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Q556R7

- CF50_DICDI

UniProt

Q556R7 - CF50_DICDI

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Protein

Counting factor 50

Gene

cf50-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251By similarity

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB-EC
  2. signal transducer activity Source: dictyBase

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell differentiation Source: dictyBase
  3. cellular component movement Source: dictyBase
  4. cell wall macromolecule catabolic process Source: InterPro
  5. cytolysis Source: UniProtKB-KW
  6. defense response to bacterium Source: UniProtKB-KW
  7. glucose homeostasis Source: dictyBase
  8. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
  9. negative regulation of proteolysis Source: dictyBase
  10. peptidoglycan catabolic process Source: InterPro
  11. signal transduction Source: GOC
  12. single organismal cell-cell adhesion Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Counting factor 50 (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase 1
GH family 25 lysozyme 1
Gene namesi
Name:cf50-1
ORF Names:DDB_G0273175
AND
Name:cf50-2
ORF Names:DDB_G0273875
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0273175. cf50-1.
DDB_G0273875. cf50-2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 303279Counting factor 50PRO_0000330647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.4 Publications

Protein-protein interaction databases

STRINGi44689.DDB_0185090.

Structurei

3D structure databases

ProteinModelPortaliQ556R7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni226 – 30378S-G-S motif repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi227 – 30276Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 25 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
InParanoidiQ556R7.
OMAiETIAMRI.
PhylomeDBiQ556R7.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR002053. Glyco_hydro_25.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01183. Glyco_hydro_25. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q556R7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN
60 70 80 90 100
QRVIIQVWSG GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS
110 120 130 140 150
SAIQSLVSSL KSDGINFNML WIDVEQCDGC WGAESDNADY VQEAVETAQG
160 170 180 190 200
LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW YAHYDDNPSF SDTAFYEFGG
210 220 230 240 250
WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG SASGSASGSA
260 270 280 290 300
SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG

SSI
Length:303
Mass (Da):30,854
Last modified:May 2, 2006 - v1
Checksum:i2B2A3CC1CEE3CE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405695 Genomic DNA. Translation: AAL01036.1.
AAFI02000011 Genomic DNA. Translation: EAL70628.1.
AAFI02000009 Genomic DNA. Translation: EAL70805.1.
RefSeqiXP_644554.1. XM_639462.1.
XP_644697.1. XM_639605.1.

Genome annotation databases

EnsemblProtistsiDDB0185090; DDB0185090; DDB_G0273175.
DDB0266487; DDB0266487; DDB_G0273875.
GeneIDi8618796.
8619180.
KEGGiddi:DDB_G0273175.
ddi:DDB_G0273875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405695 Genomic DNA. Translation: AAL01036.1 .
AAFI02000011 Genomic DNA. Translation: EAL70628.1 .
AAFI02000009 Genomic DNA. Translation: EAL70805.1 .
RefSeqi XP_644554.1. XM_639462.1.
XP_644697.1. XM_639605.1.

3D structure databases

ProteinModelPortali Q556R7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185090.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185090 ; DDB0185090 ; DDB_G0273175 .
DDB0266487 ; DDB0266487 ; DDB_G0273875 .
GeneIDi 8618796.
8619180.
KEGGi ddi:DDB_G0273175.
ddi:DDB_G0273875.

Organism-specific databases

dictyBasei DDB_G0273175. cf50-1.
DDB_G0273875. cf50-2.

Phylogenomic databases

eggNOGi NOG12793.
InParanoidi Q556R7.
OMAi ETIAMRI.
PhylomeDBi Q556R7.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR002053. Glyco_hydro_25.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01183. Glyco_hydro_25. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
    Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
    Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42, IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "A cell-counting factor regulating structure size in Dictyostelium."
    Brock D.A., Gomer R.H.
    Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.
  5. "CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
    Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
    Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.
  6. "A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
    Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
    Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CF COMPLEX.

Entry informationi

Entry nameiCF50_DICDI
AccessioniPrimary (citable) accession number: Q556R7
Secondary accession number(s): Q86JX6, Q95VT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 2, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3