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Q55621 (PUR1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:sll0757
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis ›

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2828 By similarity
PRO_0000029273
Chain29 – 495467Amidophosphoribosyltransferase
PRO_0000029274

Regions

Domain29 – 254226Glutamine amidotransferase type-2

Sites

Active site291For GATase activity By similarity
Metal binding2701Iron-sulfur (4Fe-4S) By similarity
Metal binding3171Magnesium By similarity
Metal binding3791Magnesium By similarity
Metal binding3801Magnesium By similarity
Metal binding4161Iron-sulfur (4Fe-4S) By similarity
Metal binding4671Iron-sulfur (4Fe-4S) By similarity
Metal binding4701Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55621 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 417DC5FB4B21207D

FASTA49553,670
        10         20         30         40         50         60 
MFPPSSDLTE LNDGQPLSGH HADKPEEACG VFGIYAPEEA VAKLTYFGLY ALQHRGQESA 

        70         80         90        100        110        120 
GIATFAGTTV HCHKDMGLVS QVFQESKLNE MVGTLAVGHT RYSTTGSSHR VNAQPAVLPT 

       130        140        150        160        170        180 
RLGPLALAHN GNLVNTNQLR EALAERGCED FVTTTDSEMI AVAIANEVDK GKDWVEGTIA 

       190        200        210        220        230        240 
ALTLCAGAYS LVIGTPEGII GVRDPHGIRP LVIGVLEEET PRYVLASETC ALDIIGATYV 

       250        260        270        280        290        300 
RTVEAGELVH ITESGLVSHR LAESADRKLC VFEMIYFSRP DSVVNDESLY TYRMRIGKHL 

       310        320        330        340        350        360 
AKESPVDADL VMGVPDSGIP AAIGFSQASG IPYAEGLIKN RYVGRTFIQP TQHMREHGIR 

       370        380        390        400        410        420 
MKLNPLKDVL AGKRIIIVDD SIVRGTTSRK IVRALREAGA TEVHMRISSP PVTHPCFYGI 

       430        440        450        460        470        480 
DTDSQDQLIA ARLTVAEIAE QIEVDSLAYL SQEGMLLCTG EDISHFCSAC FNGRYPITVP 

       490 
DAVRRSKLML ENITA

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000022 Genomic DNA. Translation: BAA10132.1.
PIRS76280.
RefSeqNP_442062.1. NC_000911.1.
YP_005652121.1. NC_017277.1.
YP_007451940.1. NC_020286.1.

3D structure databases

ProteinModelPortalQ55621.
ModBaseSearch...

Protein-protein interaction databases

STRING1148.sll0757.

Protein family/group databases

MEROPSC44.001.

Proteomic databases

PaxDbQ55621.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10132; BAA10132; BAA10132.
GeneID12253334.
14617610.
952767.
KEGGsyn:sll0757.
syy:SYNGTS_2168.
PATRIC23841716. VBISynSp132158_2405.

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMAGIPFELG.
ProtClustDBPRK07349.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_SYNY3
AccessionPrimary (citable) accession number: Q55621
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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