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Protein

1,4-alpha-glucan-branching enzyme

Gene

glgB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei338 – 3381NucleophileBy similarity
Active sitei391 – 3911Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. generation of precursor metabolites and energy Source: dictyBase
  2. glycogen biosynthetic process Source: UniProtKB
  3. glycogen metabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme (EC:2.4.1.18)
Alternative name(s):
Glycogen-branching enzyme
Gene namesi
Name:glgB
ORF Names:DDB_G0274105
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274105. glgB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6786781,4-alpha-glucan-branching enzymePRO_0000327602Add
BLAST

Proteomic databases

PRIDEiQ555Q9.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0214943.

Structurei

3D structure databases

ProteinModelPortaliQ555Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
InParanoidiQ555Q9.
KOiK00700.
OMAiWNSETHA.
PhylomeDBiQ555Q9.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q555Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYSKATDG TKVIHDDPWL EPYKEVIKRR HNQVKNTIQK LEESEGSLLK
60 70 80 90 100
FSQGYEYFGF NVTKDGVNYR EWLPSAHEVY LVGDFNQWNK TSHPLERDNY
110 120 130 140 150
GRWSIFIPNN SNGECAIPHG SKIKIYLKLA NGNFDYRIPA WIKRVEQTKE
160 170 180 190 200
NPVFDGVFWN PSKQYVFKNK SPMKPTELRI YEAHVGMSSE LPEISTYSKF
210 220 230 240 250
KDTVLPMVKE LGYNCIQLMA VMEHAYYASF GYQVTNFFAI SSRFGTPEEL
260 270 280 290 300
KEMIDKAHEM GLLVFLDVVH SHASKNVLDG LNQLDGTDHH YFHSGGRGNH
310 320 330 340 350
ELWDSRLFNY GNWEVMRFLL SNLRFYVDEY HFDGFRFDGV TSMIYTHHGL
360 370 380 390 400
SPACSYDDYF GGAVDEDALN YLTLANVMLH TLNPSIVTIA EEVTGLATLC
410 420 430 440 450
RPFSEGGGDF DYRLAMGIPD KWIELVKEKK DEDWNMGTIA HMLSNRRYKE
460 470 480 490 500
KNIAYAESHD QSLVGDKTLA FWLMDKEMYT NMSVTTEETP IIDRGMSLHK
510 520 530 540 550
MIRLITSSLG GDGYLNFMGN EFGHPEWVDF PREGNNNSLH HARRRWDLYR
560 570 580 590 600
NPLLRYKQLR DFDIAMNKAE QEFRWLSSDF AYISLKHEDD KIIVFERASL
610 620 630 640 650
IFIFNFHPSK SFSDYRIGSG VPGKFINVLD SDRKEFGGHV RIGKDNYHYT
660 670
EDKPWHDRKY SLLIYIPSRT CLVLKKVD
Length:678
Mass (Da):78,891
Last modified:May 24, 2005 - v1
Checksum:i878594DD748805D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL69945.1.
RefSeqiXP_644004.1. XM_638912.1.

Genome annotation databases

EnsemblProtistsiDDB0214943; DDB0214943; DDB_G0274105.
GeneIDi8619432.
KEGGiddi:DDB_G0274105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000012 Genomic DNA. Translation: EAL69945.1.
RefSeqiXP_644004.1. XM_638912.1.

3D structure databases

ProteinModelPortaliQ555Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0214943.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiQ555Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0214943; DDB0214943; DDB_G0274105.
GeneIDi8619432.
KEGGiddi:DDB_G0274105.

Organism-specific databases

dictyBaseiDDB_G0274105. glgB.

Phylogenomic databases

eggNOGiCOG0296.
InParanoidiQ555Q9.
KOiK00700.
OMAiWNSETHA.
PhylomeDBiQ555Q9.

Enzyme and pathway databases

UniPathwayiUPA00164.

Miscellaneous databases

PROiQ555Q9.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiGLGB_DICDI
AccessioniPrimary (citable) accession number: Q555Q9
Secondary accession number(s): Q8T189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.