Reviewed, UniProtKB/Swiss-Prot Q55522 (SYV_SYNY3)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	slr0557

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862103,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000106237
Regions
	Coiled coil	845 – 909	65	Potential
	Motif	46 – 56	11	"HIGH" region HAMAP MF_02004
	Motif	539 – 543	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	542	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55522-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D909BCD889BF42E	FASTA	910	102,737
	10 20 30 40 50 60 MTTSLPPQYE PTVTEAKWQT AWEESHAFKA DPDRPGEPYC VVIPPPNVTG SLHMGHAFES 70 80 90 100 110 120 SLIDTLVRYH RMRGDNTLWL PGTDHASIAV QTILERQLKA EGKTRDDLGR EKFLERAWQW 130 140 150 160 170 180 KAESGSTIVN QLRRLGVSVD WTRERFTMDE GLSQAVKTAF IKLYEEGLIY RGNYLVNWCP 190 200 210 220 230 240 ASQSAVSDLE VENQEVDGHL WYFRYPLTDG SGELVVATTR PETMLGDTGV AVNPHDERYA 250 260 270 280 290 300 AMVGKTITLP LVNREIPIVA DELVDPEFGT GCVKVTPAHD PNDFVMGQRH NLPFINLLNK 310 320 330 340 350 360 DGSLNENGGD FAGQDRFEAR KNVVQALEAQ GFLVKIEPYR HSVPYGDRGK VPVEPLLSTQ 370 380 390 400 410 420 WFVKIESLAQ NALACLDEDN SPNFVPERWG KVYRDWLVKL KDWCISRQLW WGHQIPAWYV 430 440 450 460 470 480 ISETNGAITD HTPFIVAYDE AEALAKAKAE YGPTVQLQQD PDVLDTWFSS GLWPFSTMGW 490 500 510 520 530 540 PEQTDDLAKY YPTSTLVTGF DIIFFWVARM TMMAGHFTGQ IPFKDVYIHG LVRDENGKKM 550 560 570 580 590 600 SKSANNGIDP LLLINKYGTD ALRYTLIREV AGAGQDISLQ YDRQKDESES VEASRNFANK 610 620 630 640 650 660 LWNAARFVMM NLDGQTPQQL GLAPGEDLEL ADRWILSRLN QVIQQTREQI EDYGLGEAAK 670 680 690 700 710 720 GLYEFIWGDF CDWYIELAKP RLWNKEGGDV GTQRQLVARQ VLAHTLDSII KLLHPFMPHI 730 740 750 760 770 780 TEELWQTLHQ AEGQFLALQA YPTVNQSLVD PALETQFALL IETLRTIRNL RAEAGIKPGA 790 800 810 820 830 840 MVTVILQSEN DQERQTLQLG ETYIRDIGKV ENLQVVSQLP PEQTQAIAGV VDTIQVLIPL 850 860 870 880 890 900 SGLVDLDILR NKIQKTLDKV TKEYESIEKR LSNPGFVNKA PEEVIAGAKE SLNAAAVQRQ 910 MLQERLKMLG

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10881.1.
PIR	S76034.
RefSeq	NP_442810.1.
3D structure databases
HSSP	HSSP built from PDB template 1IVS based on UniProtKB P96142.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55522. 2 interactions.
Genome annotation databases
GeneID	952772.
GenomeReviews	Gene locus slr0557 in contig BA000022_GR.
KEGG	syn:slr0557.
NMPDR	fig|1148.1.peg.2911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55522.
OMA	Q55522. TDQWYVS.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0557-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR011321. Val-tRNA_synth_Ia_C. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.287.380. Val-tRNA_synth_Ia_C. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_SYNY3

Accession

Primary (citable) accession number: Q55522

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)