHNNNDDDD 130 140 150 160 170 180 IEDDVDINYS TNNASSNILH NRFSNSNKDD SYIDYSTDEN PKILKQPQPL YNHLNNQIQQ 190 200 210 220 230 240 QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQRNNNNNS NSSNNNNTST TIKRNNQQID 250 260 270 280 290 300 NNSNKNIISK FIGDPWKNFY YGSNKSLWPF ERNNNSNNSS NNNNKVNFKQ AIWIFIFSVL 310 320 330 340 350 360 FIGCLLGLFS TNFYGIHIYF PSFSTTKTNS PFNSTNNNIQ FSNLITKEQL YPIIDEYFKK 370 380 390 400 410 420 NEILKSYNKL FEKIENDIKY LSEREQYKDI INEIKEELKL VKLSNMDEDR VNQLISKMIN 430 440 450 460 470 480 HYNNNENNKQ ELKELLSKSI EELTKLKSDS KEQLIQISTE SMNQLGQLKS ESINQLGQVK 490 500 510 520 530 540 SESIDKFQST LKSLSKEEQS KIEREFNHQF NQLNKDADQL LSQHSLKIEK LREEINENQQ 550 560 570 580 590 600 SSLLKLTQEY KQLEERLKEF SSKLQQSISS SSMDQFESWK LVFIKDIEER INKESSKLTN 610 620 630 640 650 660 QYIQLTQQFT KIQSFIKDNP SIDSLTNTIE SLEGIKLLIE DILEVYSADK IAKVDYALGL 670 680 690 700 710 720 AGASIEYNAL HYRVSETYPP IKGSGSGSGS GGANGNSLGL YYYNLATNWI FPQPKPNPPE 730 740 750 760 770 780 TILDPMVNTG SCWGFYTGNG TIVIRLAKKI AITEVTMEHI SSNISHHIDS APKEFQVFGL 790 800 810 820 830 840 INSSDIGQSL GVFTYDTTIN RHLQTFKVNK IQSTTTTTTN QDQNDDDNIQ EFSHVALRIL 850 860 870 880 890 900 SNHGYRYTCI YRFRVHGYQI PHPEQEQIQI IQEEQSFKQE EINQQQIEQI EQIEQIEKQQ QSDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Dictyostelium Sun-1 connects the centrosome to chromatin and ensures genome stability." Xiong H., Rivero F., Euteneuer U., Mondal S., Mana-Capelli S., Larochelle D., Vogel A., Gassen B., Noegel A.A. Traffic 9:708-724(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, SUBUNIT.
[3]	"Dictyostelium Sun1 is a dynamic membrane protein of both nuclear membranes and required for centrosomal association with clustered centromeres." Schulz I., Baumann O., Samereier M., Zoglmeier C., Graef R. Eur. J. Cell Biol. 88:621-638(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL71072.1.
RefSeq	XP_644924.1. XM_639832.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0219949; DDB0219949; DDB_G0272869.
GeneID	8618603.
KEGG	ddi:DDB_G0272869.
Organism-specific databases
dictyBase	DDB_G0272869. sun1.
Phylogenomic databases
eggNOG	NOG285464.
InParanoid	Q558Z2.
OMA	VYSADKI.
Family and domain databases
InterPro	IPR012919. Sad1_UNC_C. [Graphical view]
Pfam	PF07738. Sad1_UNC. 1 hit. [Graphical view]
PROSITE	PS51469. SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUN1_DICDI

Accession

Primary (citable) accession number: Q558Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)