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Q55434

- PHY2_SYNY3

UniProt

Q55434 - PHY2_SYNY3

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Protein
Phytochrome-like protein cph2
Gene
cph2, sll0821
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Photoreceptor which exists in two forms that are reversibly interconvertible by light: the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291Tetrapyrrole chromophore 1 (covalent; via 1 link) Inferred
Binding sitei1022 – 10221Tetrapyrrole chromophore 2 (covalent; via 1 link) Reviewed prediction

GO - Molecular functioni

  1. phosphorus-oxygen lyase activity Source: InterPro
  2. red or far-red light photoreceptor activity Source: UniProtKB

GO - Biological processi

  1. cyclic nucleotide biosynthetic process Source: InterPro
  2. detection of visible light Source: InterPro
  3. intracellular signal transduction Source: InterPro
  4. protein-chromophore linkage Source: UniProtKB
  5. red, far-red light phototransduction Source: UniProtKB
  6. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Sensory transduction

Keywords - Ligandi

Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Phytochrome-like protein cph2
Alternative name(s):
Bacteriophytochrome cph2
Gene namesi
Name:cph2
Ordered Locus Names:sll0821
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi129 – 1291C → S: Holoprotein exhibits no photochromic activity. 1 Publication
Mutagenesisi130 – 1301H → F: Chromophore ligating activity (in vitro) is 30-40% lower than wild-type. 1 Publication
Mutagenesisi130 – 1301H → Q: Chromophore ligating activity (in vitro) is about 10% more efficient than wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12761276Phytochrome-like protein cph2
PRO_0000172002Add
BLAST

Post-translational modificationi

Contains two covalently linked tetrapyrrole chromophores.

Interactioni

Protein-protein interaction databases

DIPiDIP-48801N.
IntActiQ55434. 11 interactions.
STRINGi1148.sll0821.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1812
Helixi24 – 3916
Beta strandi42 – 498
Beta strandi55 – 628
Turni64 – 663
Helixi77 – 793
Helixi82 – 898
Beta strandi93 – 986
Helixi99 – 1013
Beta strandi103 – 1108
Helixi128 – 1369
Beta strandi140 – 14910
Beta strandi152 – 16312
Helixi169 – 21547
Helixi219 – 23214
Beta strandi236 – 2427
Beta strandi252 – 2576
Helixi263 – 2664
Helixi269 – 2757
Beta strandi313 – 3164
Helixi317 – 3204
Turni331 – 3333
Helixi334 – 3363
Beta strandi338 – 34710
Beta strandi352 – 36110
Beta strandi363 – 3719
Beta strandi388 – 3958
Helixi401 – 41818

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BWIX-ray2.60A/B2-424[»]
ProteinModelPortaliQ55434.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 186164GAF 1
Add
BLAST
Domaini461 – 598138GGDEF 1
Add
BLAST
Domaini607 – 861255EAL
Add
BLAST
Domaini939 – 1080142GAF 2
Add
BLAST
Domaini1130 – 1266137GGDEF 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 197197Bilin lyase domain 1
Add
BLAST
Regioni939 – 1075137Bilin lyase domain 2
Add
BLAST

Domaini

Both bilin lyase domains bind with the bilin tetrapyrrole chromophore precursor. The domain 1 shows red, far-red light photoreversibility. The domain 2 is photochemically inactive.1 Publication

Sequence similaritiesi

Belongs to the phytochrome family.
Contains 1 EAL domain.
Contains 2 GAF domains.
Contains 2 GGDEF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2203.
KOiK02488.
OMAiAIHQGEL.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di3.20.20.450. 1 hit.
3.30.450.40. 4 hits.
InterProiIPR001054. A/G_cyclase.
IPR001633. Diguanylate_PEstase_EAL_dom.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR000160. GGDEF_dom.
IPR016132. Phyto_chromo_attachment.
IPR001294. Phytochrome.
IPR013515. Phytochrome_cen-reg.
[Graphical view]
PfamiPF00563. EAL. 1 hit.
PF01590. GAF. 2 hits.
PF00990. GGDEF. 2 hits.
PF00360. PHY. 1 hit.
[Graphical view]
PRINTSiPR01033. PHYTOCHROME.
SMARTiSM00052. EAL. 1 hit.
SM00065. GAF. 3 hits.
SM00267. GGDEF. 2 hits.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
SSF55073. SSF55073. 2 hits.
SSF55781. SSF55781. 4 hits.
TIGRFAMsiTIGR00254. GGDEF. 2 hits.
PROSITEiPS50883. EAL. 1 hit.
PS50887. GGDEF. 2 hits.
PS50046. PHYTOCHROME_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55434-1 [UniParc]FASTAAdd to Basket

« Hide

MNPNRSLEDF LRNVINKFHR ALTLRETLQV IVEEARIFLG VDRVKIYKFA     50
SDGSGEVLAE AVNRAALPSL LGLHFPVEDI PPQAREELGN QRKMIAVDVA 100
HRRKKSHELS GRISPTEHSN GHYTTVDSCH IQYLLAMGVL SSLTVPVMQD 150
QQLWGIMAVH HSKPRRFTEQ EWETMALLSK EVSLAITQSQ LSRQVHQQQV 200
QEALVQRLET TVAQYGDRPE TWQYALETVG QAVEADGAVL YIAPDLTGSV 250
AQHYQWNLRF DWGNWLETSL WQELMRGQPS AAMEPMAAVQ STWEKPRPFT 300
SVAPLPPTNC VPHGYTLGEL EQRSDWIAPP ESLSAENFQS FLIVPLAADQ 350
QWVGSLILLR KEKSLVKHWA GKRGIDRRNI LPRLSFEAWE ETQKLVPTWN 400
RSERKLAQVA STQLYMAITQ QFVTRLITQQ TAYDPLTQLP NWIIFNRQLT 450
LALLDALYEG KMVGVLVIAM DRFKRINESF GHKTGDGLLQ EVADRLNQKL 500
SPLAAYSPLL SRWHGDGFTI LLTQISDNQE MIPLCERLLS TFQEPFFLQG 550
QPIYLTASMG ISTAPYDGET AESLLKFAEI ALTRAKCQGK NTYQFYRPQD 600
SAPMLDRLTL ESDLRQALTN QEFVLYFQPQ VALDTGKLLG VEALVRWQHP 650
RLGQVAPDVF IPLAEELGLI NHLGQWVLET ACATHQHFFR ETGRRLRMAV 700
NISARQFQDE KWLNSVLECL KRTGMPPEDL ELEITESLMM EDIKGTVVLL 750
HRLREEGVQV AIDDFGTGYS SLSILKQLPI HRLKIDKSFV NDLLNEGADT 800
AIIQYVIDLA NGLNLETVAE GIESEAQLQR LQKMGCHLGQ GYFLTRPLPA 850
EAMMTYLYYP QILDFGPTPP LPKVALPETE TEAGQGNVGD RPLPNSLNRE 900
NPWTEKLHDY VLLKERLQQR NVKEKLVLKI ANKIRASLNI NDILYSTVTE 950
VRQFLNTDRV VLFKFNSQWS GQVVTESHND FCRSIINDEI DDPCFKGHYL 1000
RLYREGRVRA VSDIEKADLA DCHKELLRHY QVKANLVVPV VFNENLWGLL 1050
IAHECKTPRY WQEEDLQLLM ELATQVAIAI HQGELYEQLE TANIRLQQIS 1100
SLDALTQVGN RYLFDSTLER EWQRLQRIRE PLALLLCDVD FFKGFNDNYG 1150
HPAGDRCLKK IADAMAKVAK RPTDLVARYG GEEFAIILSE TSLEGAINVT 1200
EALQVEVANL AIPHTVSGTG HVTLSIGIAV YTPERHINPN ALVKAADLAL 1250
YEAKAKGRNQ WLAYEGSQLP HVDGEV 1276
Length:1,276
Mass (Da):144,687
Last modified:November 1, 1996 - v1
Checksum:i318CF3A73962D99E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA10536.1.
PIRiS75801.
RefSeqiNP_442466.1. NC_000911.1.
YP_005652527.1. NC_017277.1.
YP_007452342.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA10536; BAA10536; BAA10536.
GeneIDi952361.
KEGGisyn:sll0821.
syy:SYNGTS_2574.
syz:MYO_125990.
PATRICi23842638. VBISynSp132158_2858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA10536.1 .
PIRi S75801.
RefSeqi NP_442466.1. NC_000911.1.
YP_005652527.1. NC_017277.1.
YP_007452342.1. NC_020286.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4BWI X-ray 2.60 A/B 2-424 [» ]
ProteinModelPortali Q55434.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48801N.
IntActi Q55434. 11 interactions.
STRINGi 1148.sll0821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA10536 ; BAA10536 ; BAA10536 .
GeneIDi 952361.
KEGGi syn:sll0821.
syy:SYNGTS_2574.
syz:MYO_125990.
PATRICi 23842638. VBISynSp132158_2858.

Phylogenomic databases

eggNOGi COG2203.
KOi K02488.
OMAi AIHQGEL.
OrthoDBi EOG6G4VQG.

Family and domain databases

Gene3Di 3.20.20.450. 1 hit.
3.30.450.40. 4 hits.
InterProi IPR001054. A/G_cyclase.
IPR001633. Diguanylate_PEstase_EAL_dom.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR000160. GGDEF_dom.
IPR016132. Phyto_chromo_attachment.
IPR001294. Phytochrome.
IPR013515. Phytochrome_cen-reg.
[Graphical view ]
Pfami PF00563. EAL. 1 hit.
PF01590. GAF. 2 hits.
PF00990. GGDEF. 2 hits.
PF00360. PHY. 1 hit.
[Graphical view ]
PRINTSi PR01033. PHYTOCHROME.
SMARTi SM00052. EAL. 1 hit.
SM00065. GAF. 3 hits.
SM00267. GGDEF. 2 hits.
[Graphical view ]
SUPFAMi SSF141868. SSF141868. 1 hit.
SSF55073. SSF55073. 2 hits.
SSF55781. SSF55781. 4 hits.
TIGRFAMsi TIGR00254. GGDEF. 2 hits.
PROSITEi PS50883. EAL. 1 hit.
PS50887. GGDEF. 2 hits.
PS50046. PHYTOCHROME_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light."
    Park C.-M., Kim J.-I., Yang S.-S., Kang J.-G., Kang J.-H., Shim J.-Y., Chung Y.-H., Park Y.-M., Song P.-S.
    Biochemistry 39:10840-10847(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHROMOPHORE 1 BINDING, MUTAGENESIS OF CYS-129 AND HIS-130.
  4. "Defining the bilin lyase domain: lessons from the extended phytochrome superfamily."
    Wu S.-H., Lagarias J.C.
    Biochemistry 39:13487-13495(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF TWO BILIN LYASE DOMAINS.

Entry informationi

Entry nameiPHY2_SYNY3
AccessioniPrimary (citable) accession number: Q55434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

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