Q55849 (BIOD_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	slr0523

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187992
Regions
	Nucleotide binding	117 – 120	4	ATP By similarity
	Nucleotide binding	177 – 178	2	ATP By similarity
	Nucleotide binding	209 – 211	3	ATP By similarity
Sites
	Metal binding	25	1	Magnesium 2 By similarity
	Metal binding	56	1	Magnesium 2 By similarity
	Metal binding	117	1	Magnesium 2 By similarity
	Binding site	52	1	Substrate By similarity
	Binding site	56	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55849 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FF290F9A2AFA3375	FASTA	237	25,805
	10 20 30 40 50 60 MSNFSRAVDQ KTLLVAGCDT GVGKTVTTSA LAAYWWKCGK DQSFGLMKLM QTGLGDDELY 70 80 90 100 110 120 QQLFGHLTRW DVVTPLKFAT PLAPPLAADQ EGKTIDLGVV WQTLQTMQQN HDHVLVEALG 130 140 150 160 170 180 SLGSPVTHEL TVADIAALWR LETILVVPVQ LGAMGQAIAQ VALARQTKVK LKGLVLSCAS 190 200 210 220 230 PEAEGKVEDW ATPAMLESFT HLPVLGIVPY LTESERENLS RLAEITARFG LEKLAYF

References

[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10605.1.
PIR	S76661.
RefSeq	NP_442535.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55849.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55849. 2 interactions.
STRING	Q55849.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953292.
GenomeReviews	Gene locus slr0523 in contig BA000022_GR.
KEGG	syn:slr0523.
NMPDR	fig|1148.1.peg.2636.
PATRIC	23842788. VBISynSp132158_2933.
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	ALAAYWQ.
PhylomeDB	Q55849.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0523-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_SYNY3

Accession

Primary (citable) accession number: Q55849

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program