Reviewed, UniProtKB/Swiss-Prot Q55512 (DHAS_SYNY3)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	slr0549

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Aspartate-semialdehyde dehydrogenase		PRO_0000141393
Sites
	Active site	72	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55512-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3335A9BB4A6FFB7B	FASTA	280	30,605
	10 20 30 40 50 60 MDGSAFKGCD LVLASAGGST SKRWAEEITK AGAVMVDNSS AFRMVPEVPL VVPEINPEAA 70 80 90 100 110 120 QNHQGIIANP NCTTILMGVA IYPLHQLQPI KRIVVATYQS ASGAGAMAME EVKHQSRDIL 130 140 150 160 170 180 EGKIPQAEIL PYPLAFNLFP HNSPITANHY CEEEMKMVQE TRKIFAAEDI RITATCVRVP 190 200 210 220 230 240 VLRAHSEAVN LEFATPFPVE LAKTAIAKAP GVKLVEDWQK NYFPMPMDAT GQDDVLVGRI 250 260 270 280 RQDISHPNGL DLWLCGDQIR KGAALNAVQI AELLVERGWL

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10869.1.
PIR	S76022.
RefSeq	NP_442798.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55512. 2 interactions.
Genome annotation databases
GeneID	952082.
GenomeReviews	Gene locus slr0549 in contig BA000022_GR.
KEGG	syn:slr0549.
NMPDR	fig|1148.1.peg.2899.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55512.
OMA	Q55512. LELWLCG.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0549-MON.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PROSITE	PS01103. ASD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_SYNY3

Accession

Primary (citable) accession number: Q55512

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)