Q55692 (TRMFO_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74

Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	sll0204

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity. HAMAP MF_01037
Subcellular location	Cytoplasm By similarity HAMAP MF_01037.
Sequence similarities	Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037		PRO_0000117283
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55692 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 21A6D0CDFF2D22BF	FASTA	456	50,102
	10 20 30 40 50 60 MTTLTPIHVI GGGLAGTEAA WQIAQAGVPV ILTEMRPERL SPAHHSEDLA ELVCSNSFGA 70 80 90 100 110 120 KAGDRAAGLL QTELRQLSSL IITTADRHAV PAGGALAVDR GIFSRSLTEQ VASHPLVELR 130 140 150 160 170 180 RGEVTEIPRE GITVLTTGPL TSPALTEDLQ QFTGMEYLSF FDAASPIVVG DSINKEVAFF 190 200 210 220 230 240 ASRYDKGEAA YLNCPFNREQ YFNFWQALCE AEQAPLKDFD RETAKFFEGC LPIEELAQRG 250 260 270 280 290 300 EDTMRYGPLK PVGLFDARLG DFRDPANKEK KPYAVVQLRQ EDKAGQLWNM VGFQTNLRWG 310 320 330 340 350 360 EQGRVFRLIP GLENAEFVRM GVMHRNTFIN SPQLLTASLH FGDRQTLFAA GQLVGTEGYA 370 380 390 400 410 420 AATAGGWLAG TNAARLALGL PLLTLPATTM MGALFNFISS ASPKHFQPMP PNFGILPELP 430 440 450 QRIRNKQERY GQYRDRALAD LTTWQTSIKS LATCRV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10221.1.
PIR	S76369.
RefSeq	NP_442151.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55692.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55692. 1 interaction.
STRING	Q55692.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952256.
GenomeReviews	Gene locus sll0204 in contig BA000022_GR.
KEGG	syn:sll0204.
NMPDR	fig|1148.1.peg.2252.
PATRIC	23841918. VBISynSp132158_2501.
Phylogenomic databases
eggNOG	COG1206.
HOGENOM	HBG285041.
OMA	RFAGQIT.
PhylomeDB	Q55692.
ProtClustDB	PRK05335.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0204-MONOMER.
Family and domain databases
HAMAP	MF_01037. TrmFO. [Tree]
InterPro	IPR004417. Folate-dep_Ribothymidyl_synth. IPR002218. GIDA-rel. [Graphical view]
KO	K04094.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. Gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_SYNY3

Accession

Primary (citable) accession number: Q55692

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program