Q55801 (RNH_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HI
Short name=RNase HI
EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	slr0080

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00042
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00042
Cofactor	Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00042.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	Ribonuclease HI HAMAP-Rule MF_00042		PRO_0000195408
Regions
	Domain	4 – 147	144	RNase H
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	13	1	Magnesium 2 By similarity
	Metal binding	52	1	Magnesium 1 By similarity
	Metal binding	74	1	Magnesium 1 By similarity
	Metal binding	139	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55801 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F25924F536CBF766	FASTA	160	17,856
	10 20 30 40 50 60 MASTPNSVTL YTDGACSMNP GPGGYGAVIL YGDGRREELS AGYKMTTNNR MEIMGAIAAL 70 80 90 100 110 120 SHLQEPSQVL LYTDSRYMVD AMSKGWAKKW KANGWQRNAK EKAKNPDLWE TMLTLCEKHQ 130 140 150 160 VTFQWVKAHA GNKENERCDR LAVAAYQNNP NLVDEGFGKF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10553.1.
PIR	S76609.
RefSeq	NP_442483.1. NC_000911.1. YP_005652544.1. NC_017277.1. YP_007452359.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55801.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55801. 1 interaction.
STRING	1148.slr0080.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10553; BAA10553; BAA10553.
GeneID	12253856. 14618040. 952423.
KEGG	syn:slr0080. syy:SYNGTS_2591.
PATRIC	23842672. VBISynSp132158_2875.
Phylogenomic databases
eggNOG	COG0328.
HOGENOM	HOG000040465.
KO	K03469.
OMA	MQEIEIF.
Family and domain databases
HAMAP	MF_00042. RNase_H.
InterPro	IPR022892. RNaseH. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. [Graphical view]
Pfam	PF00075. RNase_H. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50879. RNASE_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH_SYNY3

Accession

Primary (citable) accession number: Q55801

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program