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Protein

Phycobiliprotein ApcE

Gene

apcE

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is postulated to act both as terminal energy acceptor (by its phycobilin-like domains) and as a linker polypeptide (by its repeats and arms) that stabilizes the phycobilisome core architecture. Has intrinsic bilin lyase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei190 – 1901Phycocyanobilin chromophore (covalent; via 1 link)By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
  2. photosynthesis Source: UniProtKB-KW
  3. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bile pigment, Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Phycobiliprotein ApcE (EC:4.-.-.-)
Alternative name(s):
Phycobilisome LCM core-membrane linker polypeptide
Phycobilisome core-membrane linker phycobiliprotein ApcE
Gene namesi
Name:apcE
Ordered Locus Names:slr0335
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

  1. Cellular thylakoid membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  2. Note: Anchors the phycobilisome perpendicularly to the cytoplasmic surface of the thylakoid membrane.By similarity

GO - Cellular componenti

  1. phycobilisome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Phycobilisome, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 896896Phycobiliprotein ApcEPRO_0000403185Add
BLAST

Post-translational modificationi

Contains one covalently linked bilin chromophore. This protein autochromophorylates (By similarity).By similarity

Proteomic databases

PaxDbiQ55544.

Interactioni

Subunit structurei

Heterodimer of ApcF (a variant beta-allophycocyanin). Phycobilisomes of this organism are composed of a two cylinder core, from which six rods radiate. The core is mainly composed of allophycocyanin alpha and beta chains and of minor components (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP522314EBI-862826,EBI-862916

Protein-protein interaction databases

IntActiQ55544. 7 interactions.
STRINGi1148.slr0335.

Structurei

Secondary structure

1
896
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni255 – 2573Combined sources
Helixi275 – 29016Combined sources
Helixi296 – 2983Combined sources
Helixi300 – 31011Combined sources
Helixi316 – 32510Combined sources
Helixi327 – 3337Combined sources
Turni334 – 3363Combined sources
Helixi339 – 35113Combined sources
Helixi358 – 38225Combined sources
Helixi384 – 3896Combined sources
Turni390 – 3934Combined sources
Helixi732 – 74716Combined sources
Helixi753 – 7597Combined sources
Helixi761 – 7688Combined sources
Helixi774 – 7829Combined sources
Helixi785 – 7917Combined sources
Beta strandi793 – 7953Combined sources
Helixi797 – 80812Combined sources
Beta strandi809 – 8113Combined sources
Helixi816 – 82914Combined sources
Helixi831 – 8399Combined sources
Helixi842 – 8476Combined sources
Beta strandi850 – 8523Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L06NMR-A254-401[»]
3OHWX-ray2.70A/B711-858[»]
3OSJX-ray2.30A/B/C/D254-400[»]
ProteinModelPortaliQ55544.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ55544.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 427181PBS-linker 1PROSITE-ProRule annotationAdd
BLAST
Domaini508 – 684177PBS-linker 2PROSITE-ProRule annotationAdd
BLAST
Domaini703 – 881179PBS-linker 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phycobilisome linker protein family.PROSITE-ProRule annotation
Contains 3 PBS-linker domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG10800.
HOGENOMiHOG000276791.
InParanoidiQ55544.
KOiK02096.
OMAiQIFERDI.
OrthoDBiEOG6Q5NPN.
PhylomeDBiQ55544.

Family and domain databases

Gene3Di1.10.490.20. 2 hits.
InterProiIPR009050. Globin-like.
IPR001297. PBS_linker_dom.
IPR012128. Phycobilisome_asu/bsu.
[Graphical view]
PfamiPF00427. PBS_linker_poly. 3 hits.
PF00502. Phycobilisome. 2 hits.
[Graphical view]
ProDomiPD000852. PBS_linker_poly. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46458. SSF46458. 2 hits.
PROSITEiPS51445. PBS_LINKER. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKASGGSS LARPQLYQTV PVSAISQAEQ QDRFLEGSEL NELTAYFQSG
60 70 80 90 100
ALRLEIAETL TQNADLIVSR AANRIFTGGS PLSYLEKPVE RQPALVGASS
110 120 130 140 150
DSRNGSVTYA ESNGSGGLFG GLRSVFSSTG PIPPGFRPIN IARYGPSNMQ
160 170 180 190 200
KSLRDMSWFL RYTTYAIVAG DPNIIVVNTR GLKEVIENAC SIDATIVAIQ
210 220 230 240 250
EMRAASADYF RNNAQAKEIV LQYFDILLSE FKAPTPANKV RQGPSNDIQG
260 270 280 290 300
LELPQSYFNA AAKRQKYAMK PGLSALEKNA VIKAAYRQIF ERDITKAYSQ
310 320 330 340 350
SISYLESQVR NGDISMKEFV RRLAKSPLYR KQFFEPFINS RALELAFRHI
360 370 380 390 400
LGRGPSSREE VQKYFSIVSS GGLPALVDAL VDSQEYADYF GEETVPYLRG
410 420 430 440 450
LGVEAQECRN WGMQQDLFSY SAPFRKVPQF ITTFAQYDRP LPDQHVYGSG
460 470 480 490 500
NDPLEIQFGA IFPKETRNPS KRPAPFNKDT KRILIHRGPA VNNQVGNPSA
510 520 530 540 550
VGEFPGSLGA KVFRLNGGLP GAKVGKNTGT SVKFGESSTQ ALIRAAYRQV
560 570 580 590 600
FGRDLYEGQR LSVAEIQLEN GDISVREFIK RLAKSELFLK LYWAPHYVCK
610 620 630 640 650
AIEYMHRRLL GRPTYGRQEM NQYFDIASKQ GFYAVVEAMI DSKEYSDAFG
660 670 680 690 700
EDTVPYERYL TPGGLQMRSA RVGSLREDIG QRVDKEVTPR FVELGQVSAI
710 720 730 740 750
RTEPEIAYRS NQGVTRQRQQ TKVFKLVSTY DKVAVKNAIR AAYRQVFERD
760 770 780 790 800
LEPYIINSEF TALESKLSNN EINVKEFIEG LGTSELYMKE FYAPYPNTKV
810 820 830 840 850
IEMGTKHFLG RAPLNQKEIQ QYNQILASQG LKAFIGAMVN GMEYLQTFGE
860 870 880 890
DTVPYRRFPT LPAANFPNTE RLYNKLTKQD KELVVPSFTP VVKVGG
Length:896
Mass (Da):100,296
Last modified:November 1, 1996 - v1
Checksum:i05D4358C5F0BCD8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10042.1.
PIRiS76064.
RefSeqiNP_441972.1. NC_000911.1.
YP_005652032.1. NC_017277.1.
YP_007451852.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10042; BAA10042; BAA10042.
KEGGisyn:slr0335.
PATRICi23841504. VBISynSp132158_2300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10042.1.
PIRiS76064.
RefSeqiNP_441972.1. NC_000911.1.
YP_005652032.1. NC_017277.1.
YP_007451852.1. NC_020286.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L06NMR-A254-401[»]
3OHWX-ray2.70A/B711-858[»]
3OSJX-ray2.30A/B/C/D254-400[»]
ProteinModelPortaliQ55544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55544. 7 interactions.
STRINGi1148.slr0335.

Proteomic databases

PaxDbiQ55544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10042; BAA10042; BAA10042.
KEGGisyn:slr0335.
PATRICi23841504. VBISynSp132158_2300.

Phylogenomic databases

eggNOGiNOG10800.
HOGENOMiHOG000276791.
InParanoidiQ55544.
KOiK02096.
OMAiQIFERDI.
OrthoDBiEOG6Q5NPN.
PhylomeDBiQ55544.

Miscellaneous databases

EvolutionaryTraceiQ55544.

Family and domain databases

Gene3Di1.10.490.20. 2 hits.
InterProiIPR009050. Globin-like.
IPR001297. PBS_linker_dom.
IPR012128. Phycobilisome_asu/bsu.
[Graphical view]
PfamiPF00427. PBS_linker_poly. 3 hits.
PF00502. Phycobilisome. 2 hits.
[Graphical view]
ProDomiPD000852. PBS_linker_poly. 3 hits.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF46458. SSF46458. 2 hits.
PROSITEiPS51445. PBS_LINKER. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiAPCE_SYNY3
AccessioniPrimary (citable) accession number: Q55544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.