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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).

Catalytic activityi

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.

Cofactori

[3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Iron-sulfur (3Fe-4S)By similarity
Binding sitei69 – 691SubstrateBy similarity
Binding sitei108 – 1081SubstrateBy similarity
Metal bindingi130 – 1301Iron-sulfur (3Fe-4S)By similarity
Binding sitei158 – 1581SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Metal bindingi261 – 2611Iron-sulfur (3Fe-4S)By similarity
Binding sitei352 – 3521SubstrateBy similarity

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dimethylallyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-HAMAP
  3. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC:1.17.1.2)
Gene namesi
Name:ispH
Synonyms:lytB
Ordered Locus Names:slr0348
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3793794-hydroxy-3-methylbut-2-enyl diphosphate reductasePRO_0000128880Add
BLAST

Proteomic databases

PaxDbiQ55643.

Interactioni

Protein-protein interaction databases

IntActiQ55643. 4 interactions.
STRINGi1148.slr0348.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 2923Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the IspH family.Curated

Phylogenomic databases

eggNOGiCOG0761.
InParanoidiQ55643.
KOiK03527.
OMAiIIGPRKD.
OrthoDBiEOG6HF624.
PhylomeDBiQ55643.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55643-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY
60 70 80 90 100
ETRQHFPGDR LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG
110 120 130 140 150
DVVILPAFGA SVEEMQLLND RECTIVDTTC PWVSKVWNSV EKHKKKEHTS
160 170 180 190 200
IIHGKYNHEE TIATSSFAGT YLIVLNMAEA QKVCDYILHG GDRQEFLDYF
210 220 230 240 250
ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT LLQKYGPIEL
260 270 280 290 300
NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA
310 320 330 340 350
VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG
360 370
ASTPDKVVEE VMKKILAIKE AQPVLEIAG
Length:379
Mass (Da):42,415
Last modified:November 30, 2000 - v2
Checksum:i4CD0167AF5D4957E
GO

Sequence cautioni

The sequence BAA10159.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRiS76307.
RefSeqiNP_442089.1. NC_000911.1.
YP_005652147.1. NC_017277.1.
YP_007451966.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10159; BAA10159; BAA10159.
KEGGisyn:slr0348.
PATRICi23841772. VBISynSp132158_2433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRiS76307.
RefSeqiNP_442089.1. NC_000911.1.
YP_005652147.1. NC_017277.1.
YP_007451966.1. NC_020286.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55643. 4 interactions.
STRINGi1148.slr0348.

Proteomic databases

PaxDbiQ55643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10159; BAA10159; BAA10159.
KEGGisyn:slr0348.
PATRICi23841772. VBISynSp132158_2433.

Phylogenomic databases

eggNOGiCOG0761.
InParanoidiQ55643.
KOiK03527.
OMAiIIGPRKD.
OrthoDBiEOG6HF624.
PhylomeDBiQ55643.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
    Cassier-Chauvat C., Poncelet M., Chauvat F.
    Gene 195:257-266(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  4. "Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis."
    Cunningham F.X. Jr., Lafond T.P., Gantt E.
    J. Bacteriol. 182:5841-5848(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PATHWAY.

Entry informationi

Entry nameiISPH_SYNY3
AccessioniPrimary (citable) accession number: Q55643
Secondary accession number(s): Q55066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: November 30, 2000
Last modified: March 31, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.