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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Carbamoyl phosphateBy similarity
Binding sitei80 – 801Carbamoyl phosphateBy similarity
Binding sitei104 – 1041Carbamoyl phosphateBy similarity
Binding sitei162 – 1621OrnithineBy similarity
Binding sitei225 – 2251OrnithineBy similarity
Binding sitei275 – 2751Carbamoyl phosphateBy similarity
Binding sitei293 – 2931Carbamoyl phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:sll0902
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Ornithine carbamoyltransferasePRO_0000113048Add
BLAST

Proteomic databases

PRIDEiQ55497.

Interactioni

Protein-protein interaction databases

IntActiQ55497. 2 interactions.

Structurei

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Important for structural integrityBy similarity
Sitei144 – 1441Important for structural integrityBy similarity

3D structure databases

ProteinModelPortaliQ55497.
SMRiQ55497. Positions 8-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 575Carbamoyl phosphate bindingBy similarity
Regioni131 – 1344Carbamoyl phosphate bindingBy similarity
Regioni229 – 2302Ornithine bindingBy similarity
Regioni264 – 2674Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

HOGENOMiHOG000022686.
InParanoidiQ55497.
KOiK00611.
OMAiEHPCQIL.
PhylomeDBiQ55497.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKALAGRD LLAIADLTIE EMKSLLQLAA DLKSGVLKPH CRKILGLLFY
60 70 80 90 100
KASTRTRVSF TAAMYQLGGQ VLDLNPSVTQ VGRGEPIQDT ARVLDRYIDI
110 120 130 140 150
LAVRTFKQTD LQTFADHAKM PIINALSDLE HPCQILADLQ TIKECFGKLE
160 170 180 190 200
GLTVTYLGDG NNVAHSLILG GVMMGMTVRV ATPKNYEPLA EIVQQAQQIA
210 220 230 240 250
APGGKVELTD DPKAAAQGSH ILYTDVWASM GQEDLADSRI PIFQPYQINQ
260 270 280 290 300
ELLALADPEA IVLHCLPAHR GEEITDAVME GPQSRLWDQA ENRMHAQKAL

MVALLGLV
Length:308
Mass (Da):33,616
Last modified:November 1, 1996 - v1
Checksum:iC6CD2E4998592CFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10847.1.
PIRiS76000.

Genome annotation databases

EnsemblBacteriaiBAA10847; BAA10847; BAA10847.
KEGGisyn:sll0902.
PATRICi23843314. VBISynSp132158_3193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10847.1.
PIRiS76000.

3D structure databases

ProteinModelPortaliQ55497.
SMRiQ55497. Positions 8-306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55497. 2 interactions.

Proteomic databases

PRIDEiQ55497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10847; BAA10847; BAA10847.
KEGGisyn:sll0902.
PATRICi23843314. VBISynSp132158_3193.

Phylogenomic databases

HOGENOMiHOG000022686.
InParanoidiQ55497.
KOiK00611.
OMAiEHPCQIL.
PhylomeDBiQ55497.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_SYNY3
AccessioniPrimary (citable) accession number: Q55497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.