Q556U6 (BIP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Luminal-binding protein 1
Short name=BiP 1

Alternative name(s):
78 kDa glucose-regulated protein homolog
Short name=GRP-78

Gene names

Name:	bip1-1
ORF Names:	DDB_G0273093
AND
Name:	bip1-2
ORF Names:	DDB_G0273813

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 926	900	Luminal-binding protein 1		PRO_0000327977
Regions
	Motif	923 – 926	4	Prevents secretion from ER Potential
	Compositional bias	474 – 479	6	Poly-Gln
	Compositional bias	618 – 622	5	Poly-Gln
	Compositional bias	623 – 629	7	Poly-Glu
	Compositional bias	856 – 860	5	Poly-Lys
	Compositional bias	896 – 900	5	Poly-Gln
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	136	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
	Glycosylation	544	1	N-linked (GlcNAc...) Potential
	Glycosylation	584	1	N-linked (GlcNAc...) Potential
	Glycosylation	596	1	N-linked (GlcNAc...) Potential
	Glycosylation	649	1	N-linked (GlcNAc...) Potential
	Glycosylation	797	1	N-linked (GlcNAc...) Potential
	Glycosylation	881	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q556U6 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: EEA198A7B6B5E111	FASTA	926	104,792
	10 20 30 40 50 60 MVKNIKIFLS LFFVVVLGLL ATTANSMVIG IDLGSQTFKV SLIKPGAFET VLNEQSGRKT 70 80 90 100 110 120 ISSVGWFKDE RLFSSDSFSV WARNPKQNYN LIQAFLGIKY KEGLVEEISN GLPLGFKVKN 130 140 150 160 170 180 DTVRNTVSIV YDDDTNYSAE ELTGMLLRRV KDMASSYAGS SIKDCAITIP PYFTQQQRQA 190 200 210 220 230 240 LLDAAQLAGL NVLSLIHDVN AAALSFAMDR TFLEKNESVI FYDMGARHTS VSLVEFESHN 250 260 270 280 290 300 EQIKGVKKNK TVSSASVKGI EWDEKLGGFD FDMVIVNHLK TLLKKQIPSA NVDDIKITIK 310 320 330 340 350 360 LLKEVGKMKE NLSVNQQAQI FIGSLVDDHD FQATISKQQF EELSQSLIER SLLPLKKLIL 370 380 390 400 410 420 STGIKLKDIE YFEVIGGGVR IPFIQQALKD YLKRDTLDKH LNGDEAMSNG AAFYAASLTH 430 440 450 460 470 480 YFKVKEIKLK DILLNSVDVE INNNIINSGG AGETLLEETE DNEDNELNNS GNEQQQQQQP 490 500 510 520 530 540 TINQGGLKDK KIQLFKVNSK LGIKKTVSFS SENGFSLFLN NPTINNPLAT YTVSNVPTPG 550 560 570 580 590 600 EKYNFTGKPK IHCSFRLTTS GIVVLEKAEA EITVSLIKPQ PQQNKTSSST STTKKNTTTI 610 620 630 640 650 660 ETTDGGSEET TDETTTKQQQ QQEKEEEEEV VVVEKVIEYI QKTIRVPLNF TIKYNGCVEP 670 680 690 700 710 720 LSKELSQESN DRINKLDQVD RILRELRQER NNLESFIYET KDKLESNEEY LKCSTQQERD 730 740 750 760 770 780 QLVEELDKTS AWLSDALDND NTETEEYRKQ LKDIKKKADK IVNRVSQYQL VPVALEELED 790 800 810 820 830 840 TVDKVKPMFE IASKDLNVTA EELKETTDKI QSVSDWVQEK KSEFKLADYS KDLQTSSFDI 850 860 870 880 890 900 KFKLYDLERT IKEILKKKKK PVKPSSSKKD KSSKSSKGKS NSTDEKDQKQ KEQKEQQQQQ 910 920 KEESQFQNDG AEEQQFEDDH KVHDEL

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000011 Genomic DNA. Translation: EAL70597.1. AAFI02000009 Genomic DNA. Translation: EAL70764.1.
RefSeq	XP_644523.1. XM_639431.1. XP_644727.1. XM_639635.1.
3D structure databases
ProteinModelPortal	Q556U6.
ModBase	Search...
Proteomic databases
PRIDE	Q556U6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0266529; DDB0266529; DDB_G0273093. DDB0266530; DDB0266530; DDB_G0273813.
GeneID	8618827. 8619149.
KEGG	ddi:DDB_G0273093. ddi:DDB_G0273813.
Organism-specific databases
dictyBase	DDB_G0273093. DDB_G0273813.
Phylogenomic databases
eggNOG	COG0443.
OMA	VYSQLRD.
ProtClustDB	CLSZ2431182.
Family and domain databases
InterPro	IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIP1_DICDI

Accession

Primary (citable) accession number: Q556U6
Secondary accession number(s): Q86JV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)