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Reviewed, UniProtKB/Swiss-Prot Q55643 (ISPH_SYNY3)

Last modified February 9, 2010. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2
Gene names
Name: ispH
Synonyms: lytB
Ordered Locus Names: slr0348
OrganismSynechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]
Taxonomic identifier1148 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaChroococcalesSynechocystis

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). HAMAP MF_00191

Catalytic activity

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Cofactor

Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. Ref.4

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. Ref.4

Sequence similarities

Belongs to the ispH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3793794-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191
PRO_0000128880

Sequences

Sequence LengthMass (Da)Tools
Q55643-1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 4CD0167AF5D4957E

FASTA37942,415
        10         20         30         40         50         60 
MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY ETRQHFPGDR 

        70         80         90        100        110        120 
LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG DVVILPAFGA SVEEMQLLND 

       130        140        150        160        170        180 
RECTIVDTTC PWVSKVWNSV EKHKKKEHTS IIHGKYNHEE TIATSSFAGT YLIVLNMAEA 

       190        200        210        220        230        240 
QKVCDYILHG GDRQEFLDYF ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT 

       250        260        270        280        290        300 
LLQKYGPIEL NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA 

       310        320        330        340        350        360 
VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG ASTPDKVVEE 

       370 
VMKKILAIKE AQPVLEIAG 

« Hide

References

« Hide 'large scale' references
[1]"Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
Cassier-Chauvat C., Poncelet M., Chauvat F.
Gene 195:257-266(1997) [PubMed: 9305771] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis."
Cunningham F.X. Jr., Lafond T.P., Gantt E.
J. Bacteriol. 182:5841-5848(2000) [PubMed: 11004185] [Abstract]
Cited for: PATHWAY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRS76307.
RefSeqNP_442089.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ55643. 4 interactions.
STRINGQ55643.

Genome annotation databases

GeneID952940.
GenomeReviewsGene locus slr0348 in contig BA000022_GR.
KEGGsyn:slr0348.
NMPDRfig|1148.1.peg.2190.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0761.
HOGENOMHBG286221.
OMAREENIEF.

Enzyme and pathway databases

BioCycSSP1148:SLR0348-MONOMER.

Family and domain databases

HAMAPMF_00191. IspH.
[Tree]
InterProIPR003451. LytB.
[Graphical view]
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. ispH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPH_SYNY3
AccessionPrimary (citable) accession number: Q55643
Secondary accession number(s): Q55066
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 9, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents