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Q557D2 (G6PD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase

Short name=G6PD
EC=1.1.1.49
Gene names
Name:g6pd-1
Synonyms:zwf
ORF Names:DDB_G0273131
AND
Name:g6pd-2
Synonyms:zwf
ORF Names:DDB_G0273639
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentphagocytic vesicle

Inferred from direct assay PubMed 19482547. Source: dictyBase

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Glucose-6-phosphate 1-dehydrogenase HAMAP-Rule MF_00966
PRO_0000312855

Regions

Nucleotide binding17 – 248NADP By similarity
Region181 – 1855Substrate binding By similarity

Sites

Active site2431Proton acceptor By similarity
Binding site511NADP By similarity
Binding site1511NADP; via carbonyl oxygen By similarity
Binding site1511Substrate By similarity
Binding site2191Substrate By similarity
Binding site2381Substrate By similarity
Binding site3371Substrate By similarity
Binding site3421Substrate By similarity
Binding site3731Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q557D2 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 5ECF966E8C5AA31A

FASTA49756,697
        10         20         30         40         50         60 
MTSTPDSRSV LTVIILGASG DLAKKKTYPA LFGLYLRDLL PSNTIIYGYA RSHIEIGDFK 

        70         80         90        100        110        120 
ARISKGLKGD EEKKKQFLNL LHYHSGKYDE KASYDEFEKL ILAEEKKQQG VDKFNRLFYM 

       130        140        150        160        170        180 
AIPPSIFIEV SIGIHGSLIS KNGWSRVIVE KPFGRDLASS RELVSELGKL FKEKDLFRID 

       190        200        210        220        230        240 
HYLGKEMVQN LMVLRFANAV FEPLWSKSHI SSITITFKED IGTEGRGGYF DQFGIIRDVM 

       250        260        270        280        290        300 
QNHLLQVLSL VAMEPPVSLN ADDITNEKVK LLRCIQPIKM SEVVLGQYTS DPEGKIPAYL 

       310        320        330        340        350        360 
DDEGVPKDST TPTYAAAVFH INNPRWRGMP FILKCGKALD ERKTEVRIQF KRPDNFLFSD 

       370        380        390        400        410        420 
DDISRNELVM RIQPGEAVYL KLLSKKPGLE NKIEQTELDL SYRHRFENLD LPDAYERLIL 

       430        440        450        460        470        480 
DSIKGDHNLF VRDDELDVAW QIFTPLLDQI EKEKIKPEPY SFGSRGPKSA DELSKKFGFI 

       490 
RSLGYNWPGN SPQGSKK 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000009 Genomic DNA. Translation: EAL70783.1.
AAFI02000011 Genomic DNA. Translation: EAL70510.1.
RefSeqXP_644436.1. XM_639344.1.
XP_644814.1. XM_639722.1.

3D structure databases

ProteinModelPortalQ557D2.
SMRQ557D2. Positions 11-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231285.

Proteomic databases

PRIDEQ557D2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231285; DDB0231285; DDB_G0273131.
DDB0238739; DDB0238739; DDB_G0273639.
GeneID8618916.
8619061.
KEGGddi:DDB_G0273131.
ddi:DDB_G0273639.

Organism-specific databases

dictyBaseDDB_G0273131. g6pd-1.
DDB_G0273639. g6pd-2.

Phylogenomic databases

eggNOGCOG0364.
OMAIETHPRE.
PhylomeDBQ557D2.

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ557D2.

Entry information

Entry nameG6PD_DICDI
AccessionPrimary (citable) accession number: Q557D2
Secondary accession number(s): Q869V9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 2, 2006
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase