Q55621 (PUR1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	sll0757

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	4Fe-4S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: UniProtKB-EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 28	28	By similarity		PRO_0000029273
	Chain	29 – 495	467	Amidophosphoribosyltransferase		PRO_0000029274
Regions
	Domain	29 – 254	226	Glutamine amidotransferase type-2
Sites
	Active site	29	1	For GATase activity By similarity
	Metal binding	270	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	317	1	Magnesium By similarity
	Metal binding	379	1	Magnesium By similarity
	Metal binding	380	1	Magnesium By similarity
	Metal binding	416	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	467	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	470	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55621 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 417DC5FB4B21207D	FASTA	495	53,670
	10 20 30 40 50 60 MFPPSSDLTE LNDGQPLSGH HADKPEEACG VFGIYAPEEA VAKLTYFGLY ALQHRGQESA 70 80 90 100 110 120 GIATFAGTTV HCHKDMGLVS QVFQESKLNE MVGTLAVGHT RYSTTGSSHR VNAQPAVLPT 130 140 150 160 170 180 RLGPLALAHN GNLVNTNQLR EALAERGCED FVTTTDSEMI AVAIANEVDK GKDWVEGTIA 190 200 210 220 230 240 ALTLCAGAYS LVIGTPEGII GVRDPHGIRP LVIGVLEEET PRYVLASETC ALDIIGATYV 250 260 270 280 290 300 RTVEAGELVH ITESGLVSHR LAESADRKLC VFEMIYFSRP DSVVNDESLY TYRMRIGKHL 310 320 330 340 350 360 AKESPVDADL VMGVPDSGIP AAIGFSQASG IPYAEGLIKN RYVGRTFIQP TQHMREHGIR 370 380 390 400 410 420 MKLNPLKDVL AGKRIIIVDD SIVRGTTSRK IVRALREAGA TEVHMRISSP PVTHPCFYGI 430 440 450 460 470 480 DTDSQDQLIA ARLTVAEIAE QIEVDSLAYL SQEGMLLCTG EDISHFCSAC FNGRYPITVP 490 DAVRRSKLML ENITA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10132.1.
PIR	S76280.
RefSeq	NP_442062.1. NC_000911.1. YP_005652121.1. NC_017277.1. YP_007451940.1. NC_020286.1.
3D structure databases
ProteinModelPortal	Q55621.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	1148.sll0757.
Protein family/group databases
MEROPS	C44.001.
Proteomic databases
PaxDb	Q55621.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10132; BAA10132; BAA10132.
GeneID	12253334. 14617610. 952767.
KEGG	syn:sll0757. syy:SYNGTS_2168. syz:MYO_121880.
PATRIC	23841716. VBISynSp132158_2405.
Phylogenomic databases
eggNOG	COG0034.
HOGENOM	HOG000033688.
KO	K00764.
OMA	CDACFTG.
OrthoDB	EOG6KT2Q1.
PhylomeDB	Q55621.
ProtClustDB	PRK07349.
Enzyme and pathway databases
UniPathway	UPA00074; UER00124.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_SYNY3

Accession

Primary (citable) accession number: Q55621

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program