Q55612 (LPXD_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	slr0776

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059706
Sites
	Active site	248	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55612 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A56979396B7864D6	FASTA	344	36,194
	10 20 30 40 50 60 MEFSQLVERL QTHIQCHSLE KFGDRNPTLG GVAALTEALT GQISYVDSAK YAPQMKTTQA 70 80 90 100 110 120 SALILPPDAK LQAEADAQGI AWCATAQPRL LFAAAIAVFY QPYRPPAGIH ATAVIDPSVH 130 140 150 160 170 180 CGEDVSIGPH VVIYPNVTLG DRVCIHGNVV IYPGVTIGND SVLHGNCTIH ERTQIGQGCV 190 200 210 220 230 240 IHSGAAIGAE GFGFVPTPEG WFKMEQSGQV VLEDGVEIGC NSAVDRPAVG ETRIGKNTKL 250 260 270 280 290 300 DNMVHVAHGC RIGEACALAG QVGLAGGVTI GNRVILAGQV GVADKSEIGD GAIASAQTGI 310 320 330 340 HGKVGPKEVV CGSPHMPHKL YLKASAIYKR LPEMYDTLKK LKKV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10120.1.
PIR	S76268.
RefSeq	NP_442050.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55612.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55612.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952720.
GenomeReviews	Gene locus slr0776 in contig BA000022_GR.
KEGG	syn:slr0776.
NMPDR	fig|1148.1.peg.2151.
PATRIC	23841686. VBISynSp132158_2391.
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	AKISTLF.
PhylomeDB	Q55612.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0776-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_SYNY3

Accession

Primary (citable) accession number: Q55612

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program