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Protein

Putative glutathione S-transferase alpha-2

Gene

gsta2-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111GlutathioneBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutathione S-transferase alpha-2 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha 2
Gene namesi
Name:gsta2-1
ORF Names:DDB_G0272632
AND
Name:gsta2-2
ORF Names:DDB_G0274081
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272632.
DDB_G0274081.

Subcellular locationi

GO - Cellular componenti

  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 198197Putative glutathione S-transferase alpha-2PRO_0000350739Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ556G3.

Structurei

3D structure databases

ProteinModelPortaliQ556G3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8177GST N-terminalAdd
BLAST
Domaini83 – 198116GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 532Glutathione bindingBy similarity
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG122057.
InParanoidiQ556G3.
KOiK01830.
OMAiIPYENIT.
PhylomeDBiQ556G3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q556G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSSVPSLT YFQGRGLGQF SRVLLSYLGI PYENITVTEI SDALRATLPY
60 70 80 90 100
GQLPIYRDGD FVLTQSSTIA RYIAKKHNFM GKNLEEEFLV DQIVTAIHAD
110 120 130 140 150
IFPAFNNPVP EKLQKLYEKY FGSFEKKLQE TGFLVGSSVT LADLYVYVGF
160 170 180 190
DYIRFRGEAA LSSELSDEKY PKIAELKKFF ESNEGVAKYI KERPETKF
Length:198
Mass (Da):22,538
Last modified:May 2, 2006 - v1
Checksum:i36104DBFD83CA2D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000011 Genomic DNA. Translation: EAL70474.1.
AAFI02000009 Genomic DNA. Translation: EAL70953.1.
RefSeqiXP_644399.1. XM_639307.1.
XP_645014.1. XM_639922.1.

Genome annotation databases

EnsemblProtistsiDDB0231432; DDB0231432; DDB_G0272632.
DDB0238181; DDB0238181; DDB_G0274081.
GeneIDi8618691.
8619285.
KEGGiddi:DDB_G0272632.
ddi:DDB_G0274081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000011 Genomic DNA. Translation: EAL70474.1.
AAFI02000009 Genomic DNA. Translation: EAL70953.1.
RefSeqiXP_644399.1. XM_639307.1.
XP_645014.1. XM_639922.1.

3D structure databases

ProteinModelPortaliQ556G3.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ556G3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231432; DDB0231432; DDB_G0272632.
DDB0238181; DDB0238181; DDB_G0274081.
GeneIDi8618691.
8619285.
KEGGiddi:DDB_G0272632.
ddi:DDB_G0274081.

Organism-specific databases

dictyBaseiDDB_G0272632.
DDB_G0274081.

Phylogenomic databases

eggNOGiNOG122057.
InParanoidiQ556G3.
KOiK01830.
OMAiIPYENIT.
PhylomeDBiQ556G3.

Miscellaneous databases

PROiQ556G3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. Bienvenut W.V., Patel H., Brunton V.G., Frame M.C.
    Submitted (APR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-15; 23-71; 116-127; 155-172; 178-188 AND 192-198, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiGSTA2_DICDI
AccessioniPrimary (citable) accession number: Q556G3
Secondary accession number(s): Q86AN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 2, 2006
Last modified: April 29, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.