Q55860 (COBQ_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobyric acid synthase

Gene names

Name:	cobQ
Synonyms:	cbiP
Ordered Locus Names:	slr0618

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00028
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00028
Sequence similarities	Belongs to the CobB/CobQ family. CobQ subfamily. Contains 1 GATase cobBQ-type domain.
Sequence caution	The sequence BAA10617.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Domain	Glutamine amidotransferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Cobyric acid synthase HAMAP-Rule MF_00028		PRO_0000141336
Regions
	Domain	250 – 439	190	GATase cobBQ-type
Sites
	Active site	331	1	Nucleophile By similarity
	Active site	431	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55860 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: AA265C71E2829299	FASTA	498	54,813
	10 20 30 40 50 60 MKAIMVVGTT SHAGKSFLTT GLCRLLRRRG DRVTPFKGQN MALNAFVTID GGEMGHAQAV 70 80 90 100 110 120 QAWAAGTVPR VAMNPILLKP QGDMTSQVIM LGQAVGTTRA QEYYQNYFDR GWQAIANSLE 130 140 150 160 170 180 TLAQDFDVVV CEGAGSPAEI NLKHRDLTNM RVATHLQAAT ILIADIDRGG VFAHVVGTLQ 190 200 210 220 230 240 LLEPEERKLI KGIVINKFRG QRSLLDSGIT WLEEYTGIPV LGVIPYGQNL FSAEDSLDIL 250 260 270 280 290 300 ERSGRPSKED IRIVVLRLPR IANFTDFEPL EAESGVYVDY LPLEKQLGNP DAVIIPGSKT 310 320 330 340 350 360 TIADLQALQN SGMAQQIMDY AQAGGTVMGI CGGYQMLGQT IADPENIEGN VSNCQGLGLL 370 380 390 400 410 420 PLTTVINQEK ITRQCQTITH APPSGLTVTG YEIHQGLSRR SEHSQDFLPM FDQEELGMVN 430 440 450 460 470 480 AQQNLWGCYL HGLFDNGAWR RCWLNNLRQR RGLDPLPTSI GNYQEQREGM LDSLADFVET 490 HVNLEPILQS LPEENETP

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10617.1. Different initiation.
PIR	S76673.
RefSeq	NP_442547.1. NC_000911.1. YP_007452423.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55860.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55860. 1 interaction.
STRING	1148.slr0618.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10617; BAA10617; BAA10617.
GeneID	14618104. 954919.
KEGG	syn:slr0618.
PATRIC	23842818. VBISynSp132158_2948.
Phylogenomic databases
eggNOG	COG1492.
HOGENOM	HOG000224803.
KO	K02232.
OMA	AHTDLNP.
ProtClustDB	PRK00784.
Enzyme and pathway databases
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00028. CobQ.
InterPro	IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR004459. CobQ_synth. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00313. cobQ. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBQ_SYNY3

Accession

Primary (citable) accession number: Q55860

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program