Reviewed, UniProtKB/Swiss-Prot Q556R7 (CF50_DICDI)

Names and origin

Protein names

Recommended name:
    Counting factor 50
    EC=3.2.1.17
Alternative name(s):
    GH family 25 lysozyme 1
    1,4-beta-N-acetylmuramidase 1

Gene names

Name:	cf50-1
ORF Names:	DDB_G0273175
AND
Name:	cf50-2
ORF Names:	DDB_G0273875

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell-counting factor that limits the maximum size of the multicellular structure during aggregation. Has a very low lysozyme activity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer. Component of the counting factor (CF) complex, which includes cf60, cf50, cf45-1 and ctnA. Ref.1
Subcellular location	Secreted.
Disruption phenotype	In the absence of CF50, secreted countin is degraded suggesting that it may protect countin from degradation. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.1
	Chain	25 – 303	279	Counting factor 50		PRO_0000330647
Regions
	Region	226 – 303	78	S-G-S motif repeats
	Compositional bias	227 – 302	76	Ser-rich
Sites
	Active site	125	1	By similarity
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	170	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q556R7-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 2B2A3CC1CEE3CE8A	FASTA	303	30,854
	10 20 30 40 50 60 MNKMNNIFLI ISSIILSIVI FVSGECAIDF SSEISVGISD SQWSCLASNN QRVIIQVWSG 70 80 90 100 110 120 GGQYNSNISS VVSAAEQAGF DNIDLYAFLC SECDGNYPAS SAIQSLVSSL KSDGINFNML 130 140 150 160 170 180 WIDVEQCDGC WGAESDNADY VQEAVETAQG LGVLVGVYSS EGEWPQTVGN LSTLSQYPLW 190 200 210 220 230 240 YAHYDDNPSF SDTAFYEFGG WTSPAMKQYI GNTNQCGVSV DLDFYGSGSG CSTSSGSASG 250 260 270 280 290 300 SASGSASGSA SGSNSGSSNS GSSNSGSSNS GSNSGSSNSG SGNSGSSNSG SASGSGTGSG SSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The different components of a multisubunit cell number-counting factor have both unique and overlapping functions." Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H. Development 129:3657-3668(2002) [PubMed: 12117815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-42, IDENTIFICATION IN THE CF COMPLEX, SUBUNIT, DISRUPTION PHENOTYPE.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A cell-counting factor regulating structure size in Dictyostelium." Brock D.A., Gomer R.H. Genes Dev. 13:1960-1969(1999) [PubMed: 10444594] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
[5]	"CF45-1, a secreted protein which participates in Dictyostelium group size regulation." Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R., Gomer R.H. Eukaryot. Cell 2:788-797(2003) [PubMed: 12912898] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
[6]	"A 60-kilodalton protein component of the counting factor complex regulates group size in Dictyostelium discoideum." Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H. Eukaryot. Cell 5:1532-1538(2006) [PubMed: 16963635] [Abstract] Cited for: IDENTIFICATION IN THE CF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF405695 Genomic DNA. Translation: AAL01036.1. AC116984 Genomic DNA. Translation: AAO51376.1. Different initiation. AAFI02000011 Genomic DNA. Translation: EAL70628.1. AAFI02000009 Genomic DNA. Translation: EAL70805.1.
RefSeq	XP_644554.1. XP_644697.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3395689. 3395852.
GenomeReviews	Gene locus cf50-2 in contig CM000151_GR.
KEGG	ddi:DDBDRAFT_0217351. ddi:DDB_0185090.
Organism-specific databases
dictyBase	DDB_G0273175. cf50-1. DDB_G0273875. cf50-2.
Family and domain databases
InterPro	IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CF50_DICDI

Accession

Primary (citable) accession number: Q556R7
Secondary accession number(s): Q86JX6, Q95VT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 2, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)