Reviewed, UniProtKB/Swiss-Prot Q55756 (CARB_SYNY3)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase large chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase ammonia chain

Gene names

Name:	carB
Ordered Locus Names:	sll0370

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	1081 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01210
Cofactor	Binds 4 magnesium or manganese ions per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01210 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01210
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carB family. Contains 2 ATP-grasp domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Repeat
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1081	1081	Carbamoyl-phosphate synthase large chain HAMAP MF_01210		PRO_0000145057
Regions
	Domain	133 – 329	197	ATP-grasp 1
	Domain	686 – 878	193	ATP-grasp 2
	Nucleotide binding	160 – 217	58	ATP By similarity
	Nucleotide binding	712 – 769	58	ATP By similarity
	Region	1 – 403	403	Carboxyphosphate synthetic domain HAMAP MF_01210
	Region	404 – 553	150	Oligomerization domain HAMAP MF_01210
	Region	554 – 944	391	Carbamoyl phosphate synthetic domain HAMAP MF_01210
	Region	945 – 1081	137	Allosteric domain HAMAP MF_01210
Sites
	Metal binding	286	1	Magnesium or manganese 1 By similarity
	Metal binding	300	1	Magnesium or manganese 1 By similarity
	Metal binding	300	1	Magnesium or manganese 2 By similarity
	Metal binding	302	1	Magnesium or manganese 2 By similarity
	Metal binding	837	1	Magnesium or manganese 3 By similarity
	Metal binding	849	1	Magnesium or manganese 3 By similarity
	Metal binding	849	1	Magnesium or manganese 4 By similarity
	Metal binding	851	1	Magnesium or manganese 4 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55756-1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 0C2E0D3905B40EE6	FASTA	1,081	119,031
	10 20 30 40 50 60 MPRRNDLNKI MILGAGPIVI GQACEFDYSG TQACKALKEE GYEVVLVNSN PASIMTDPEL 70 80 90 100 110 120 ADRTYIEPLI PEIVEKIIEK ERPDAVLPTM GGQTALNLAV SLSKSGVLEK YGVELIGAKL 130 140 150 160 170 180 PAIEMGEDRE LFKEAMARIG VPVCPSGIAS SIEEARQVAH EIGSYPLIIR PAFTLAGTGG 190 200 210 220 230 240 GIAYNQEEYE EMVQYGLDQS PMSQILVEKS LLGWKEYELE VMRDLADNVV IICSIENFDP 250 260 270 280 290 300 MGVHTGDSIT VAPAQTLTDK EYQRLRDYSI AIIREIGVET GGSNIQFSVN PANGDVIVIE 310 320 330 340 350 360 MNPRVSRSSA LASKATGFPI AKFAAKLAVG YTLNEISNDI TKKTPASFEP TIDYVVTKIP 370 380 390 400 410 420 RFAFEKFPGA EPILNTQMKS VGEAMAIGRT FQESFQKALR SLETGRFGFG CDRHETLPTL 430 440 450 460 470 480 SHLRSQLRTP NPERVFSLHH AFNLGMTVEE IHELTAIDPW FLDKLEDLVK TEKYMKQRSL 490 500 510 520 530 540 KDLTAADLRY IKQQGFGDRQ IAFATKTTED EVRAYRKSLG ITPVYKVVDT CAAEFEAFTP 550 560 570 580 590 600 YYYSTYEPEE CEVLPSDKPK VMILGGGPNR IGQGIEFDYC CCHAAFSLSD AGYETIMVNS 610 620 630 640 650 660 NPETVSTDYD TSDRLYFEPL TKEDVLNIIE AENPVGIIIQ FGGQTPLKLA VPLQKYLNSP 670 680 690 700 710 720 DCPVQTKIWG TSPDSIDTAE DRERFEKILH ELEISQPPNG IARDYEESRV VANRISYPVV 730 740 750 760 770 780 VRPSYVLGGR AMEIVYSDEE LERYMTYAVQ IEPDHPILID KFLENAIEVD VDSLTDSTGK 790 800 810 820 830 840 VVIGSIMEHI EEAGIHSGDS ACSIPYTSLS DNVLTTIRQW TEQLARALNV VGLMNIQYAV 850 860 870 880 890 900 QGDQVYILEA NPRASRTVPY VSKATGRPLA KIASLVMSGK TLEELGVTEE FIPQHVAVKE 910 920 930 940 950 960 AVLPFSKFPG ADTLLGPEMR STGEVMGIDS DFGKAFAKAE LGAGVILATT GTVFVSMSDR 970 980 990 1000 1010 1020 TKEAAVPVVR ELIDLGFKVV ATSGTQKVLR EHGIEGVEVV LKLHEGRPHV IDWIKNGQIQ 1030 1040 1050 1060 1070 1080 FIINTPSGEE SQLDGRTIRR AALDYKLPII TTIAGGKATV AALRSLQDHP LDVKALQDYL G

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10403.1. Different initiation.
PIR	S76557.
RefSeq	NP_442333.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55756. 2 interactions.
STRING	Q55756.
Genome annotation databases
GeneID	951968.
GenomeReviews	Gene locus sll0370 in contig BA000022_GR.
KEGG	syn:sll0370.
NMPDR	fig|1148.1.peg.2434.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55756.
OMA	ATVHSVW
Enzyme and pathway databases
BioCyc	SSP1148:SLL0370-MON.
Family and domain databases
HAMAP	MF_01210. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR005483. CarbamoylP_synth_lsu. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR006275. CarbamoylP_synth_lsu_Gln-dep. IPR005481. CarbamoylP_synth_lsu_N. IPR005480. CarbamoylP_synth_lsu_oligo. IPR011607. MGS. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.40.50.20. Pre-ATP_grasp. 2 hits.
Pfam	PF00289. CPSase_L_chain. 2 hits. PF02786. CPSase_L_D2. 2 hits. PF02787. CPSase_L_D3. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PRINTS	PR00098. CPSASE.
SMART	SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR01369. CPSaseII_lrg. 1 hit.
PROSITE	PS50975. ATP_GRASP. 2 hits. PS00866. CPSASE_1. 1 hit. PS00867. CPSASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARB_SYNY3

Accession

Primary (citable) accession number: Q55756

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	August 2, 2002
Last modified:	November 24, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)