Q558Z0 (SYRC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable arginine--tRNA ligase, cytoplasmic
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS1
Synonyms:	argS
ORF Names:	DDB_G0272867

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis By similarity.
Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	phagocytic vesicle Inferred from direct assay PubMed 19482547. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Probable arginine--tRNA ligase, cytoplasmic		PRO_0000327521
Regions
	Motif	121 – 132	12	"HIGH" region

Sequences

	Sequence		Length	Mass (Da)
	Q558Z0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 557A91F67E0F08DE	FASTA	589	67,044
	10 20 30 40 50 60 MFQEEIAKQL SVLTEIEANK ILECIESTKN KDMADFAVPI PKLNKFKKLV GKPDQLAIDF 70 80 90 100 110 120 ASKIQLNDCI QGASATGNYL NFKVNRLLQV QEILKEVINQ KEKYGMTEQG KGKKVIVEFS 130 140 150 160 170 180 SPNIAKPFHA GHLRSTIIGN FMVNLFNELA YETVSMNYLG DWGKQYGLLA VGFEKYGSEE 190 200 210 220 230 240 ELLADPIKHL YNVYVQINGE AEKEEEAKKK YAEEIAAGVE PTAPLQTTPT IHDTARAYFK 250 260 270 280 290 300 RMEDGDAEAL AIWKRFRDLS IVKYKDIYNR LNVKFDIYAG ESLVTEGMTI EFKKLQDKNL 310 320 330 340 350 360 LEDSQGAKVI DLSKPNKLGK VLVQKTDGTT LYITRDIAAA VDRKNNIGFD KMYYVVASQQ 370 380 390 400 410 420 DFHFRQLFDI LGKMDYQWQK DLTHINYGMV KGMSTRKGTV VFLEDILNKT QKKMLKIMKQ 430 440 450 460 470 480 NEQKFAEIED PEKVADIVGL SAVVIQDFNA KRNKDYDFNW DRMLKSDGDT GPYLQYAHAR 490 500 510 520 530 540 LCSLERKSGF EFNPNANLSL LSEPEAFNLA ITIGRYPEII QLTHNQLEPS TLVGYLFELA 550 560 570 580 HAVSSAHQVL WIKDREKDVA EARFVLYWAA KVILGSGLRI LGLVPLERM

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL71071.1.
RefSeq	XP_644926.1. XM_639834.1.
3D structure databases
HSSP	HSSP built from PDB template 1BS2 based on UniProtKB Q05506.
ProteinModelPortal	Q558Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231324.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231324; DDB0231324; DDB_G0272867.
GeneID	8618605.
KEGG	ddi:DDB_G0272867.
Organism-specific databases
dictyBase	DDB_G0272867. argS1.
Phylogenomic databases
eggNOG	COG0018.
KO	K01887.
OMA	CKSMLAW.
ProtClustDB	CLSZ2497265.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYRC_DICDI

Accession

Primary (citable) accession number: Q558Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)