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Q55643

- ISPH_SYNY3

UniProt

Q55643 - ISPH_SYNY3

Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).

    Catalytic activityi

    Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
    Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.

    Cofactori

    Binds 1 3Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi39 – 391Iron-sulfur (3Fe-4S)By similarity
    Binding sitei69 – 691SubstrateBy similarity
    Binding sitei108 – 1081SubstrateBy similarity
    Metal bindingi130 – 1301Iron-sulfur (3Fe-4S)By similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Metal bindingi261 – 2611Iron-sulfur (3Fe-4S)By similarity
    Binding sitei352 – 3521SubstrateBy similarity

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dimethylallyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-HAMAP
    3. terpenoid biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Isoprene biosynthesis

    Keywords - Ligandi

    3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00056; UER00097.
    UPA00059; UER00105.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC:1.17.1.2)
    Gene namesi
    Name:ispH
    Synonyms:lytB
    Ordered Locus Names:slr0348
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3793794-hydroxy-3-methylbut-2-enyl diphosphate reductasePRO_0000128880Add
    BLAST

    Proteomic databases

    PaxDbiQ55643.

    Interactioni

    Protein-protein interaction databases

    IntActiQ55643. 4 interactions.
    STRINGi1148.slr0348.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 2923Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the IspH family.Curated

    Phylogenomic databases

    eggNOGiCOG0761.
    KOiK03527.
    OMAiDPDNLCF.
    OrthoDBiEOG6HF624.
    PhylomeDBiQ55643.

    Family and domain databases

    HAMAPiMF_00191. IspH.
    InterProiIPR003451. LytB.
    [Graphical view]
    PfamiPF02401. LYTB. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q55643-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY    50
    ETRQHFPGDR LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG 100
    DVVILPAFGA SVEEMQLLND RECTIVDTTC PWVSKVWNSV EKHKKKEHTS 150
    IIHGKYNHEE TIATSSFAGT YLIVLNMAEA QKVCDYILHG GDRQEFLDYF 200
    ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT LLQKYGPIEL 250
    NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA 300
    VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG 350
    ASTPDKVVEE VMKKILAIKE AQPVLEIAG 379
    Length:379
    Mass (Da):42,415
    Last modified:December 1, 2000 - v2
    Checksum:i4CD0167AF5D4957E
    GO

    Sequence cautioni

    The sequence BAA10159.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38915 Genomic DNA. Translation: AAB72119.1.
    BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
    PIRiS76307.
    RefSeqiNP_442089.1. NC_000911.1.
    YP_005652147.1. NC_017277.1.
    YP_007451966.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA10159; BAA10159; BAA10159.
    GeneIDi952940.
    KEGGisyn:slr0348.
    syy:SYNGTS_2194.
    syz:MYO_122140.
    PATRICi23841772. VBISynSp132158_2433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38915 Genomic DNA. Translation: AAB72119.1 .
    BA000022 Genomic DNA. Translation: BAA10159.1 . Different initiation.
    PIRi S76307.
    RefSeqi NP_442089.1. NC_000911.1.
    YP_005652147.1. NC_017277.1.
    YP_007451966.1. NC_020286.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q55643. 4 interactions.
    STRINGi 1148.slr0348.

    Proteomic databases

    PaxDbi Q55643.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA10159 ; BAA10159 ; BAA10159 .
    GeneIDi 952940.
    KEGGi syn:slr0348.
    syy:SYNGTS_2194.
    syz:MYO_122140.
    PATRICi 23841772. VBISynSp132158_2433.

    Phylogenomic databases

    eggNOGi COG0761.
    KOi K03527.
    OMAi DPDNLCF.
    OrthoDBi EOG6HF624.
    PhylomeDBi Q55643.

    Enzyme and pathway databases

    UniPathwayi UPA00056 ; UER00097 .
    UPA00059 ; UER00105 .

    Family and domain databases

    HAMAPi MF_00191. IspH.
    InterProi IPR003451. LytB.
    [Graphical view ]
    Pfami PF02401. LYTB. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00216. ispH_lytB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
      Cassier-Chauvat C., Poncelet M., Chauvat F.
      Gene 195:257-266(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
      Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
      DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27184 / PCC 6803 / N-1.
    3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.
    4. "Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis."
      Cunningham F.X. Jr., Lafond T.P., Gantt E.
      J. Bacteriol. 182:5841-5848(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PATHWAY.

    Entry informationi

    Entry nameiISPH_SYNY3
    AccessioniPrimary (citable) accession number: Q55643
    Secondary accession number(s): Q55066
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3