Reviewed, UniProtKB/Swiss-Prot Q55749 (SUMT_SYNY3)

Names and origin

Protein names

Recommended name:
    Uroporphyrinogen-III C-methyltransferase
      Short name=Urogen III methylase
    EC=2.1.1.107
Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
      Short name=UROM

Gene names

Name:	cobA
Ordered Locus Names:	sll0378

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin By similarity.
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Uroporphyrinogen-III C-methyltransferase		PRO_0000150375
Regions
	Region	96 – 98	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	20	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	126	1	S-adenosyl-L-methionine By similarity
	Binding site	180	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55749-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 581847CFF32C7BDE	FASTA	263	27,833
	10 20 30 40 50 60 MVVRLQNGLM GKVYLVGAGP GDPGLITVKG KTLLENAEAV VYDALVSTAI LAMVNPQAEL 70 80 90 100 110 120 IDAGKRRGRH TKLQSETTQL LAQLAEKHAV VVRLKGGDPF IFGRGGEEME DLVKAGIEVE 130 140 150 160 170 180 VVPGITAGIA APAYAQIPLT HRAYSSSVTF VTGHESAGKY RPEVNWAAIA KGSETIVIYM 190 200 210 220 230 240 GVYSLATILP QLMLAGLGED TPIALIRWGT CPEQQKLVGT FATILAQIEV ENFQAPAIVV 250 260 IGAVVNYPAN LRQQLAPILG GVN

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10394.1.
PIR	S76548.
RefSeq	NP_442324.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55749.
Genome annotation databases
GeneID	952490.
GenomeReviews	Gene locus sll0378 in contig BA000022_GR.
KEGG	syn:sll0378.
NMPDR	fig|1148.1.peg.2425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55749.
OMA	KRAGKCS
Enzyme and pathway databases
BioCyc	SSP1148:SLL0378-MON.
Family and domain databases
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit.
Pfam	PF00590. TP_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUMT_SYNY3

Accession

Primary (citable) accession number: Q55749

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)