UniProtKB - Q55621 (PUR1_SYNY3)
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Protein
Amidophosphoribosyltransferase
Gene
purF
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Functioni
Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation
Catalytic activityi
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (purF)
- Phosphoribosylamine--glycine ligase (purD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 29 | NucleophileUniRule annotation | 1 | |
Metal bindingi | 270 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 317 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 379 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 380 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 416 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 467 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 470 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- amidophosphoribosyltransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: UniProtKB-KW
- nucleoside metabolic process Source: InterPro
- purine nucleobase biosynthetic process Source: InterPro
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00074; UER00124. |
Protein family/group databases
MEROPSi | C44.001. |
Names & Taxonomyi
Protein namesi | Recommended name: AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)Short name: ATaseUniRule annotation Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation Short name: GPATaseUniRule annotation |
Gene namesi | Name:purFUniRule annotation Ordered Locus Names:sll0757 |
Organismi | Synechocystis sp. (strain PCC 6803 / Kazusa) |
Taxonomic identifieri | 1111708 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Merismopediaceae › Synechocystis › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000029273 | 1 – 28 | By similarityAdd BLAST | 28 | |
ChainiPRO_0000029274 | 29 – 495 | AmidophosphoribosyltransferaseAdd BLAST | 467 |
Proteomic databases
PaxDbi | Q55621. |
PRIDEi | Q55621. |
Structurei
3D structure databases
ProteinModelPortali | Q55621. |
SMRi | Q55621. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 254 | Glutamine amidotransferase type-2UniRule annotationAdd BLAST | 226 |
Sequence similaritiesi
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
HOGENOMi | HOG000033688. |
InParanoidi | Q55621. |
KOi | K00764. |
OMAi | FRPLCLG. |
PhylomeDBi | Q55621. |
Family and domain databases
CDDi | cd00715. GPATase_N. 1 hit. cd06223. PRTases_typeI. 1 hit. |
Gene3Di | 3.60.20.10. 3 hits. |
HAMAPi | MF_01931. PurF. 1 hit. |
InterProi | View protein in InterPro IPR017932. GATase_2_dom. IPR029055. Ntn_hydrolases_N. IPR000836. PRibTrfase_dom. IPR029057. PRTase-like. IPR005854. PurF. IPR035584. PurF_N. |
Pfami | View protein in Pfam PF00156. Pribosyltran. 1 hit. |
PIRSFi | PIRSF000485. Amd_phspho_trans. 1 hit. |
SUPFAMi | SSF53271. SSF53271. 1 hit. SSF56235. SSF56235. 1 hit. |
TIGRFAMsi | TIGR01134. purF. 1 hit. |
PROSITEi | View protein in PROSITE PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q55621-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFPPSSDLTE LNDGQPLSGH HADKPEEACG VFGIYAPEEA VAKLTYFGLY
60 70 80 90 100
ALQHRGQESA GIATFAGTTV HCHKDMGLVS QVFQESKLNE MVGTLAVGHT
110 120 130 140 150
RYSTTGSSHR VNAQPAVLPT RLGPLALAHN GNLVNTNQLR EALAERGCED
160 170 180 190 200
FVTTTDSEMI AVAIANEVDK GKDWVEGTIA ALTLCAGAYS LVIGTPEGII
210 220 230 240 250
GVRDPHGIRP LVIGVLEEET PRYVLASETC ALDIIGATYV RTVEAGELVH
260 270 280 290 300
ITESGLVSHR LAESADRKLC VFEMIYFSRP DSVVNDESLY TYRMRIGKHL
310 320 330 340 350
AKESPVDADL VMGVPDSGIP AAIGFSQASG IPYAEGLIKN RYVGRTFIQP
360 370 380 390 400
TQHMREHGIR MKLNPLKDVL AGKRIIIVDD SIVRGTTSRK IVRALREAGA
410 420 430 440 450
TEVHMRISSP PVTHPCFYGI DTDSQDQLIA ARLTVAEIAE QIEVDSLAYL
460 470 480 490
SQEGMLLCTG EDISHFCSAC FNGRYPITVP DAVRRSKLML ENITA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000022 Genomic DNA. Translation: BAA10132.1. |
PIRi | S76280. |
Genome annotation databases
EnsemblBacteriai | BAA10132; BAA10132; BAA10132. |
KEGGi | syn:sll0757. |
Similar proteinsi
Entry informationi
Entry namei | PUR1_SYNY3 | |
Accessioni | Q55621Primary (citable) accession number: Q55621 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |