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Reviewed, UniProtKB/Swiss-Prot Q556R7 (CF50_DICDI)

Last modified November 3, 2009. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Counting factor 50
    EC=3.2.1.17
Alternative name(s):
    GH family 25 lysozyme 1
    1,4-beta-N-acetylmuramidase 1
Gene names
Name: cf50-1
ORF Names: DDB_G0273175
AND
Name: cf50-2
ORF Names: DDB_G0273875
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Subunit structure

Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA. Ref.1

Subcellular location

Secreted.

Disruption phenotype

In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 25 family.

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.1
Chain25 – 303279Counting factor 50
PRO_0000330647

Regions

Region226 – 30378S-G-S motif repeats
Compositional bias227 – 30276Ser-rich

Sites

Active site1251 By similarity

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q556R7-1 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 2B2A3CC1CEE3CE8A

FASTA30330,854
        10         20         30         40         50         60 
MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN QRVIIQVWSG 

        70         80         90        100        110        120 
GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS SAIQSLVSSL KSDGINFNML 

       130        140        150        160        170        180 
WIDVEQCDGC WGAESDNADY VQEAVETAQG LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW 

       190        200        210        220        230        240 
YAHYDDNPSF SDTAFYEFGG WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG 

       250        260        270        280        290        300 
SASGSASGSA SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG 


SSI 

« Hide

References

« Hide 'large scale' references
[1]"The different components of a multisubunit cell number-counting factor have both unique and overlapping functions."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.
Development 129:3657-3668(2002) [PubMed: 12117815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42, IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed: 12097910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"A cell-counting factor regulating structure size in Dictyostelium."
Brock D.A., Gomer R.H.
Genes Dev. 13:1960-1969(1999) [PubMed: 10444594] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[5]"CF45-1, a secreted protein which participates in Dictyostelium group size regulation."
Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H.
Eukaryot. Cell 2:788-797(2003) [PubMed: 12912898] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
[6]"A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum."
Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.
Eukaryot. Cell 5:1532-1538(2006) [PubMed: 16963635] [Abstract]
Cited for: IDENTIFICATION IN THE CF COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF405695 Genomic DNA. Translation: AAL01036.1.
AC116984 Genomic DNA. Translation: AAO51376.1. Different initiation.
AAFI02000011 Genomic DNA. Translation: EAL70628.1.
AAFI02000009 Genomic DNA. Translation: EAL70805.1.
RefSeqXP_644554.1.
XP_644697.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3395689.
3395852.
GenomeReviewsGene locus cf50-2 in contig CM000151_GR.
KEGGddi:DDBDRAFT_0217351.
ddi:DDB_0185090.

Organism-specific databases

dictyBaseDDB_G0273175. cf50-1.
DDB_G0273875. cf50-2.

Family and domain databases

InterProIPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCF50_DICDI
AccessionPrimary (citable) accession number: Q556R7
Secondary accession number(s): Q86JX6, Q95VT3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 2, 2006
Last modified: November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents