Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation1 Publication
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei69SubstrateUniRule annotation1
Metal bindingi130Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Active sitei160Proton donorUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Metal bindingi261Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei352SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Synonyms:lytB
Ordered Locus Names:slr0348
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001288801 – 3794-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST379

Interactioni

Protein-protein interaction databases

IntActiQ55643. 4 interactors.
STRINGi1148.SYNGTS_2194.

Structurei

3D structure databases

SMRiQ55643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 292Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

InParanoidiQ55643.
KOiK03527.
OMAiTKVHKEA.
PhylomeDBiQ55643.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiView protein in InterPro
IPR003451. LytB/IspH.
PfamiView protein in Pfam
PF02401. LYTB. 1 hit.
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55643-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY
60 70 80 90 100
ETRQHFPGDR LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG
110 120 130 140 150
DVVILPAFGA SVEEMQLLND RECTIVDTTC PWVSKVWNSV EKHKKKEHTS
160 170 180 190 200
IIHGKYNHEE TIATSSFAGT YLIVLNMAEA QKVCDYILHG GDRQEFLDYF
210 220 230 240 250
ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT LLQKYGPIEL
260 270 280 290 300
NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA
310 320 330 340 350
VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG
360 370
ASTPDKVVEE VMKKILAIKE AQPVLEIAG
Length:379
Mass (Da):42,415
Last modified:December 1, 2000 - v2
Checksum:i4CD0167AF5D4957E
GO

Sequence cautioni

The sequence BAA10159 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRiS76307.

Genome annotation databases

EnsemblBacteriaiBAA10159; BAA10159; BAA10159.
KEGGisyn:slr0348.

Similar proteinsi

Entry informationi

Entry nameiISPH_SYNY3
AccessioniPrimary (citable) accession number: Q55643
Secondary accession number(s): Q55066
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 27, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names