Replicative DNA helicase - Synechocystis sp. (strain PCC 6803 / Kazusa)

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Q55418 (DNAB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

Ssp dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	slr0833

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis ›

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Replicative DNA helicase, 1st part

PRO_0000013288

Chain

381 – 809

429

Ssp dnaB intein

PRO_0000013289

Chain

810 – 872

Replicative DNA helicase, 2nd part

PRO_0000013290

Regions

Domain

176 – 443

268

SF4 helicase; first part

Domain

494 – 643

150

DOD-type homing endonuclease

Domain

606 – 870

265

SF4 helicase; second part

Nucleotide binding

207 – 214

ATP Potential

Secondary structure

872

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q55418 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AC97823A1CB85109
Show »

FASTA

872

97,813

        10         20         30         40         50         60 
MAANPALPPQ NIEAEECILG GILLDPEAMG RIIDLLVVDA FYVKAHRLIY EAMLSLHGQS 

        70         80         90        100        110        120 
QPTDLMSVSS WLQDHHHFEA IGGMVKLTQL LDRTISAVNI DRFAALIMDK YLRRQLIAAG 

       130        140        150        160        170        180 
HDIVDLGYET SKELETIFDE SEQKIFRLTQ SRPQAGLVPL SETLVNTFIE LDKLHEKLSS 

       190        200        210        220        230        240 
PGVETQFYDL DAMTGGLQRA DLIILAGRPS MGKTAFGLGI AANIAKNQNL PVAIFSLEMS 

       250        260        270        280        290        300 
KEQLALRLVA SESLIDSNRL RTGHFSQAEF EPLTAAMGTL SSLPIYIDDT ASISVTQMRS 

       310        320        330        340        350        360 
QVRRLQSEQK GPLGMVLIDY LQLMEGGSDN RVQELSKITR SLKGLAREIN APVIALSQLS 

       370        380        390        400        410        420 
RAVESRTNKR PMMSDLRESG CISGDSLISL ASTGKRVSIK DLLDEKDFEI WAINEQTMKL 

       430        440        450        460        470        480 
ESAKVSRVFC TGKKLVYILK TRLGRTIKAT ANHRFLTIDG WKRLDELSLK EHIALPRKLE 

       490        500        510        520        530        540 
SSSLQLMSDE ELGLLGHLIG DGCTLPRHAI QYTSNKIELA EKVVELAKAV FGDQINPRIS 

       550        560        570        580        590        600 
QERQWYQVYI PASYRLTHNK KNPITKWLEN LDVFGLRSYE KFVPNQVFEQ PQRAIAIFLR 

       610        620        630        640        650        660 
HLWSTDGCVK LIVEKSSRPV AYYATSSEKL AKDVQSLLLK LGINARLSKI SQNGKGRDNY 

       670        680        690        700        710        720 
HVTITGQADL QIFVDQIGAV DKDKQASVEE IKTHIAQHQA NTNRDVIPKQ IWKTYVLPQI 

       730        740        750        760        770        780 
QIKGITTRDL QMRLGNAYCG TALYKHNLSR ERAAKIATIT QSPEIEKLSQ SDIYWDSIVS 

       790        800        810        820        830        840 
ITETGVEEVF DLTVPGPHNF VANDIIVHNS IEQDADLIMM IYRDEYYNPD TPDPGVAELL 

       850        860        870 
IVKHRNGPTG VVKLLFKPEF TQFLNLQRSN DY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[3]	"Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein." Wu H., Xu M.Q., Liu X.Q. Biochim. Biophys. Acta 1387:422-432(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF INTEIN.
[4]	"Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation." Mathys S., Evans T.C. Jr., Chute I.C., Wu H., Chong S., Benner J., Liu X.Q., Xu M.Q. Gene 231:1-13(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF INTEIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000022 Genomic DNA. Translation: BAA10516.1.

PIR

S75781.

RefSeq

NP_442446.1. NC_000911.1.
YP_005652507.1. NC_017277.1.
YP_007452322.1. NC_020286.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MI8	X-ray	2.00	A	379-811	[»]

ProteinModelPortal

Q55418.

SMR

Q55418. Positions 379-494.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q55418. 1 interaction.

STRING

1148.slr0833.

Protein family/group databases

MEROPS

N10.002.

Proteomic databases

PRIDE

Q55418.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAA10516; BAA10516; BAA10516.

GeneID

12253819.
14618003.
952377.

KEGG

syn:slr0833.
syy:SYNGTS_2554.

PATRIC

23842592. VBISynSp132158_2835.

Phylogenomic databases

eggNOG

COG0305.

HOGENOM

HOG000113195.

K02314.

OMA

LPRHAIQ.

ProtClustDB

PRK07773.

Family and domain databases

Gene3D

1.10.860.10. 1 hit.

InterPro

IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view]

Pfam

PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 2 hits.
[Graphical view]

PRINTS

PR00379. INTEIN.

SMART

SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]

SUPFAM

SSF48024. DNA_helicase_DnaB-like_N. 1 hit.

TIGRFAMs

TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.

PROSITE

PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q55418.

Entry information

Entry name

DNAB_SYNY3

Accession

Primary (citable) accession number: Q55418

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents