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Q55894

- THIC_SYNY3

UniProt

Q55894 - THIC_SYNY3

Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

    Catalytic activityi

    5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateUniRule annotation
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei139 – 1391SubstrateUniRule annotation
    Binding sitei175 – 1751SubstrateUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Metal bindingi279 – 2791ZincUniRule annotation
    Binding sitei302 – 3021SubstrateUniRule annotation
    Metal bindingi343 – 3431ZincUniRule annotation
    Metal bindingi423 – 4231Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi426 – 4261Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi431 – 4311Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. lyase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
    Alternative name(s):
    Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
    Short name:
    HMP-P synthaseUniRule annotation
    Short name:
    HMP-phosphate synthaseUniRule annotation
    Short name:
    HMPP synthaseUniRule annotation
    Thiamine biosynthesis protein ThiCUniRule annotation
    Gene namesi
    Name:thiCUniRule annotation
    Ordered Locus Names:slr0118
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459Phosphomethylpyrimidine synthasePRO_0000152842Add
    BLAST

    Proteomic databases

    PaxDbiQ55894.

    Interactioni

    Protein-protein interaction databases

    IntActiQ55894. 1 interaction.
    STRINGi1148.slr0118.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55894.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni195 – 1973Substrate bindingUniRule annotation
    Regioni236 – 2394Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ThiC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0422.
    HOGENOMiHOG000224483.
    KOiK03147.
    OMAiWERQFEL.
    OrthoDBiEOG6NWBM5.
    PhylomeDBiQ55894.

    Family and domain databases

    HAMAPiMF_00089. ThiC.
    InterProiIPR002817. ThiC.
    [Graphical view]
    PfamiPF01964. ThiC. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00190. thiC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q55894-1 [UniParc]FASTAAdd to Basket

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    MRTAWVAKRQ GQTNVSQMHY ARKGVITEEM DYVAKRENLP VELIKDEVAR    50
    GRMIIPANIN HTNLEPMAIG IASKCKVNAN IGASPNSSNI DEEVEKLLLS 100
    VKYGADTVMD LSTGGGDLDV IRTAIINASP VPIGTVPIYQ ALESVHGSIE 150
    NLTPDDFLHI IEKHAQQGVD YMTIHAGLLI EYLPLVKSRI TGIVSRGGGI 200
    IAKWMLHHHK QNPLYTHFDE IIEIFKKYDV SFSLGDSLRP GCTHDASDDA 250
    QLSELKTLGQ LTRRAWEHDV QVMVEGPGHV PIDQIEFNVK KQMEECSEAP 300
    FYVLGPLVTD IAPGYDHITS AIGAAIAGWH GTAMLCYVTP KEHLGLPNAE 350
    DVRNGLIAYK IAAHAADIAR HRPGARDRDD ELSKARYNFD WNRQFELSLD 400
    PERAKEYHDE TLPADIYKTA EFCSMCGPKF CPMQTKVDAE MLEELEVFLA 450
    KDKEMVSQR 459
    Length:459
    Mass (Da):51,137
    Last modified:November 1, 1996 - v1
    Checksum:i4819258FC5DBADA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA10656.1.
    PIRiS76712.
    RefSeqiNP_442586.1. NC_000911.1.
    YP_005652647.1. NC_017277.1.
    YP_007452462.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA10656; BAA10656; BAA10656.
    GeneIDi952254.
    KEGGisyn:slr0118.
    syy:SYNGTS_2694.
    syz:MYO_127200.
    PATRICi23842900. VBISynSp132158_2988.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA10656.1 .
    PIRi S76712.
    RefSeqi NP_442586.1. NC_000911.1.
    YP_005652647.1. NC_017277.1.
    YP_007452462.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali Q55894.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q55894. 1 interaction.
    STRINGi 1148.slr0118.

    Proteomic databases

    PaxDbi Q55894.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA10656 ; BAA10656 ; BAA10656 .
    GeneIDi 952254.
    KEGGi syn:slr0118.
    syy:SYNGTS_2694.
    syz:MYO_127200.
    PATRICi 23842900. VBISynSp132158_2988.

    Phylogenomic databases

    eggNOGi COG0422.
    HOGENOMi HOG000224483.
    KOi K03147.
    OMAi WERQFEL.
    OrthoDBi EOG6NWBM5.
    PhylomeDBi Q55894.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .

    Family and domain databases

    HAMAPi MF_00089. ThiC.
    InterProi IPR002817. ThiC.
    [Graphical view ]
    Pfami PF01964. ThiC. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00190. thiC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
      Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
      DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27184 / PCC 6803 / N-1.
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiTHIC_SYNY3
    AccessioniPrimary (citable) accession number: Q55894
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3