Reviewed, UniProtKB/Swiss-Prot Q55663 (DXR_SYNY3)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	sll0019

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163721
Regions
	Nucleotide binding	8 – 37	30	NADP By similarity
Sites
	Metal binding	152	1	Divalent metal cation By similarity
	Metal binding	154	1	Divalent metal cation By similarity
	Metal binding	223	1	Divalent metal cation By similarity
	Binding site	127	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55663-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D5F94C98EC6AE590	FASTA	394	42,532
	10 20 30 40 50 60 MVKRISILGS TGSIGTQTLD IVTHHPDAFQ VVGLAAGGNV ALLAQQVAEF RPEIVAIRQA 70 80 90 100 110 120 EKLEDLKAAV AELTDYQPMY VVGEEGVVEV ARYGDAESVV TGIVGCAGLL PTMAAIAAGK 130 140 150 160 170 180 DIALANKETL IAGAPVVLPL VEKMGVKLLP ADSEHSAIFQ CLQGVPEGGL RRIILTASGG 190 200 210 220 230 240 AFRDLPVERL PFVTVQDALK HPNWSMGQKI TIDSATLMNK GLEVIEAHYL FGLDYDHIDI 250 260 270 280 290 300 VIHPQSIIHS LIEVQDTSVL AQLGWPDMRL PLLYALSWPE RIYTDWEPLD LVKAGSLSFR 310 320 330 340 350 360 EPDHDKYPCM QLAYGAGRAG GAMPAVLNAA NEQAVALFLQ EKISFLDIPR LIEKTCDLYV 370 380 390 GQNTASPDLE TILAADQWAR RTVLENSACV ATRP

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10183.1.
PIR	S76331.
RefSeq	NP_442113.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55663. 1 interaction.
STRING	Q55663.
Genome annotation databases
GeneID	952669.
GenomeReviews	Gene locus sll0019 in contig BA000022_GR.
KEGG	syn:sll0019.
NMPDR	fig|1148.1.peg.2214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0019-MON.
Family and domain databases
HAMAP	MF_00183. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_SYNY3

Accession

Primary (citable) accession number: Q55663

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)