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Q55621

- PUR1_SYNY3

UniProt

Q55621 - PUR1_SYNY3

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei29 – 291For GATase activityBy similarity
    Metal bindingi270 – 2701Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi317 – 3171MagnesiumBy similarity
    Metal bindingi379 – 3791MagnesiumBy similarity
    Metal bindingi380 – 3801MagnesiumBy similarity
    Metal bindingi416 – 4161Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi467 – 4671Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi470 – 4701Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:sll0757
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2828By similarityPRO_0000029273Add
    BLAST
    Chaini29 – 495467AmidophosphoribosyltransferasePRO_0000029274Add
    BLAST

    Proteomic databases

    PaxDbiQ55621.

    Interactioni

    Protein-protein interaction databases

    STRINGi1148.sll0757.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 254226Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    KOiK00764.
    OMAiQHAVEDD.
    OrthoDBiEOG6KT2Q1.
    PhylomeDBiQ55621.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q55621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPPSSDLTE LNDGQPLSGH HADKPEEACG VFGIYAPEEA VAKLTYFGLY    50
    ALQHRGQESA GIATFAGTTV HCHKDMGLVS QVFQESKLNE MVGTLAVGHT 100
    RYSTTGSSHR VNAQPAVLPT RLGPLALAHN GNLVNTNQLR EALAERGCED 150
    FVTTTDSEMI AVAIANEVDK GKDWVEGTIA ALTLCAGAYS LVIGTPEGII 200
    GVRDPHGIRP LVIGVLEEET PRYVLASETC ALDIIGATYV RTVEAGELVH 250
    ITESGLVSHR LAESADRKLC VFEMIYFSRP DSVVNDESLY TYRMRIGKHL 300
    AKESPVDADL VMGVPDSGIP AAIGFSQASG IPYAEGLIKN RYVGRTFIQP 350
    TQHMREHGIR MKLNPLKDVL AGKRIIIVDD SIVRGTTSRK IVRALREAGA 400
    TEVHMRISSP PVTHPCFYGI DTDSQDQLIA ARLTVAEIAE QIEVDSLAYL 450
    SQEGMLLCTG EDISHFCSAC FNGRYPITVP DAVRRSKLML ENITA 495
    Length:495
    Mass (Da):53,670
    Last modified:November 1, 1996 - v1
    Checksum:i417DC5FB4B21207D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA10132.1.
    PIRiS76280.
    RefSeqiNP_442062.1. NC_000911.1.
    YP_005652121.1. NC_017277.1.
    YP_007451940.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA10132; BAA10132; BAA10132.
    GeneIDi952767.
    KEGGisyn:sll0757.
    syy:SYNGTS_2168.
    syz:MYO_121880.
    PATRICi23841716. VBISynSp132158_2405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA10132.1 .
    PIRi S76280.
    RefSeqi NP_442062.1. NC_000911.1.
    YP_005652121.1. NC_017277.1.
    YP_007451940.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali Q55621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 1148.sll0757.

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    PaxDbi Q55621.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA10132 ; BAA10132 ; BAA10132 .
    GeneIDi 952767.
    KEGGi syn:sll0757.
    syy:SYNGTS_2168.
    syz:MYO_121880.
    PATRICi 23841716. VBISynSp132158_2405.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    KOi K00764.
    OMAi QHAVEDD.
    OrthoDBi EOG6KT2Q1.
    PhylomeDBi Q55621.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
      Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
      DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27184 / PCC 6803 / N-1.
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiPUR1_SYNY3
    AccessioniPrimary (citable) accession number: Q55621
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3