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Q556R7 (CF50_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Counting factor 50

EC=3.2.1.17
Alternative name(s):
1,4-beta-N-acetylmuramidase 1
GH family 25 lysozyme 1
Gene names
Name:cf50-1
ORF Names:DDB_G0273175
AND
Name:cf50-2
ORF Names:DDB_G0273875
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Subunit structure

Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA. Ref.1 Ref.4 Ref.5 Ref.6

Subcellular location

Secreted.

Disruption phenotype

In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 25 family.

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionAntimicrobial
Bacteriolytic enzyme
Glycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cell differentiation

Inferred from mutant phenotype Ref.1. Source: dictyBase

cell wall macromolecule catabolic process

Inferred from electronic annotation. Source: InterPro

cellular component movement

Inferred from mutant phenotype Ref.1. Source: dictyBase

cytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

defense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

glucose homeostasis

Inferred from mutant phenotype Ref.5. Source: dictyBase

negative regulation of aggregate size involved in sorocarp development

Inferred from mutant phenotype Ref.1. Source: dictyBase

negative regulation of proteolysis

Inferred from mutant phenotype Ref.1. Source: dictyBase

peptidoglycan catabolic process

Inferred from electronic annotation. Source: InterPro

signal transduction

Inferred from mutant phenotype Ref.1. Source: GOC

single organismal cell-cell adhesion

Inferred from mutant phenotype Ref.1. Source: dictyBase

   Cellular_componentextracellular region

Inferred from direct assay Ref.1. Source: dictyBase

   Molecular_functionlysozyme activity

Inferred from electronic annotation. Source: UniProtKB-EC

signal transducer activity

Inferred from mutant phenotype Ref.1. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.1
Chain25 – 303279Counting factor 50
PRO_0000330647

Regions

Region226 – 30378S-G-S motif repeats
Compositional bias227 – 30276Ser-rich

Sites

Active site1251 By similarity

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q556R7 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 2B2A3CC1CEE3CE8A

FASTA30330,854
        10         20         30         40         50         60 
MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN QRVIIQVWSG 

        70         80         90        100        110        120 
GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS SAIQSLVSSL KSDGINFNML 

       130        140        150        160        170        180 
WIDVEQCDGC WGAESDNADY VQEAVETAQG LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW 

       190        200        210        220        230        240 
YAHYDDNPSF SDTAFYEFGG WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG 

       250        260        270        280        290        300 
SASGSASGSA SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG 


SSI 

« Hide

References

« Hide 'large scale' references
[1]"The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
Development 129:3657-3668(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42, IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"A cell-counting factor regulating structure size in Dictyostelium."
Brock D.A., Gomer R.H.
Genes Dev. 13:1960-1969(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[5]"CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
Eukaryot. Cell 2:788-797(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[6]"A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
Eukaryot. Cell 5:1532-1538(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF405695 Genomic DNA. Translation: AAL01036.1.
AAFI02000011 Genomic DNA. Translation: EAL70628.1.
AAFI02000009 Genomic DNA. Translation: EAL70805.1.
RefSeqXP_644554.1. XM_639462.1.
XP_644697.1. XM_639605.1.

3D structure databases

ProteinModelPortalQ556R7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185090.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185090; DDB0185090; DDB_G0273175.
DDB0266487; DDB0266487; DDB_G0273875.
GeneID8618796.
8619180.
KEGGddi:DDB_G0273175.
ddi:DDB_G0273875.

Organism-specific databases

dictyBaseDDB_G0273175. cf50-1.
DDB_G0273875. cf50-2.

Phylogenomic databases

eggNOGNOG12793.
OMAETIAMRI.
PhylomeDBQ556R7.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR002053. Glyco_hydro_25.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01183. Glyco_hydro_25. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCF50_DICDI
AccessionPrimary (citable) accession number: Q556R7
Secondary accession number(s): Q86JX6, Q95VT3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 2, 2006
Last modified: July 9, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase