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Protein

Magnesium-protoporphyrin O-methyltransferase

Gene

chlM

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor.

Catalytic activityi

S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester.PROSITE-ProRule annotation

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ55467.
UniPathwayiUPA00670.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium-protoporphyrin O-methyltransferase (EC:2.1.1.11)
Alternative name(s):
Magnesium-protoporphyrin IX methyltransferase
Gene namesi
Name:chlM
Ordered Locus Names:slr0525
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Magnesium-protoporphyrin O-methyltransferasePRO_0000204421Add
BLAST

Proteomic databases

PaxDbiQ55467.

Interactioni

Protein-protein interaction databases

IntActiQ55467. 4 interactions.
STRINGi1148.slr0525.

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 169Combined sources
Helixi19 – 279Combined sources
Beta strandi29 – 313Combined sources
Helixi35 – 5824Combined sources
Beta strandi65 – 695Combined sources
Turni72 – 765Combined sources
Helixi77 – 826Combined sources
Beta strandi86 – 927Combined sources
Helixi94 – 10815Combined sources
Beta strandi115 – 1184Combined sources
Helixi121 – 1233Combined sources
Beta strandi128 – 1336Combined sources
Helixi137 – 1393Combined sources
Helixi142 – 15514Combined sources
Beta strandi156 – 1649Combined sources
Helixi169 – 17810Combined sources
Helixi195 – 20410Combined sources
Beta strandi207 – 21610Combined sources
Beta strandi221 – 2299Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QDJX-ray1.60A1-230[»]
4QDKX-ray1.70A/B1-230[»]
ProteinModelPortaliQ55467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Magnesium protoporphyrin O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2227.
HOGENOMiHOG000154343.
InParanoidiQ55467.
KOiK03428.
OMAiLDVFIHY.
OrthoDBiEOG6JMMSP.
PhylomeDBiQ55467.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF07109. Mg-por_mtran_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02021. BchM-ChlM. 1 hit.
PROSITEiPS51556. SAM_MT_MG_PIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNAALDDKT IVRDYFNSTG FDRWRRIYGD GQVNFVQKDI RVGHQQTVDS
60 70 80 90 100
VVAWLVADGN LPGLLVCDAG CGVGSLSIPL AQAGALVYGS DISEKMVGEA
110 120 130 140 150
QQKAQEVLAY GNQPTFMTQD LAQLGGKYDT VICLDVLIHY PTEEASAMIS
160 170 180 190 200
HLASLADRRL ILSFAPKTLG LTVLKKIGGL FPGPSKTTRA YQHKEADIRK
210 220 230
ILGDNGFSIA RTGMTSTRFY YSRILEAVRS
Length:230
Mass (Da):25,081
Last modified:November 1, 1996 - v1
Checksum:i166D72CF3DD178A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901A → V in AAA85380 (PubMed:8704138).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47126 Genomic DNA. Translation: AAA85380.1.
BA000022 Genomic DNA. Translation: BAA10812.1.
PIRiS71781.
RefSeqiYP_005652802.1. NC_017277.1.
YP_007452617.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10812; BAA10812; BAA10812.
KEGGisyn:slr0525.
PATRICi23843234. VBISynSp132158_3155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47126 Genomic DNA. Translation: AAA85380.1.
BA000022 Genomic DNA. Translation: BAA10812.1.
PIRiS71781.
RefSeqiYP_005652802.1. NC_017277.1.
YP_007452617.1. NC_020286.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QDJX-ray1.60A1-230[»]
4QDKX-ray1.70A/B1-230[»]
ProteinModelPortaliQ55467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55467. 4 interactions.
STRINGi1148.slr0525.

Proteomic databases

PaxDbiQ55467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10812; BAA10812; BAA10812.
KEGGisyn:slr0525.
PATRICi23843234. VBISynSp132158_3155.

Phylogenomic databases

eggNOGiCOG2227.
HOGENOMiHOG000154343.
InParanoidiQ55467.
KOiK03428.
OMAiLDVFIHY.
OrthoDBiEOG6JMMSP.
PhylomeDBiQ55467.

Enzyme and pathway databases

UniPathwayiUPA00670.
SABIO-RKQ55467.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR010251. Mg_prot_MeTrfase.
IPR010940. Mg_prot_MeTrfase_C.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF07109. Mg-por_mtran_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02021. BchM-ChlM. 1 hit.
PROSITEiPS51556. SAM_MT_MG_PIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus."
    Smith C.A., Suzuki J.Y., Bauer C.E.
    Plant Mol. Biol. 30:1307-1314(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiCHLM_SYNY3
AccessioniPrimary (citable) accession number: Q55467
Secondary accession number(s): Q55344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.