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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi207 – 2148ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Gene namesi
Name:dnaB
Ordered Locus Names:slr0833
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Replicative DNA helicase, 1st partPRO_0000013288Add
BLAST
Chaini381 – 809429Ssp dnaB inteinPRO_0000013289Add
BLAST
Chaini810 – 87263Replicative DNA helicase, 2nd partPRO_0000013290Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ55418.

Interactioni

Protein-protein interaction databases

IntActiQ55418. 1 interaction.

Structurei

Secondary structure

1
872
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi387 – 3904Combined sources
Turni391 – 3933Combined sources
Beta strandi396 – 3994Combined sources
Helixi400 – 4023Combined sources
Beta strandi409 – 4146Combined sources
Turni415 – 4184Combined sources
Beta strandi419 – 4246Combined sources
Beta strandi427 – 44115Combined sources
Beta strandi446 – 4494Combined sources
Beta strandi454 – 4574Combined sources
Beta strandi460 – 4634Combined sources
Helixi464 – 4663Combined sources
Beta strandi472 – 4765Combined sources
Beta strandi773 – 79321Combined sources
Turni795 – 7973Combined sources
Beta strandi799 – 8024Combined sources
Beta strandi805 – 8084Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MI8X-ray2.00A379-478[»]
A771-811[»]
ProteinModelPortaliQ55418.
SMRiQ55418. Positions 379-494.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ55418.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini176 – 443268SF4 helicase; first partPROSITE-ProRule annotationAdd
BLAST
Domaini494 – 643150DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST
Domaini606 – 870265SF4 helicase; second partPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000113195.
InParanoidiQ55418.
KOiK02314.
OMAiAGHETIN.
PhylomeDBiQ55418.

Family and domain databases

CDDicd00984. DnaB_C. 1 hit.
Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q55418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANPALPPQ NIEAEECILG GILLDPEAMG RIIDLLVVDA FYVKAHRLIY
60 70 80 90 100
EAMLSLHGQS QPTDLMSVSS WLQDHHHFEA IGGMVKLTQL LDRTISAVNI
110 120 130 140 150
DRFAALIMDK YLRRQLIAAG HDIVDLGYET SKELETIFDE SEQKIFRLTQ
160 170 180 190 200
SRPQAGLVPL SETLVNTFIE LDKLHEKLSS PGVETQFYDL DAMTGGLQRA
210 220 230 240 250
DLIILAGRPS MGKTAFGLGI AANIAKNQNL PVAIFSLEMS KEQLALRLVA
260 270 280 290 300
SESLIDSNRL RTGHFSQAEF EPLTAAMGTL SSLPIYIDDT ASISVTQMRS
310 320 330 340 350
QVRRLQSEQK GPLGMVLIDY LQLMEGGSDN RVQELSKITR SLKGLAREIN
360 370 380 390 400
APVIALSQLS RAVESRTNKR PMMSDLRESG CISGDSLISL ASTGKRVSIK
410 420 430 440 450
DLLDEKDFEI WAINEQTMKL ESAKVSRVFC TGKKLVYILK TRLGRTIKAT
460 470 480 490 500
ANHRFLTIDG WKRLDELSLK EHIALPRKLE SSSLQLMSDE ELGLLGHLIG
510 520 530 540 550
DGCTLPRHAI QYTSNKIELA EKVVELAKAV FGDQINPRIS QERQWYQVYI
560 570 580 590 600
PASYRLTHNK KNPITKWLEN LDVFGLRSYE KFVPNQVFEQ PQRAIAIFLR
610 620 630 640 650
HLWSTDGCVK LIVEKSSRPV AYYATSSEKL AKDVQSLLLK LGINARLSKI
660 670 680 690 700
SQNGKGRDNY HVTITGQADL QIFVDQIGAV DKDKQASVEE IKTHIAQHQA
710 720 730 740 750
NTNRDVIPKQ IWKTYVLPQI QIKGITTRDL QMRLGNAYCG TALYKHNLSR
760 770 780 790 800
ERAAKIATIT QSPEIEKLSQ SDIYWDSIVS ITETGVEEVF DLTVPGPHNF
810 820 830 840 850
VANDIIVHNS IEQDADLIMM IYRDEYYNPD TPDPGVAELL IVKHRNGPTG
860 870
VVKLLFKPEF TQFLNLQRSN DY
Length:872
Mass (Da):97,813
Last modified:November 1, 1996 - v1
Checksum:iAC97823A1CB85109
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10516.1.
PIRiS75781.

Genome annotation databases

EnsemblBacteriaiBAA10516; BAA10516; BAA10516.
KEGGisyn:slr0833.
PATRICi23842592. VBISynSp132158_2835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10516.1.
PIRiS75781.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MI8X-ray2.00A379-478[»]
A771-811[»]
ProteinModelPortaliQ55418.
SMRiQ55418. Positions 379-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55418. 1 interaction.

Protein family/group databases

MEROPSiN10.002.

Proteomic databases

PRIDEiQ55418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10516; BAA10516; BAA10516.
KEGGisyn:slr0833.
PATRICi23842592. VBISynSp132158_2835.

Phylogenomic databases

HOGENOMiHOG000113195.
InParanoidiQ55418.
KOiK02314.
OMAiAGHETIN.
PhylomeDBiQ55418.

Miscellaneous databases

EvolutionaryTraceiQ55418.

Family and domain databases

CDDicd00984. DnaB_C. 1 hit.
Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAB_SYNY3
AccessioniPrimary (citable) accession number: Q55418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.