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Protein

Ribonuclease HI

Gene

rnhA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Magnesium 1By similarity
Metal bindingi13 – 131Magnesium 2By similarity
Metal bindingi52 – 521Magnesium 1By similarity
Metal bindingi74 – 741Magnesium 1By similarity
Metal bindingi139 – 1391Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HI (EC:3.1.26.4)
Short name:
RNase HI
Gene namesi
Name:rnhA
Ordered Locus Names:slr0080
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Ribonuclease HIPRO_0000195408Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ55801. 1 interaction.
STRINGi1148.SYNGTS_2591.

Structurei

3D structure databases

ProteinModelPortaliQ55801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 147144RNase HAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.Curated
Contains 1 RNase H domain.Curated

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
InParanoidiQ55801.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.
PhylomeDBiQ55801.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTPNSVTL YTDGACSMNP GPGGYGAVIL YGDGRREELS AGYKMTTNNR
60 70 80 90 100
MEIMGAIAAL SHLQEPSQVL LYTDSRYMVD AMSKGWAKKW KANGWQRNAK
110 120 130 140 150
EKAKNPDLWE TMLTLCEKHQ VTFQWVKAHA GNKENERCDR LAVAAYQNNP
160
NLVDEGFGKF
Length:160
Mass (Da):17,856
Last modified:November 1, 1996 - v1
Checksum:iF25924F536CBF766
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10553.1.
PIRiS76609.
RefSeqiYP_005652544.1. NC_017277.1.
YP_007452359.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10553; BAA10553; BAA10553.
KEGGisyn:slr0080.
PATRICi23842672. VBISynSp132158_2875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10553.1.
PIRiS76609.
RefSeqiYP_005652544.1. NC_017277.1.
YP_007452359.1. NC_020286.1.

3D structure databases

ProteinModelPortaliQ55801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55801. 1 interaction.
STRINGi1148.SYNGTS_2591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10553; BAA10553; BAA10553.
KEGGisyn:slr0080.
PATRICi23842672. VBISynSp132158_2875.

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
InParanoidiQ55801.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.
PhylomeDBiQ55801.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiRNH_SYNY3
AccessioniPrimary (citable) accession number: Q55801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.