Q557J6 (DBNL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Drebrin-like protein

Alternative name(s):
Actin-binding protein 1
Short name=Dabp1
Actin-binding protein E

Gene names

Name:	abpE-1
Synonyms:	abp1-1
ORF Names:	DDB_G0273447
AND
Name:	abpE-2
Synonyms:	abp1-2
ORF Names:	DDB_G0273517

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Actin-binding adapter protein. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. Controls pseudopodium number and motility in early stages of chemotactic aggregation. Ref.4
Subcellular location	Cytoplasm › cytoskeleton. Cell projection › pseudopodium Ref.4.
Domain	The SH3 domain mediates the control of pseudopodium number.
Sequence similarities	Belongs to the ABP1 family. Contains 1 ADF-H domain. Contains 1 SH3 domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Domain	Coiled coil SH3 domain
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular component movement Inferred from mutant phenotype Ref.4. Source: dictyBase pseudopodium assembly Inferred from mutant phenotype Ref.4. Source: dictyBase
Cellular component	cell leading edge Inferred from direct assay Ref.4. Source: dictyBase cortical actin cytoskeleton Inferred from direct assay Ref.4. Source: dictyBase extracellular space Inferred from direct assay. Source: dictyBase pseudopodium Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Inferred from sequence or structural similarity Ref.4. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Drebrin-like protein		PRO_0000348228
Regions
	Domain	3 – 131	129	ADF-H
	Domain	422 – 481	60	SH3
	Compositional bias	226 – 236	11	Poly-Gln
	Compositional bias	286 – 289	4	Poly-Gln
	Compositional bias	335 – 338	4	Poly-Gln

Sequences

	Sequence		Length	Mass (Da)
	Q557J6 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 0DA341CED0A949C9	FASTA	481	55,338
	10 20 30 40 50 60 MASLDISDPD ITKYIKLVQD GNPANRWIVF SYVPKSNNKI KFCDSGSGDL KELREELDDS 70 80 90 100 110 120 SIRFAYIRFV INNMPKFVYI PWCGDGVNGP IKGAFSGHAI EFSKSFKPIH HQVNARSEED 130 140 150 160 170 180 IDEKAITAAL NKATGASYDS GSKVQGATKG TFIPQSVSQG REAATKSNAE VKNVINKNDY 190 200 210 220 230 240 NKIQESAEYW KQNQANKSEP AKPTRPEYNL STERDDYWKQ QQAEKQKQQQ QQQQQQASRV 250 260 270 280 290 300 NAPPPSRTVG NKFQEQVSKP TETAPPQPRP APSKGSVLNR FPAATQQQQE PPAPSRPAAP 310 320 330 340 350 360 VPSRVNKPAA PVQPVYQEPV HEEPQYEEPQ YEEEQQQQYE EQPTEEQQYY QEEPQQQYEE 370 380 390 400 410 420 QPTEEQQYYQ EEQQQYEEQP TEEQQYYQEE QQQYEQPTED QQYYQEEQQQ YEQPAEEQYD 430 440 450 460 470 480 QSGYLQAKAL YDYNGENDGD LSFREGDIIT ILDQSDPDGW WQGSLPTGEQ GFFPSNFVQQ L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium Dabp1 tethers PakB to the actin cytoskeleton." de la Roche M.A., Cote G.P. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Abp1 regulates pseudopodium number in chemotaxing Dictyostelium cells." Wang Y., O'Halloran T.J. J. Cell Sci. 119:702-710(2006) [PubMed: 16449327] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY437927 mRNA. Translation: AAR26472.1. AAFI02000010 Genomic DNA. Translation: EAL70678.1. AAFI02000010 Genomic DNA. Translation: EAL70713.1.
RefSeq	XP_644611.1. XM_639519.1. XP_644639.1. XM_639547.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZLM based on UniProtKB Q92882.
ProteinModelPortal	Q557J6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0201562; DDB0201562; DDB_G0273447. DDB0302489; DDB0302489; DDB_G0273517.
GeneID	8618975. 8619002.
GenomeReviews	Gene locus abpE-2 in contig CM000151_GR.
KEGG	ddi:DDB_G0273447. ddi:DDB_G0273517.
Organism-specific databases
dictyBase	DDB_G0273447. abpE-1. DDB_G0273517. abpE-2.
Phylogenomic databases
eggNOG	KOG3655.
GeneTree	EPrGT00050000003419.
OMA	PTEEQQY.
ProtClustDB	CLSZ2431242.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS51263. ADF_H. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DBNL_DICDI

Accession

Primary (citable) accession number: Q557J6
Secondary accession number(s): Q6T3S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 2, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)