Q554D9 (SYNC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase, cytoplasmic
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS1
ORF Names:	DDB_G0275263

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	phagocytic vesicle Inferred from direct assay Ref.3. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	Asparagine--tRNA ligase, cytoplasmic		PRO_0000341691

Sequences

	Sequence		Length	Mass (Da)
	Q554D9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 2EA4FC1932E1C1AB	FASTA	653	74,555
	10 20 30 40 50 60 MSTEEDQKLF LSIGLDEKRA KDTLKNKDLT ALLKTIIEEA SVSKGCDKSI GNLLYSLATC 70 80 90 100 110 120 QQVNSPIRSN VSKAIGEEKI KTTIQFQAAT QYLKDNKEFN QDTFNDFCGV GVVITPEQIQ 130 140 150 160 170 180 KAIDDLFTKK ATEISEKKWH IPIGDLLNPL KESLKWADLK EVKIMLDKKL ETTLGPKVVE 190 200 210 220 230 240 AKEKKVAATT TAPVKKIEEQ LAAITLKQDA SLPTPKRLKI RECSPKFANQ RVEVHAWAHH 250 260 270 280 290 300 VRNQKKIVFL ELRDGTGFLQ CVLSGDLVHP SIIDKLLREA TVKIVGTLVI PPADKKCPGG 310 320 330 340 350 360 VELQADYWEL LGPSNADLEG VINQESNVDH LFDQRHIMMR GTRASSIMKI RSLCLFAFRQ 370 380 390 400 410 420 HFFENHYMEV TPPTLVNTYC EGGSELFTVD YFGKPAYLTQ SSQLYLETML PVLGDNFTIT 430 440 450 460 470 480 QSYRAEKART RRHLTEFLHL EAECPFITYE ELQDRIEFLV VDVCEKLFKM DPELILSVNP 490 500 510 520 530 540 DFKVPKRPFM RMNYSDAIEY CKKNGIQKKL EDGTEVDFEF GDDIPEAQER RMNDQIGEPI 550 560 570 580 590 600 FLCRFPAEMK AFYMARCPED RTLTESLDLL MPGVGEIVGG SMRISDFNEL VAAYKKAGLD 610 620 630 640 650 TDEYYWFTDQ RKYGTTQHGG YGLGVERFLT WMLKEEHIRN VCVYQRTKDR ITP

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69912.1.
RefSeq	XP_643772.1. XM_638680.1.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
ProteinModelPortal	Q554D9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q554D9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231333; DDB0231333; DDB_G0275263.
GeneID	8619817.
GenomeReviews	Gene locus asnS1 in contig CM000151_GR.
KEGG	ddi:DDB_G0275263.
Organism-specific databases
dictyBase	DDB_G0275263. asnS1.
Phylogenomic databases
eggNOG	KOG0555.
GeneTree	EPrGT00050000000242.
HOGENOM	HBG745843.
OMA	DQPERAM.
PhylomeDB	Q554D9.
ProtClustDB	CLSZ2430982.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNC_DICDI

Accession

Primary (citable) accession number: Q554D9
Secondary accession number(s): Q869W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)