Reviewed, UniProtKB/Swiss-Prot Q55451 (CBIX_SYNY3)

Names and origin

Protein names

Recommended name:
    Sirohydrochlorin cobaltochelatase
    EC=4.99.1.3
Alternative name(s):
    CbiXL

Gene names

Name:	cbiX
Ordered Locus Names:	sll0037

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis.
Catalytic activity	Cobalt-sirohydrochlorin + 2 H+ = sirohydrochlorin + Co2+.
Cofactor	Binds 1 4Fe-4S cluster per dimer Probable.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the cbiX family. CbiXL subfamily.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	4Fe-4S Cobalt Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sirohydrochlorin cobaltochelatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Sirohydrochlorin cobaltochelatase		PRO_0000150352
Regions
	Compositional bias	302 – 309	8	Poly-His
Sites
	Metal binding	282	1	Iron-sulfur (4Fe-4S); shared with dimeric partner Probable
	Metal binding	285	1	Iron-sulfur (4Fe-4S); shared with dimeric partner Probable

Sequences

	Sequence		Length	Mass (Da)
	Q55451-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 70981BD77DBAC4B9	FASTA	336	38,340
	10 20 30 40 50 60 MTLTSVPAPV SLFPELELPP LPYHRPLLMI GHGTRDEDGR QTFLDFVAQY QALDHSRPVI 70 80 90 100 110 120 PCFLELTEPN IQAGVQQCVD QGFEEISALP ILLFAARHNK FDVTNELDRS RQAHPQINFF 130 140 150 160 170 180 YGRHFGITPA ILDLWKARLN QLDSPEANPQ GIDRQDTVLL FVGRGSSDPD ANGDVYKMAR 190 200 210 220 230 240 MLWEGSGYQT VETCFIGISH PRLEEGFRRA RLYQPKRIIV LPYFLFMGAL VKKIFTITEE 250 260 270 280 290 300 QRATFPEIEI QSLSEMGIQP ELLALVRERE IETQLGQVAM NCEACKFRLA FKNQGHGHDH 310 320 330 GHGHHHHGHD HGHSHGEWVD TYIEPTAYHE KIWQAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Characterization of the cobaltochelatase CbiXL: evidence for a 4Fe-4S center housed within an MXCXXC motif." Leech H.K., Raux E., McLean K.J., Munro A.W., Robinson N.J., Borrelly G.P.M., Malten M., Jahn D., Rigby S.E.J., Heathcote P., Warren M.J. J. Biol. Chem. 278:41900-41907(2003) [PubMed: 12917443] [Abstract] Cited for: CHARACTERIZATION OF IRON-SULFUR CLUSTER.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10794.1. Different initiation.
PIR	S75947.
RefSeq	NP_442723.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55451.
Genome annotation databases
GeneID	952151.
GenomeReviews	Gene locus sll0037 in contig BA000022_GR.
KEGG	syn:sll0037.
NMPDR	fig|1148.1.peg.2824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55451.
OMA	DPDANSN
Enzyme and pathway databases
BioCyc	SSP1148:SLL0037-MON.
Family and domain databases
InterPro	IPR002762. Cbl_biosynth_CbiX. [Graphical view]
Pfam	PF01903. CbiX. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBIX_SYNY3

Accession

Primary (citable) accession number: Q55451

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)