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Q558K8

- ICMT_DICDI

UniProt

Q558K8 - ICMT_DICDI

Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

icmt-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    Methylates the C-terminal cysteine residues of small GTPases and the heterotrimeric G protein gamma subunit in response to cAMP. The methylation is required for intercellular signaling and regulation of cAMP waves propagation. It also seems to induce the activity of car1, a G protein-coupled receptor which senses extracellular cAMP during the aggregation phase of development.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

    Cofactori

    Divalent cations. Probably zinc By similarity.By similarity

    GO - Molecular functioni

    1. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: dictyBase

    GO - Biological processi

    1. C-terminal protein methylation Source: dictyBase
    2. establishment of cell polarity Source: dictyBase
    3. nuclear envelope organization Source: dictyBase
    4. protein localization to nucleus Source: dictyBase
    5. regulation of aggregation involved in sorocarp development Source: dictyBase
    6. regulation of G-protein coupled receptor protein signaling pathway Source: dictyBase

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
    Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase
    Prenylated protein carboxyl methyltransferase
    Short name:
    PPMT
    Prenylcysteine carboxyl methyltransferase
    Short name:
    pcCMT
    Gene namesi
    Name:icmt-1
    Synonyms:icmA
    ORF Names:DDB_G0272799
    AND
    Name:icmt-2
    Synonyms:icmA
    ORF Names:DDB_G0273995
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272799. icmA-1.
    DDB_G0273995. icmA-2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: dictyBase
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking both icmt-1 and icmt-2 do not propagate cAMP waves in a sustained manner and fail to aggregate, affecting differentiation and development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Protein-S-isoprenylcysteine O-methyltransferasePRO_0000328175Add
    BLAST

    Expressioni

    Developmental stagei

    Induced by starvation and expressed throughout development, with a peak at 8 hours after starvation.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0185114.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei53 – 7321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei92 – 11221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei116 – 13621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei184 – 20421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2020.
    KOiK00587.
    OMAiSTAMIHA.
    PhylomeDBiQ558K8.

    Family and domain databases

    InterProiIPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view]
    PfamiPF04140. ICMT. 1 hit.
    [Graphical view]
    PROSITEiPS51564. SAM_ICMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q558K8-1 [UniParc]FASTAAdd to Basket

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    MDQSEIVKLN KIKAKSAWLK KGAARSSAIS CGLGIGIGFG IALFIFSQTL    50
    RGFGIYLAGL CTFHMWEYIW VTMYHPDKLS SKSFLLNHSP QFNMALLISF 100
    IEFWIEWYFF PSLKTFSLWW VGAICMVFGQ IVRSVAMDTA GSNFTHLVQE 150
    EKRDDHVLVT NGIYQYMRHP SYFGWFVWSV STQVILMNPI SIIGFGWASW 200
    SFFSQRIENE EDYLIQFFGK SYKDYKKSVW SGIPGIH 237
    Length:237
    Mass (Da):27,379
    Last modified:May 2, 2006 - v2
    Checksum:i3F6961685B01C5DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487784 Genomic DNA. Translation: AAL99548.1.
    AAFI02000011 Genomic DNA. Translation: EAL70431.1.
    AAFI02000009 Genomic DNA. Translation: EAL71037.2.
    RefSeqiXP_644356.1. XM_639264.1.
    XP_645058.2. XM_639966.2.

    Genome annotation databases

    EnsemblProtistsiDDB0185114; DDB0185114; DDB_G0272799.
    DDB0252724; DDB0252724; DDB_G0273995.
    GeneIDi8618733.
    8619243.
    KEGGiddi:DDB_G0272799.
    ddi:DDB_G0273995.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487784 Genomic DNA. Translation: AAL99548.1 .
    AAFI02000011 Genomic DNA. Translation: EAL70431.1 .
    AAFI02000009 Genomic DNA. Translation: EAL71037.2 .
    RefSeqi XP_644356.1. XM_639264.1.
    XP_645058.2. XM_639966.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185114 ; DDB0185114 ; DDB_G0272799 .
    DDB0252724 ; DDB0252724 ; DDB_G0273995 .
    GeneIDi 8618733.
    8619243.
    KEGGi ddi:DDB_G0272799.
    ddi:DDB_G0273995.

    Organism-specific databases

    dictyBasei DDB_G0272799. icmA-1.
    DDB_G0273995. icmA-2.

    Phylogenomic databases

    eggNOGi COG2020.
    KOi K00587.
    OMAi STAMIHA.
    PhylomeDBi Q558K8.

    Miscellaneous databases

    PROi Q558K8.

    Family and domain databases

    InterProi IPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view ]
    Pfami PF04140. ICMT. 1 hit.
    [Graphical view ]
    PROSITEi PS51564. SAM_ICMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isoprenylcysteine carboxy methylation is essential for development in Dictyostelium discoideum."
      Chen Y., McQuade K.J., Guan X.-J., Thomason P.A., Wert M.S., Stock J.B., Cox E.C.
      Mol. Biol. Cell 18:4106-4118(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiICMT_DICDI
    AccessioniPrimary (citable) accession number: Q558K8
    Secondary accession number(s): Q86AM7, Q8T623
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3