Reviewed, UniProtKB/Swiss-Prot Q55674 (FUMC_SYNY3)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	slr0018

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161325
Regions
	Region	130 – 133	4	B site By similarity
	Region	140 – 142	3	Substrate binding By similarity
Sites
	Binding site	101	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55674-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7C46A68EA26233C7	FASTA	466	49,687
	10 20 30 40 50 60 MVNSHRLETD SMGSLEVQAD RLWGAQTQRS LMFFDIGSDV MPPDLIRAFA ILKKAAAITN 70 80 90 100 110 120 QDLGKLPADK AELIITAADE IIAGQWLDHF PLRIWQTGSG TQTNMNVNEV IANRAIAICG 130 140 150 160 170 180 GELGSKNPIH PNDHVNMSQS SNDTFPTAMH IAAVAGLQTK LIPSLQALRD SLNEKAECFA 190 200 210 220 230 240 GITKIGRTHL MDAVPLTLGQ EFSGYVAQLD QGLTQINYCL PGLLELALGG TAVGTGLNSH 250 260 270 280 290 300 PQFAKKVAEE IAQLTGYTFI SAPNKFAALA GHEAIAFASG VLKSIAASLM KIANDLRWMG 310 320 330 340 350 360 SGPRCGLGEL ALPANEPGSS IMPGKVNPTQ CEAMTMVCVQ VMGNDATIGF AASQGNFELN 370 380 390 400 410 420 VFKPVIIHNF LHSLHLLSDA CASFRQHLVV GLQVNESKVK DFLDTSLMLV TALNPHIGYD 430 440 450 460 NAALVAKTAF AQGITLKQAA VDLGLLTPAQ FDAWVVPEQM IAPIAD

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10200.1.
PIR	S76348.
RefSeq	NP_442130.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55674. 1 interaction.
STRING	Q55674.
Genome annotation databases
GeneID	952663.
GenomeReviews	Gene locus slr0018 in contig BA000022_GR.
KEGG	syn:slr0018.
NMPDR	fig|1148.1.peg.2231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55674.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0018-MON.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_SYNY3

Accession

Primary (citable) accession number: Q55674

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)