Q55694 (MNMG_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	sll0202

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00129
Cofactor	FAD By similarity. HAMAP MF_00129
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00129
Subcellular location	Cytoplasm By similarity HAMAP MF_00129.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP MF_00129		PRO_0000117199
Regions
	Nucleotide binding	19 – 24	6	FAD By similarity
	Nucleotide binding	280 – 294	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55694 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 59C62DC20EF31D5B	FASTA	635	70,891
	10 20 30 40 50 60 MTLRSPVEFQ DQFDVIVVGA GHAGCEAALA TARLGCRTLL LTLNLDRIAW QPCNPAVGGP 70 80 90 100 110 120 AKSQLTHEVD ALGGEIGKMA DRTYLQKRVL NISRGPAVWA LRAQTDKREY AAVMKNIVEN 130 140 150 160 170 180 QPNLSIREGM VTDLVLGDND EIQGVQTYFG ACFGAQSVVI TTGTFLGGKI WIGNKSMPAG 190 200 210 220 230 240 RAGEFAAVGL TETLNELGFE TGRLKTGTPA RVDRRSVDYD KLEPQPPDEQ VSWFSFDPEV 250 260 270 280 290 300 WVEREQMNCY LTRTTAKTHQ LIKDNLHLSP IYGGFIDSKG PRYCPSIEDK IVRFADKESH 310 320 330 340 350 360 QIFIEPEGRD IPELYIQGFS TGLPENVQLA MLQTLPGLEN CVMLRPAYAV EYDFLPATQC 370 380 390 400 410 420 YPSLMTKKVA GLFCAGQING TTGYEEAAAQ GLVAGINAAR HCQGKSLIIF SREGSYLGTL 430 440 450 460 470 480 IDDLCTKDLR EPYRMLTSRS EYRLILRSDN ADQRLTPLGR EIGLIDDRRW DLFQTKQANI 490 500 510 520 530 540 TAEKERLYST RIKEQDAVGK EIVDYTQQKI KGSIVLAELL RRPGFHYPDL EKFQLGNEEL 550 560 570 580 590 600 KPEEKTSVEI EIKYSGYIKR QQTQIEQVSR HSQKRLPPGL NYMAIETLSM EAREKLTQFQ 610 620 630 PLTIGQAGRI GGVNPADINA LLVYLETQLR RSVSP

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10223.1.
PIR	S76371.
RefSeq	NP_442153.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55694.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55694.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952636.
GenomeReviews	Gene locus sll0202 in contig BA000022_GR.
KEGG	syn:sll0202.
NMPDR	fig|1148.1.peg.2254.
PATRIC	23841922. VBISynSp132158_2503.
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HBG284774.
OMA	GFDRSPM.
PhylomeDB	Q55694.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0202-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA. [Tree]
InterPro	IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K03495.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. GidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_SYNY3

Accession

Primary (citable) accession number: Q55694

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program