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Q55643

- ISPH_SYNY3

UniProt

Q55643 - ISPH_SYNY3

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Protein
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Gene
ispH, lytB, slr0348
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.UniRule annotation
Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.UniRule annotation

Cofactori

Binds 1 3Fe-4S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Iron-sulfur (3Fe-4S) By similarity
Binding sitei69 – 691Substrate By similarity
Binding sitei108 – 1081Substrate By similarity
Metal bindingi130 – 1301Iron-sulfur (3Fe-4S) By similarity
Binding sitei158 – 1581Substrate By similarity
Binding sitei223 – 2231Substrate By similarity
Metal bindingi261 – 2611Iron-sulfur (3Fe-4S) By similarity
Binding sitei352 – 3521Substrate By similarity

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dimethylallyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-HAMAP
  3. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC:1.17.1.2)
Gene namesi
Name:ispH
Synonyms:lytB
Ordered Locus Names:slr0348
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3793794-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation
PRO_0000128880Add
BLAST

Proteomic databases

PaxDbiQ55643.

Interactioni

Protein-protein interaction databases

IntActiQ55643. 4 interactions.
STRINGi1148.slr0348.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 2923Substrate binding By similarity

Sequence similaritiesi

Belongs to the IspH family.

Phylogenomic databases

eggNOGiCOG0761.
KOiK03527.
OMAiDPDNLCF.
OrthoDBiEOG6HF624.
PhylomeDBiQ55643.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55643-1 [UniParc]FASTAAdd to Basket

« Hide

MGVMNTEYQS HLIQEIRQNN YRLERGDVTI LLAEAFGFCW GVERAVAMAY    50
ETRQHFPGDR LWITNEIIHN PSVNQRLREM EVNFIDVVNG EKDFSGVAKG 100
DVVILPAFGA SVEEMQLLND RECTIVDTTC PWVSKVWNSV EKHKKKEHTS 150
IIHGKYNHEE TIATSSFAGT YLIVLNMAEA QKVCDYILHG GDRQEFLDYF 200
ANAHSAGFDP DQDLVRLGVA NQTTMLKSET EMIGKLFEKT LLQKYGPIEL 250
NNHFMSFNTI CDATQERQDA MFDLVEEDLS LMVVIGGFNS SNTTHLQEIA 300
VEHGIPSVHI DSGDRIGPGN RVEHKPLGKD LEVLEPWLPA GKITVGVTSG 350
ASTPDKVVEE VMKKILAIKE AQPVLEIAG 379
Length:379
Mass (Da):42,415
Last modified:December 1, 2000 - v2
Checksum:i4CD0167AF5D4957E
GO

Sequence cautioni

The sequence BAA10159.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38915 Genomic DNA. Translation: AAB72119.1.
BA000022 Genomic DNA. Translation: BAA10159.1. Different initiation.
PIRiS76307.
RefSeqiNP_442089.1. NC_000911.1.
YP_005652147.1. NC_017277.1.
YP_007451966.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10159; BAA10159; BAA10159.
GeneIDi952940.
KEGGisyn:slr0348.
syy:SYNGTS_2194.
syz:MYO_122140.
PATRICi23841772. VBISynSp132158_2433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38915 Genomic DNA. Translation: AAB72119.1 .
BA000022 Genomic DNA. Translation: BAA10159.1 . Different initiation.
PIRi S76307.
RefSeqi NP_442089.1. NC_000911.1.
YP_005652147.1. NC_017277.1.
YP_007451966.1. NC_020286.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q55643. 4 interactions.
STRINGi 1148.slr0348.

Proteomic databases

PaxDbi Q55643.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA10159 ; BAA10159 ; BAA10159 .
GeneIDi 952940.
KEGGi syn:slr0348.
syy:SYNGTS_2194.
syz:MYO_122140.
PATRICi 23841772. VBISynSp132158_2433.

Phylogenomic databases

eggNOGi COG0761.
KOi K03527.
OMAi DPDNLCF.
OrthoDBi EOG6HF624.
PhylomeDBi Q55643.

Enzyme and pathway databases

UniPathwayi UPA00056 ; UER00097 .
UPA00059 ; UER00105 .

Family and domain databases

HAMAPi MF_00191. IspH.
InterProi IPR003451. LytB.
[Graphical view ]
Pfami PF02401. LYTB. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00216. ispH_lytB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria."
    Cassier-Chauvat C., Poncelet M., Chauvat F.
    Gene 195:257-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  4. "Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis."
    Cunningham F.X. Jr., Lafond T.P., Gantt E.
    J. Bacteriol. 182:5841-5848(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PATHWAY.

Entry informationi

Entry nameiISPH_SYNY3
AccessioniPrimary (citable) accession number: Q55643
Secondary accession number(s): Q55066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

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