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Protein

Counting factor 50

Gene

cf50-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell-cell adhesion Source: dictyBase
  • cell differentiation Source: dictyBase
  • cell motility Source: dictyBase
  • cell wall macromolecule catabolic process Source: InterPro
  • cytolysis Source: UniProtKB-KW
  • defense response to bacterium Source: UniProtKB-KW
  • glucose homeostasis Source: dictyBase
  • negative regulation of aggregate size involved in sorocarp development Source: dictyBase
  • negative regulation of proteolysis Source: dictyBase
  • peptidoglycan catabolic process Source: InterPro

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Counting factor 50 (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase 1
GH family 25 lysozyme 1
Gene namesi
Name:cf50-1
ORF Names:DDB_G0273175
AND
Name:cf50-2
ORF Names:DDB_G0273875
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0273175 cf50-1
DDB_G0273875 cf50-2

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: dictyBase

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000033064725 – 303Counting factor 50Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi67N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ556R7

Interactioni

Subunit structurei

Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA.4 Publications

Protein-protein interaction databases

STRINGi44689.DDB0266487

Structurei

3D structure databases

ProteinModelPortaliQ556R7
SMRiQ556R7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni226 – 303S-G-S motif repeatsAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi227 – 302Ser-richAdd BLAST76

Sequence similaritiesi

Belongs to the glycosyl hydrolase 25 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IZBF Eukaryota
ENOG41124VU LUCA
InParanoidiQ556R7
OMAiVIDPDYN
PhylomeDBiQ556R7

Family and domain databases

InterProiView protein in InterPro
IPR002053 Glyco_hydro_25
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF01183 Glyco_hydro_25, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q556R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN
60 70 80 90 100
QRVIIQVWSG GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS
110 120 130 140 150
SAIQSLVSSL KSDGINFNML WIDVEQCDGC WGAESDNADY VQEAVETAQG
160 170 180 190 200
LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW YAHYDDNPSF SDTAFYEFGG
210 220 230 240 250
WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG SASGSASGSA
260 270 280 290 300
SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG

SSI
Length:303
Mass (Da):30,854
Last modified:May 2, 2006 - v1
Checksum:i2B2A3CC1CEE3CE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405695 Genomic DNA Translation: AAL01036.1
AAFI02000011 Genomic DNA Translation: EAL70628.1
AAFI02000009 Genomic DNA Translation: EAL70805.1
RefSeqiXP_644554.1, XM_639462.1
XP_644697.1, XM_639605.1

Genome annotation databases

EnsemblProtistsiEAL70628; EAL70628; DDB_G0273875
EAL70805; EAL70805; DDB_G0273175
GeneIDi8618796
8619180
KEGGiddi:DDB_G0273175
ddi:DDB_G0273875

Similar proteinsi

Entry informationi

Entry nameiCF50_DICDI
AccessioniPrimary (citable) accession number: Q556R7
Secondary accession number(s): Q86JX6, Q95VT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 2, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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