Reviewed, UniProtKB/Swiss-Prot Q554E2 (ALG8_DICDI)

Names and origin

Protein names

Recommended name:
    Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 8 homolog

Gene names

Name:	alg8
ORF Names:	DDB_G0275261

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.
Sequence caution	The sequence AAO52348.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAO52349.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000327820
Regions
	Transmembrane	41 – 61	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	205 – 225	21	Potential
	Transmembrane	234 – 254	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	314 – 334	21	Potential
	Transmembrane	415 – 435	21	Potential
	Transmembrane	453 – 473	21	Potential
	Transmembrane	484 – 504	21	Potential
	Transmembrane	509 – 529	21	Potential
	Transmembrane	567 – 587	21	Potential
	Transmembrane	595 – 615	21	Potential
	Compositional bias	5 – 14	10	Poly-Asn
	Compositional bias	160 – 196	37	Poly-Asn
	Compositional bias	298 – 302	5	Poly-Gln
	Compositional bias	533 – 549	17	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q554E2-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C86A19157C469398	FASTA	625	72,999
	10 20 30 40 50 60 MIKKNNNSNN NNNNPIIIKD GTNCFFKEIK DVLKFSRNWN LIILVSTIKL LLIPSYLSTD 70 80 90 100 110 120 FEVHRNWLAI TSSLPISKWY FENTSEWTLD YPPFFGWFEY FLSKFAYYID SEMLVIDNLG 130 140 150 160 170 180 YKSTSTILFQ RFSVIISDSL FILSTLLLSN YIYNIIIKKN NNKNNNNNNN NNNNNNNNNN 190 200 210 220 230 240 NNNNNNNNNN NNNNNNLHWY HDKSFIISSI IILNPGLLMV DHIHFQYNGF LKGILILSIY 250 260 270 280 290 300 FLVRGNILIG SILFSVLLNF KHIYMYMAPA FFVYLLKYYC LKSNLNDNTT SKVNHSKQQQ 310 320 330 340 350 360 QQEFTIFGIK IFNLIKLGIS VLSIFLISLG PFFYMGQIQQ LISRLFPFGR GLSHAYWAPN 370 380 390 400 410 420 FWSIYNFLDR VLLFNGLYKK IPFFKNFIIP DQVTGNLTSG LVGSETQSHI LLPKITPQIT 430 440 450 460 470 480 LLITILFLIP SIYSILKSKS WKHFILGICQ SSFTFFMFGW HVHEKAIIMI TIPMGFLCLV 490 500 510 520 530 540 NNKFSKLYFL LSTIGHYSLF PLLFQVEEIP TRILILVTYT LLVYLSFISS SSNNNNNNNN 550 560 570 580 590 600 NNNNSNNNNS KQRKCSFSLG LYWFEKFYLF GIILLEIFNV FIHPVFLAHI ERFSFISLMI 610 620 TSVYTSVGII YCYLFTFKLI FKNEY

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116305 Genomic DNA. Translation: AAO52348.1. Sequence problems. AC116305 Genomic DNA. Translation: AAO52349.1. Sequence problems. AAFI02000013 Genomic DNA. Translation: EAL69911.1.
RefSeq	XP_643769.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3394960.
KEGG	ddi:DDB_0231452.
Organism-specific databases
dictyBase	DDB_G0275261. alg8.
Phylogenomic databases
OMA	Q554E2. ARERRYV.
Family and domain databases
InterPro	IPR004856. Glycosyltransferase_ALG6/ALG8. [Graphical view]
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG8_DICDI

Accession

Primary (citable) accession number: Q554E2
Secondary accession number(s): Q869W2, Q86I13

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)