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Q55643 (ISPH_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase

EC=1.17.1.2
Gene names
Name:ispH
Synonyms:lytB
Ordered Locus Names:slr0348
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). HAMAP-Rule MF_00191

Catalytic activity

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191

Cofactor

Binds 1 3Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_00191

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. Ref.4

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. Ref.4

Sequence similarities

Belongs to the IspH family.

Sequence caution

The sequence BAA10159.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3793794-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP-Rule MF_00191
PRO_0000128880

Regions

Region290 – 2923Substrate binding By similarity

Sites

Metal binding391Iron-sulfur (3Fe-4S) By similarity
Metal binding1301Iron-sulfur (3Fe-4S) By similarity
Metal binding2611Iron-sulfur (3Fe-4S) By similarity
Binding site691Substrate By similarity
Binding site1081Substrate By similarity
Binding site1581Substrate By similarity
Binding site2231Substrate By similarity
Binding site3521Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55643 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 4CD0167AF5D4957E

FASTA37942,415
        10         20         30         40         50         60 
MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY ETRQHFPGDR 

        70         80         90        100        110        120 
LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG DVVILPAFGA SVEEMQLLND 

       130        140        150        160        170        180 
RECTIVDTTC PWVSKVWNSV EKHKKKEHTS IIHGKYNHEE TIATSSFAGT YLIVLNMAEA 

       190        200        210        220        230        240 
QKVCDYILHG GDRQEFLDYF ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT 

       250        260        270        280        290        300 
LLQKYGPIEL NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA 

       310        320        330        340        350        360 
VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG ASTPDKVVEE 

       370 
VMKKILAIKE AQPVLEIAG 

« Hide

References

« Hide 'large scale' references
[1]"Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
Cassier-Chauvat C., Poncelet M., Chauvat F.
Gene 195:257-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[3]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
[4]"Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis."
Cunningham F.X. Jr., Lafond T.P., Gantt E.
J. Bacteriol. 182:5841-5848(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PATHWAY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRS76307.
RefSeqNP_442089.1. NC_000911.1.
YP_005652147.1. NC_017277.1.
YP_007451966.1. NC_020286.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ55643. 4 interactions.
STRING1148.slr0348.

Proteomic databases

PaxDbQ55643.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10159; BAA10159; BAA10159.
GeneID952940.
KEGGsyn:slr0348.
syy:SYNGTS_2194.
syz:MYO_122140.
PATRIC23841772. VBISynSp132158_2433.

Phylogenomic databases

eggNOGCOG0761.
KOK03527.
OMADPDNLCF.
OrthoDBEOG6HF624.
PhylomeDBQ55643.

Enzyme and pathway databases

UniPathwayUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPMF_00191. IspH.
InterProIPR003451. LytB.
[Graphical view]
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. ispH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPH_SYNY3
AccessionPrimary (citable) accession number: Q55643
Secondary accession number(s): Q55066
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways