Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fumarate hydratase class II

Gene

fumC

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188SubstrateUniRule annotation1
Active sitei189Proton donor/acceptorUniRule annotation1
Active sitei319UniRule annotation1
Binding sitei320SubstrateUniRule annotation1
Sitei332Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:slr0018
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613251 – 466Fumarate hydratase class IIAdd BLAST466

Proteomic databases

PaxDbiQ55674
PRIDEiQ55674

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiQ55674 1 interactor.
STRINGi1148.SYNGTS_2235

Structurei

3D structure databases

ProteinModelPortaliQ55674
SMRiQ55674
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 101Substrate bindingUniRule annotation3
Regioni130 – 133Substrate binding (B site)UniRule annotation4
Regioni140 – 142Substrate bindingUniRule annotation3
Regioni325 – 327Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000061736
InParanoidiQ55674
KOiK01679
OMAiVSFTDNC
PhylomeDBiQ55674

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.101 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q55674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNSHRLETD SMGSLEVQAD RLWGAQTQRS LMFFDIGSDV MPPDLIRAFA
60 70 80 90 100
ILKKAAAITN QDLGKLPADK AELIITAADE IIAGQWLDHF PLRIWQTGSG
110 120 130 140 150
TQTNMNVNEV IANRAIAICG GELGSKNPIH PNDHVNMSQS SNDTFPTAMH
160 170 180 190 200
IAAVAGLQTK LIPSLQALRD SLNEKAECFA GITKIGRTHL MDAVPLTLGQ
210 220 230 240 250
EFSGYVAQLD QGLTQINYCL PGLLELALGG TAVGTGLNSH PQFAKKVAEE
260 270 280 290 300
IAQLTGYTFI SAPNKFAALA GHEAIAFASG VLKSIAASLM KIANDLRWMG
310 320 330 340 350
SGPRCGLGEL ALPANEPGSS IMPGKVNPTQ CEAMTMVCVQ VMGNDATIGF
360 370 380 390 400
AASQGNFELN VFKPVIIHNF LHSLHLLSDA CASFRQHLVV GLQVNESKVK
410 420 430 440 450
DFLDTSLMLV TALNPHIGYD NAALVAKTAF AQGITLKQAA VDLGLLTPAQ
460
FDAWVVPEQM IAPIAD
Length:466
Mass (Da):49,687
Last modified:November 1, 1996 - v1
Checksum:i7C46A68EA26233C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA10200.1
PIRiS76348

Genome annotation databases

EnsemblBacteriaiBAA10200; BAA10200; BAA10200
KEGGisyn:slr0018

Similar proteinsi

Entry informationi

Entry nameiFUMC_SYNY3
AccessioniPrimary (citable) accession number: Q55674
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: November 1, 1996
Last modified: January 31, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome