Q55729 (SYM_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	slr0649

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Methionine--tRNA ligase HAMAP-Rule MF_01228		PRO_0000139256
Regions
	Motif	16 – 26	11	"HIGH" region HAMAP-Rule MF_01228
	Motif	305 – 309	5	"KMSKS" region HAMAP-Rule MF_01228
Sites
	Metal binding	131	1	Zinc By similarity
	Metal binding	134	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	152	1	Zinc By similarity
	Binding site	308	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55729 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8D97AE90D3F18A34	FASTA	532	61,442
	10 20 30 40 50 60 MLDSSVPTFS VTTPLYYVND VPHLGSAYTT VVADTLARFK RLQGYDVLMI TGTDEHGQKI 70 80 90 100 110 120 QRTAEAQELD PQTHCDRTVV KFKELWRSLN ILYDRFSRTT DPRHLAIVKD FFQRVWDKGD 130 140 150 160 170 180 IYLAQQQGWY CVACEEFKEK RDLLEDNHCP LHPNRKAEWR DEENYFFRLS RYQHPLEELY 190 200 210 220 230 240 AQRPEFIQPS SRRNEVLNFV AQGLQDFSIS RVNLDWGFPL PNDPNHTIYV WFDALLGYVT 250 260 270 280 290 300 ALLDEDEEPN LTNALVKWWP INLHLIGKDI LRFHAVYWPA MLMSAELAIP AQVFGHGFLT 310 320 330 340 350 360 KDGQKMGKSL GNTVDPLDLI NRYGEDAFRY YFLKEIEFGK DGDFNEQRFV NVLNADLAND 370 380 390 400 410 420 LGNLLNRTLG MVKKYCQGQG PQVMATDLAP DNPLKALGSH LGEEVSSAYE RLSFTDACEA 430 440 450 460 470 480 IFTLVRAGNK YIDDMAPWKL FKQGSQKEVE DVLYSVLESI RLSAYLLSPI VPRLSTKIYQ 490 500 510 520 530 QLGFTWDFDQ WRSPLEQAEE FNRHQSWGQL GANQNLPPAQ PIFTKLELPA EE

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10371.1.
PIR	S76525.
RefSeq	NP_442301.1. NC_000911.1. YP_005652361.1. NC_017277.1. YP_007452177.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55729.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55729. 2 interactions.
STRING	1148.slr0649.
Proteomic databases
PaxDb	Q55729.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10371; BAA10371; BAA10371.
GeneID	12253581. 14617855. 952498.
KEGG	syn:slr0649. syy:SYNGTS_2408.
PATRIC	23842260. VBISynSp132158_2670.
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200401.
KO	K01874.
OMA	RINYDLA.
ProtClustDB	PRK11893.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_01228. Met_tRNA_synth_type2.
InterPro	IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_SYNY3

Accession

Primary (citable) accession number: Q55729

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program