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Q556R7

- CF50_DICDI

UniProt

Q556R7 - CF50_DICDI

Protein

Counting factor 50

Gene

cf50-1

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Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (02 May 2006)
      Previous versions | rss
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    Functioni

    Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei125 – 1251By similarity

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB-EC
    2. signal transducer activity Source: dictyBase

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell differentiation Source: dictyBase
    3. cellular component movement Source: dictyBase
    4. cell wall macromolecule catabolic process Source: InterPro
    5. cytolysis Source: UniProtKB-KW
    6. defense response to bacterium Source: UniProtKB-KW
    7. glucose homeostasis Source: dictyBase
    8. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
    9. negative regulation of proteolysis Source: dictyBase
    10. peptidoglycan catabolic process Source: InterPro
    11. signal transduction Source: GOC
    12. single organismal cell-cell adhesion Source: dictyBase

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Counting factor 50 (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramidase 1
    GH family 25 lysozyme 1
    Gene namesi
    Name:cf50-1
    ORF Names:DDB_G0273175
    AND
    Name:cf50-2
    ORF Names:DDB_G0273875
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0273175. cf50-1.
    DDB_G0273875. cf50-2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: dictyBase

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 303279Counting factor 50PRO_0000330647Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.4 Publications

    Protein-protein interaction databases

    STRINGi44689.DDB_0185090.

    Structurei

    3D structure databases

    ProteinModelPortaliQ556R7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni226 – 30378S-G-S motif repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi227 – 30276Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 25 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    OMAiETIAMRI.
    PhylomeDBiQ556R7.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR002053. Glyco_hydro_25.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01183. Glyco_hydro_25. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q556R7-1 [UniParc]FASTAAdd to Basket

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    MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN    50
    QRVIIQVWSG GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS 100
    SAIQSLVSSL KSDGINFNML WIDVEQCDGC WGAESDNADY VQEAVETAQG 150
    LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW YAHYDDNPSF SDTAFYEFGG 200
    WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG SASGSASGSA 250
    SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG 300
    SSI 303
    Length:303
    Mass (Da):30,854
    Last modified:May 2, 2006 - v1
    Checksum:i2B2A3CC1CEE3CE8A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF405695 Genomic DNA. Translation: AAL01036.1.
    AAFI02000011 Genomic DNA. Translation: EAL70628.1.
    AAFI02000009 Genomic DNA. Translation: EAL70805.1.
    RefSeqiXP_644554.1. XM_639462.1.
    XP_644697.1. XM_639605.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185090; DDB0185090; DDB_G0273175.
    DDB0266487; DDB0266487; DDB_G0273875.
    GeneIDi8618796.
    8619180.
    KEGGiddi:DDB_G0273175.
    ddi:DDB_G0273875.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF405695 Genomic DNA. Translation: AAL01036.1 .
    AAFI02000011 Genomic DNA. Translation: EAL70628.1 .
    AAFI02000009 Genomic DNA. Translation: EAL70805.1 .
    RefSeqi XP_644554.1. XM_639462.1.
    XP_644697.1. XM_639605.1.

    3D structure databases

    ProteinModelPortali Q556R7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185090.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185090 ; DDB0185090 ; DDB_G0273175 .
    DDB0266487 ; DDB0266487 ; DDB_G0273875 .
    GeneIDi 8618796.
    8619180.
    KEGGi ddi:DDB_G0273175.
    ddi:DDB_G0273875.

    Organism-specific databases

    dictyBasei DDB_G0273175. cf50-1.
    DDB_G0273875. cf50-2.

    Phylogenomic databases

    eggNOGi NOG12793.
    OMAi ETIAMRI.
    PhylomeDBi Q556R7.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR002053. Glyco_hydro_25.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01183. Glyco_hydro_25. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
      Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
      Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42, IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "A cell-counting factor regulating structure size in Dictyostelium."
      Brock D.A., Gomer R.H.
      Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.
    5. "CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
      Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
      Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.
    6. "A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
      Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
      Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CF COMPLEX.

    Entry informationi

    Entry nameiCF50_DICDI
    AccessioniPrimary (citable) accession number: Q556R7
    Secondary accession number(s): Q86JX6, Q95VT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3