Reviewed, UniProtKB/Swiss-Prot Q55806 (SYT_SYNY3)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	sll0078

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_0000101072
Regions
	Region	197 – 499	303	Catalytic HAMAP MF_00184
Sites
	Metal binding	296	1	Zinc; catalytic By similarity
	Metal binding	347	1	Zinc; catalytic By similarity
	Metal binding	476	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55806-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8AEACAB460A44075	FASTA	603	68,873
	10 20 30 40 50 60 MAKTVVSPDT IALPRTSESE QLKKIRHTTS HVMAMAVQKL FPKAQVTIGP WTETGFYYDF 70 80 90 100 110 120 DVAEPFTEAD LKAIKKEMVK IINKKLPVIR EEISREEAKQ RIESIQEPYK LEILDSIHEP 130 140 150 160 170 180 ITVYHLGDQW WDLCAGPHLE STADINPKAI ALESVAGAYW RGDANKAQLQ RIYGTAWETP 190 200 210 220 230 240 EQLAEYQRRK EEALKRDHRK LGKELGLFIF ADPVGPGLPL WTPKGTIIRT ILEDFLKQEQ 250 260 270 280 290 300 IKRGYLPVVT PHIARVDLFK QSGHWQKYQE DMFPMMAESP EEAAQEMGFV LKPMNCPFHI 310 320 330 340 350 360 QIYKSELRSY RDLPLRLAEF GTVYRYEQSG ELGGLTRVRG FTVDDSHLFV TPDQLDEEFL 370 380 390 400 410 420 SVVDLILTVF KSLQLKNFKA RLSFRDPESD KYIGSDEAWE KAQGAIRRAV QELEMDYFEA 430 440 450 460 470 480 PGEAAFYGPK LDFIFQDALE REWQLGTVQV DYNLPERFDL EYIAADGSRQ RPVMIHRAPF 490 500 510 520 530 540 GSLERLIGIL IEEYAGDFPL WLAPIQVRLL AVSDEFLPFA QSIVQQMQSL GLRAEVDTSG 550 560 570 580 590 600 DRLGKMIRNA EKQKIPVMAV IGAKEVEANA LNIRTRASGE LGEIPVTEVL EKLQATVTNH DTW

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10559.1.
PIR	S76615.
RefSeq	NP_442489.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55806.
Genome annotation databases
GeneID	954903.
GenomeReviews	Gene locus sll0078 in contig BA000022_GR.
KEGG	syn:sll0078.
NMPDR	fig|1148.1.peg.2590.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55806.
OMA	EYTASDG
Enzyme and pathway databases
BioCyc	SSP1148:SLL0078-MON.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_SYNY3

Accession

Primary (citable) accession number: Q55806

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)