Q556Y7 (LYSD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme D
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase D

Gene names

Name:	alyD-1
ORF Names:	DDB_G0273197
AND
Name:	alyD-2
ORF Names:	DDB_G0273731

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has antibacterial activity By similarity. UniProtKB Q554H2
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Cytoplasmic vesicle lumen By similarity UniProtKB Q554H2.
Post-translational modification	Contains disulfide bonds By similarity. UniProtKB Q554H2
Sequence similarities	Belongs to the dictyostelium lysozyme family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Antibiotic Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to Gram-positive bacterium Inferred from sequence or structural similarity PubMed 15640146. Source: UniProtKB peptidoglycan catabolic process Inferred by curator PubMed 15640146. Source: dictyBase
Cellular_component	cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from sequence or structural similarity PubMed 15640146. Source: UniProtKB vesicle lumen Inferred from sequence or structural similarity PubMed 15640146. Source: UniProtKB
Molecular_function	lysozyme activity Inferred from sequence or structural similarity PubMed 15640146. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity UniProtKB Q554H2
	Chain	20 – 260	241	Lysozyme D UniProtKB Q554H2		PRO_0000315920
Regions
	Compositional bias	79 – 149	71	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	Q556Y7 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 8A118309949B6010	FASTA	260	26,057
	10 20 30 40 50 60 MRLLVTLILL IFVLTVSGQY SCSNPCYGNM CCSIPSNNEY YLTTFCDEST ACGTPCSAQT 70 80 90 100 110 120 YFTADSQRFG CGVTLTICST SGGSTTTGGT GSAGTSTSSG SGSGSGSGSG SGSGSGSGTS 130 140 150 160 170 180 GSSSSGSSSG SGSGSSSGSG GSSGSGSGST METGGFYGVC VKAITIDAGP NISVEEEAGM 190 200 210 220 230 240 AIIDASSQIC QDLFGSSSCG WSDKRSITAV QSSVEDGFPV NKPFNVTFED YNKIISNSLI 250 260 LDQQCSNKNN CKYNKLELMK

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000011 Genomic DNA. Translation: EAL70556.1. AAFI02000009 Genomic DNA. Translation: EAL70816.1.
RefSeq	XP_644482.1. XM_639390.1. XP_644768.1. XM_639676.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231280; DDB0231280; DDB_G0273197. DDB0266413; DDB0266413; DDB_G0273731.
GeneID	8618870. 8619106.
KEGG	ddi:DDB_G0273197. ddi:DDB_G0273731.
Organism-specific databases
dictyBase	DDB_G0273197. alyD-1. DDB_G0273731. alyD-2.
Phylogenomic databases
OMA	CDESTAC.
ProtClustDB	CLSZ2430419.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

LYSD_DICDI

Accession

Primary (citable) accession number: Q556Y7
Secondary accession number(s): Q86JT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)