Reviewed, UniProtKB/Swiss-Prot Q55880 (RLMN_SYNY3)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase N
    EC=2.1.1.-
Alternative name(s):
    23S rRNA m2A2503 methyltransferase

Gene names

Name:	rlmN
Ordered Locus Names:	sll0098

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates position 2 of adenine 2503 in 23S rRNA By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Ribosomal RNA large subunit methyltransferase N HAMAP MF_01849		PRO_0000171930
Sites
	Metal binding	113	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	117	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	120	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55880-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B2624A3808B7E84B	FASTA	350	38,857
	10 20 30 40 50 60 MAPSPAPLLS LSLPELTDWV QTTGQPAYRG KQIHQWLYQK GARSLTAMTD LPKVWREKNV 70 80 90 100 110 120 HYPIGRSVID HCAVAPDHTR KYLLRLADGL IIETVGIPSS KRLTVCVSSQ VGCAMDCNFC 130 140 150 160 170 180 ATGKGGFIRN LESHEIVDQV LTVQEEFHER VSNVVFMGMG EPLLNLPQVV KAVECLNQVV 190 200 210 220 230 240 GIGQRALTIS TVGLPGKIRQ LADRHLQVTF AVSLHAPNQT LRQSLIPSAR HYPLEQLLAD 250 260 270 280 290 300 CRAYVETTGR RVTFEYVLLA GVNDQPVHAE ELAQKLRGFQ THVNLIPYNP ISEVDYQRPT 310 320 330 340 350 EAQINQFAQV LSDHRIAVSV RYSRGVQADA ACGQLRASRK EELAELTPMA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10642.1.
PIR	S76698.
RefSeq	NP_442572.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55880.
Genome annotation databases
GeneID	952268.
GenomeReviews	Gene locus sll0098 in contig BA000022_GR.
KEGG	syn:sll0098.
NMPDR	fig|1148.1.peg.2673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55880.
OMA	TFCSTAQ
Enzyme and pathway databases
BioCyc	SSP1148:SLL0098-MON.
Family and domain databases
HAMAP	MF_01849. [Tree]
InterPro	IPR007197. Radical_SAM. IPR004383. rRNA_lsu_MTrfase_RlmN. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
TIGRFAMs	TIGR00048. CHP48. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMN_SYNY3

Accession

Primary (citable) accession number: Q55880

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)