Q556G3 (GSTA2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glutathione S-transferase alpha-2
EC=2.5.1.18

Alternative name(s):
GST class-alpha 2

Gene names

Name:	gsta2-1
ORF Names:	DDB_G0272632
AND
Name:	gsta2-2
ORF Names:	DDB_G0274081

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.4. Source: dictyBase
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 198	197	Putative glutathione S-transferase alpha-2		PRO_0000350739
Regions
	Domain	5 – 81	77	GST N-terminal
	Domain	83 – 198	116	GST C-terminal
	Region	52 – 53	2	Glutathione binding By similarity
	Region	65 – 66	2	Glutathione binding By similarity
Sites
	Binding site	11	1	Glutathione By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q556G3 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 36104DBFD83CA2D1	FASTA	198	22,538
	10 20 30 40 50 60 MSTSSVPSLT YFQGRGLGQF SRVLLSYLGI PYENITVTEI SDALRATLPY GQLPIYRDGD 70 80 90 100 110 120 FVLTQSSTIA RYIAKKHNFM GKNLEEEFLV DQIVTAIHAD IFPAFNNPVP EKLQKLYEKY 130 140 150 160 170 180 FGSFEKKLQE TGFLVGSSVT LADLYVYVGF DYIRFRGEAA LSSELSDEKY PKIAELKKFF 190 ESNEGVAKYI KERPETKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	Bienvenut W.V., Patel H., Brunton V.G., Frame M.C. Submitted (APR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 23-71; 116-127; 155-172; 178-188 AND 192-198, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000011 Genomic DNA. Translation: EAL70474.1. AAFI02000009 Genomic DNA. Translation: EAL70953.1.
RefSeq	XP_644399.1. XM_639307.1. XP_645014.1. XM_639922.1.
3D structure databases
HSSP	HSSP built from PDB template 2CVD based on UniProtKB O60760.
ProteinModelPortal	Q556G3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231432; DDB0231432; DDB_G0272632. DDB0238181; DDB0238181; DDB_G0274081.
GeneID	8618691. 8619285.
KEGG	ddi:DDB_G0272632. ddi:DDB_G0274081.
Organism-specific databases
dictyBase	DDB_G0272632. DDB_G0274081.
Phylogenomic databases
eggNOG	NOG122057.
OMA	IPYENIT.
ProtClustDB	CLSZ2430358.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA2_DICDI

Accession

Primary (citable) accession number: Q556G3
Secondary accession number(s): Q86AN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)