Reviewed, UniProtKB/Swiss-Prot Q55738 (GYRA_SYNY3)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	slr0417

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 860	860	DNA gyrase subunit A		PRO_0000145269
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55738-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8CE4E91C4CE874C5	FASTA	860	95,073
	10 20 30 40 50 60 MTDSPDRLIA TDLRNEMSQS YLEYAMSVIV GRALPDARDG LKPVHRRILY AMYELGLTPD 70 80 90 100 110 120 RPFRKCARVV GEVLGKYHPH GDTAVYDALV RMAQDFSMRE PLIDGHGNFG SVDNDPPAAM 130 140 150 160 170 180 RYTESRLRPL STNSLLRDIE AETVDFIDNF DGSQQEPTVL PARIPQLLIN GSSGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDGAIALIR NPEITEQELM QIIPGPDFPT GAQILGRSGI REAYLTGRGS 250 260 270 280 290 300 ITMRGVASIE TMEHPGRPDR DAIIVTELPY QTNKAALIER IADLVNDKKI DGIADIRDES 310 320 330 340 350 360 DRDGMRIVIE LKRDAYARVV LNNLYKQTPI QSNFGANLLA LVNGTPEVLT IKKFLTVFWE 370 380 390 400 410 420 FRIETITRRT RYELRKAEER DHLLQGLLIA LDNLDAVIRL IRGAADTASA KTELVEGFSL 430 440 450 460 470 480 SEVQADAILQ MQLRRLTALE ADKITAEHDE LQTKIADFQD ILARRERVNA IIEEELEQIK 490 500 510 520 530 540 AIHATPRRTV IVQEDGELID TDLIANDQAL ILLTEQGYIK RMPASTFGTQ NRATRGKAAA 550 560 570 580 590 600 KIKDDDGVEH FLSCCDHDKV LFFSDRGVVY SLNAYQIPIA SRTARGVPIV QMLPIPKDEK 610 620 630 640 650 660 ITSLVSVSEF DDDTYFIMLT KQGYIKKTAL SAFSNIRANG LIAISLVEGD QLRWVRLAKA 670 680 690 700 710 720 EDSVIIGSQK GMAIHFKADQ DELRALGRAT RGVKSMRLRS GDALISMDIL PSQVVANIAV 730 740 750 760 770 780 GSEDEPDEDL GGDTDAILEE SDNPGPWLLG VTMKGFGKRV PIGQFRLQHR AGLGVKAIRF 790 800 810 820 830 840 KSKDDQLVAL HVVNADDELM IVTNRGIIIR QSVNDISPQS RSATGVRVQR LDADDAIAAV 850 860 ALVPPSGEEE LAEMSESEES

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10380.1.
PIR	S76534.
RefSeq	NP_442310.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55738. 1 interaction.
STRING	Q55738.
Genome annotation databases
GeneID	954623.
GenomeReviews	Gene locus slr0417 in contig BA000022_GR.
KEGG	syn:slr0417.
NMPDR	fig|1148.1.peg.2411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55738.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0417-MON.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_SYNY3

Accession

Primary (citable) accession number: Q55738

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)