Q558K8 (ICMT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase
EC=2.1.1.100

Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name=PPMT
Prenylcysteine carboxyl methyltransferase
Short name=pcCMT

Gene names

Name:	icmt-1
Synonyms:	icmA
ORF Names:	DDB_G0272799
AND
Name:	icmt-2
Synonyms:	icmA
ORF Names:	DDB_G0273995

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Methylates the C-terminal cysteine residues of small GTPases and the heterotrimeric G protein gamma subunit in response to cAMP. The methylation is required for intercellular signaling and regulation of cAMP waves propagation. It also seems to induce the activity of car1, a G protein-coupled receptor which senses extracellular cAMP during the aggregation phase of development. Ref.1
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Developmental stage	Induced by starvation and expressed throughout development, with a peak at 8 hours after starvation. Ref.1
Disruption phenotype	Cells lacking both icmt-1 and icmt-2 do not propagate cAMP waves in a sustained manner and fail to aggregate, affecting differentiation and development. Ref.1
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of cell polarity Inferred from mutant phenotype Ref.1. Source: dictyBase nuclear envelope organization Inferred from mutant phenotype PubMed 22572958. Source: dictyBase protein localization to nucleus Inferred from mutant phenotype PubMed 22572958. Source: dictyBase regulation of G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype Ref.1. Source: dictyBase regulation of aggregation involved in sorocarp development Inferred from mutant phenotype Ref.1. Source: dictyBase
Cellular_component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: dictyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from direct assay Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Protein-S-isoprenylcysteine O-methyltransferase		PRO_0000328175
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	184 – 204	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q558K8 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 3F6961685B01C5DE	FASTA	237	27,379
	10 20 30 40 50 60 MDQSEIVKLN KIKAKSAWLK KGAARSSAIS CGLGIGIGFG IALFIFSQTL RGFGIYLAGL 70 80 90 100 110 120 CTFHMWEYIW VTMYHPDKLS SKSFLLNHSP QFNMALLISF IEFWIEWYFF PSLKTFSLWW 130 140 150 160 170 180 VGAICMVFGQ IVRSVAMDTA GSNFTHLVQE EKRDDHVLVT NGIYQYMRHP SYFGWFVWSV 190 200 210 220 230 STQVILMNPI SIIGFGWASW SFFSQRIENE EDYLIQFFGK SYKDYKKSVW SGIPGIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isoprenylcysteine carboxy methylation is essential for development in Dictyostelium discoideum." Chen Y., McQuade K.J., Guan X.-J., Thomason P.A., Wert M.S., Stock J.B., Cox E.C. Mol. Biol. Cell 18:4106-4118(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF487784 Genomic DNA. Translation: AAL99548.1. AAFI02000011 Genomic DNA. Translation: EAL70431.1. AAFI02000009 Genomic DNA. Translation: EAL71037.2.
RefSeq	XP_644356.1. XM_639264.1. XP_645058.2. XM_639966.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185114; DDB0185114; DDB_G0272799. DDB0252724; DDB0252724; DDB_G0273995.
GeneID	8618733. 8619243.
KEGG	ddi:DDB_G0272799. ddi:DDB_G0273995.
Organism-specific databases
dictyBase	DDB_G0272799. icmA-1. DDB_G0273995. icmA-2.
Phylogenomic databases
eggNOG	COG2020.
KO	K00587.
OMA	QWILANP.
ProtClustDB	CLSZ2729217.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICMT_DICDI

Accession

Primary (citable) accession number: Q558K8
Secondary accession number(s): Q86AM7, Q8T623

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)