Reviewed, UniProtKB/Swiss-Prot Q55692 (TRMFO_SYNY3)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	sll0204

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000117283
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55692-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 21A6D0CDFF2D22BF	FASTA	456	50,102
	10 20 30 40 50 60 MTTLTPIHVI GGGLAGTEAA WQIAQAGVPV ILTEMRPERL SPAHHSEDLA ELVCSNSFGA 70 80 90 100 110 120 KAGDRAAGLL QTELRQLSSL IITTADRHAV PAGGALAVDR GIFSRSLTEQ VASHPLVELR 130 140 150 160 170 180 RGEVTEIPRE GITVLTTGPL TSPALTEDLQ QFTGMEYLSF FDAASPIVVG DSINKEVAFF 190 200 210 220 230 240 ASRYDKGEAA YLNCPFNREQ YFNFWQALCE AEQAPLKDFD RETAKFFEGC LPIEELAQRG 250 260 270 280 290 300 EDTMRYGPLK PVGLFDARLG DFRDPANKEK KPYAVVQLRQ EDKAGQLWNM VGFQTNLRWG 310 320 330 340 350 360 EQGRVFRLIP GLENAEFVRM GVMHRNTFIN SPQLLTASLH FGDRQTLFAA GQLVGTEGYA 370 380 390 400 410 420 AATAGGWLAG TNAARLALGL PLLTLPATTM MGALFNFISS ASPKHFQPMP PNFGILPELP 430 440 450 QRIRNKQERY GQYRDRALAD LTTWQTSIKS LATCRV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10221.1.
PIR	S76369.
RefSeq	NP_442151.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55692. 1 interaction.
STRING	Q55692.
Genome annotation databases
GeneID	952256.
GenomeReviews	Gene locus sll0204 in contig BA000022_GR.
KEGG	syn:sll0204.
NMPDR	fig|1148.1.peg.2252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55692.
OMA	MKPVGLT
Enzyme and pathway databases
BioCyc	SSP1148:SLL0204-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR002218. GIDA-rel. IPR004417. MTHF-tRNA_MeTrfase_TrmFO. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_SYNY3

Accession

Primary (citable) accession number: Q55692

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)