Q55638 (HYPF_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyltransferase hypF
EC=2.1.3.-

Alternative name(s):
Carbamoyl phosphate-converting enzyme hypF
[NiFe]-hydrogenase maturation factor hypF
Short name=Hydrogenase maturation protein hypF

Gene names

Name:	hypF
Ordered Locus Names:	sll0322

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of hypE yielding a protein-S-carboxamide By similarity.
Pathway	Protein modification; [NiFe] hydrogenase maturation.
Sequence similarities	Belongs to the carbamoyltransferase hypF family. Contains 1 acylphosphatase-like domain. Contains 1 YrdC-like domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein carbamoylation Inferred from electronic annotation. Source: InterPro
Molecular function	carboxyl- or carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Carbamoyltransferase hypF		PRO_0000071618
Regions
	Domain	4 – 90	87	Acylphosphatase-like
	Domain	200 – 385	186	YrdC-like
	Zinc finger	107 – 131	25	C4-type Potential
	Zinc finger	157 – 182	26	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55638 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FA9BA174172AB60D	FASTA	767	85,358
	10 20 30 40 50 60 MLKTVAIQVQ GRVQGVGFRP FVYTLAQEMG LNGWVNNSTQ GATVVITADE KAIADFTERL 70 80 90 100 110 120 TKTLPPPGLI EQLAVEQLPL ESFTNFTIRP SSDGPKTASI LPDLSTCSAC LTELFDPSDR 130 140 150 160 170 180 RYLYPFINCT HCGPRYTIIE ALPYDRCRTT MARFRQCTDC EREYKQPGDR RFHAQPNACP 190 200 210 220 230 240 RCGPQLAFWN RQGQVIAEAN EALNFAVDNL KVGNIIAIKG LGGFHLCCDA TDFEAVEKLR 250 260 270 280 290 300 LRKHRPDKPL AVMYGNLGQI VEHYQPNNLE VELLQSAAAP IVLLNKKKQL ILVENIAPGN 310 320 330 340 350 360 PRVGVMLAYT PLHHLLLKKL KKPMVATSGN LAGEQICIDN IDALTRLQNI ADGFLVHDRP 370 380 390 400 410 420 IVCPVDDSVV QIVAGKPLFL RRARGYAPQP ITLPKPTQKK LLAMGGHYKN TVAIAKQNQA 430 440 450 460 470 480 YVSQHLGDLN SAPTYQNFEE AIAHLSQLYD FSPQEIVADL HPDYFSHQYA ENQALPVTFV 490 500 510 520 530 540 QHHYAHILAV MAEHGVMEES VLGIAWDGTG YGMDGTIWGG EFLKITQGTW QRIAHLQPFH 550 560 570 580 590 600 LLGNQQAIKY PHRIALALLW PTFGDDFSAD SLGNWLNFNN GFKNKINSRL NQDLNNKNLR 610 620 630 640 650 660 QLWQRGQAPL TSSMGRLFDG IATLIGLINE VTFEGQAAIA LEAQIMPNLT EEYYPLTLNN 670 680 690 700 710 720 KEKKLAVDWR PLIKAITTED RSKTNLIATK FHNSLVNLII TIAQQQGIEK VALGGGCFQN 730 740 750 760 CYLLASTITA LKKAGFSPLW PRELPPNDGA ICMGQLLAKI QARQYIC

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10154.1.
PIR	S76302.
RefSeq	NP_442084.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55638.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55638. 4 interactions.
STRING	Q55638.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953323.
GenomeReviews	Gene locus sll0322 in contig BA000022_GR.
KEGG	syn:sll0322.
NMPDR	fig|1148.1.peg.2185.
PATRIC	23841762. VBISynSp132158_2428.
Phylogenomic databases
eggNOG	COG0068.
HOGENOM	HBG287964.
OMA	GGHLKNT.
PhylomeDB	Q55638.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0322-MONOMER.
Family and domain databases
InterPro	IPR001792. Acylphosphatase-like. IPR017968. Acylphosphatase_CS. IPR004421. Carbamoyltransferase_HypF. IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR006070. Sua5/yciO/yrdC_N. IPR011125. Znf_HypF. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K04656.
PANTHER	PTHR10029:SF4. PTHR10029:SF4. 1 hit.
Pfam	PF00708. Acylphosphatase. 1 hit. PF01300. Sua5_yciO_yrdC. 1 hit. PF07503. zf-HYPF. 2 hits. [Graphical view]
PIRSF	PIRSF006256. CMPcnvr_hdrg_mat. 1 hit.
SUPFAM	SSF54975. Acylphosphatase. 1 hit. SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00143. HypF. 1 hit.
PROSITE	PS00150. ACYLPHOSPHATASE_1. 1 hit. PS00151. ACYLPHOSPHATASE_2. False negative. PS51160. ACYLPHOSPHATASE_3. 1 hit. PS51163. YRDC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYPF_SYNY3

Accession

Primary (citable) accession number: Q55638

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program