Reviewed, UniProtKB/Swiss-Prot Q556G3 (GSTA2_DICDI)

Names and origin

Protein names

Recommended name:
    Putative glutathione S-transferase alpha-2
    EC=2.5.1.18
Alternative name(s):
    GST class-alpha 2

Gene names

Name:	gsta2-1
ORF Names:	DDB_G0272632
AND
Name:	gsta2-2
ORF Names:	DDB_G0274081

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	phagocytic vesicle Ref.4 Inferred from direct assay. Source: dictyBase
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 198	197	Putative glutathione S-transferase alpha-2		PRO_0000350739
Regions
	Domain	5 – 81	77	GST N-terminal
	Domain	83 – 198	116	GST C-terminal
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q556G3-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 36104DBFD83CA2D1	FASTA	198	22,538
	10 20 30 40 50 60 MSTSSVPSLT YFQGRGLGQF SRVLLSYLGI PYENITVTEI SDALRATLPY GQLPIYRDGD 70 80 90 100 110 120 FVLTQSSTIA RYIAKKHNFM GKNLEEEFLV DQIVTAIHAD IFPAFNNPVP EKLQKLYEKY 130 140 150 160 170 180 FGSFEKKLQE TGFLVGSSVT LADLYVYVGF DYIRFRGEAA LSSELSDEKY PKIAELKKFF 190 ESNEGVAKYI KERPETKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	Bienvenut W.V., Patel H., Brunton V.G., Frame M.C. Submitted (APR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 23-71; 116-127; 155-172; 178-188 AND 192-198, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116984 Genomic DNA. Translation: AAO51292.1. AAFI02000011 Genomic DNA. Translation: EAL70474.1. AAFI02000009 Genomic DNA. Translation: EAL70953.1.
RefSeq	XP_644399.1. XP_645014.1.
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
ModBase	Search...
Genome annotation databases
GeneID	3395480. 3396238.
GenomeReviews	Gene locus gsta2-2 in contig CM000151_GR.
KEGG	ddi:DDB_0231432. ddi:DDB_0238181.
Organism-specific databases
dictyBase	DDB_G0272632. gsta2-1. DDB_G0274081. gsta2-2.
Phylogenomic databases
OMA	THEDAWR.
Family and domain databases
InterPro	IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA2_DICDI

Accession

Primary (citable) accession number: Q556G3
Secondary accession number(s): Q86AN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 2, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)