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Protein

Citrate synthase, mitochondrial

Gene

cs

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathway: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (cs), Citrate synthase (gltA), Citrate synthase, peroxisomal (cshA)
  2. Probable aconitate hydratase, mitochondrial (aco2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei292 – 2921PROSITE-ProRule annotation
Active sitei339 – 3391PROSITE-ProRule annotation
Active sitei394 – 3941PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiREACT_350347. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:cs
ORF Names:DDB_G0275311
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275311.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
BLAST
Chaini26 – 460435Citrate synthase, mitochondrialPRO_0000327624Add
BLAST

Proteomic databases

PRIDEiQ553V1.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0220638.

Structurei

3D structure databases

ProteinModelPortaliQ553V1.
SMRiQ553V1. Positions 19-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
InParanoidiQ553V1.
KOiK01647.
OMAiITDYLWS.
PhylomeDBiQ553V1.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q553V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFARLARANY LKNSGRYFST EPTLKERLVQ IIPGKIEQVK QLKTEHGDKI
60 70 80 90 100
IGTCTVAQAY GGMRSVKSLV TETSSLDPEE GIRFRGLTIP ECQEKLPKAP
110 120 130 140 150
GGAEPLPEGI LWLLLTGEVP TESQVKTLSK DLAKRAGLPK HVTSMIKAFP
160 170 180 190 200
EQMHPMSQLA AAILALQGES KFVKAYNDGV KKDKYWESTL EDSLDVIAKL
210 220 230 240 250
PEVAALIYQN TYKKSDITHK IDENLDWSAN FNRMLGYTSK DFDELMRLYL
260 270 280 290 300
TIHTDHEGGN VSAHTTHLVG SALSDSYLSL SAGMCGLAGP LHGLANQEVL
310 320 330 340 350
SWTMKLQEKL GNKEVSNEVL SEAIWEGLNA GRVVPGFGHA VLRKTDPRYT
360 370 380 390 400
CQREFALKHL PQDPLFKLVS QIYEVVPDIL TKHGKTKNPY PNVDAHSGCL
410 420 430 440 450
LQYYGLKEHN FYTVLFGVSR AIGVLSSLVW DRILGHPIER PKSVTTEWIS
460
SYVNSDQSKK
Length:460
Mass (Da):51,209
Last modified:May 24, 2005 - v1
Checksum:iD18EFF2775762E52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69936.1.
RefSeqiXP_643860.1. XM_638768.1.

Genome annotation databases

EnsemblProtistsiDDB0220638; DDB0220638; DDB_G0275311.
GeneIDi8619911.
KEGGiddi:DDB_G0275311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69936.1.
RefSeqiXP_643860.1. XM_638768.1.

3D structure databases

ProteinModelPortaliQ553V1.
SMRiQ553V1. Positions 19-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0220638.

Proteomic databases

PRIDEiQ553V1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0220638; DDB0220638; DDB_G0275311.
GeneIDi8619911.
KEGGiddi:DDB_G0275311.

Organism-specific databases

dictyBaseiDDB_G0275311.

Phylogenomic databases

eggNOGiCOG0372.
InParanoidiQ553V1.
KOiK01647.
OMAiITDYLWS.
PhylomeDBiQ553V1.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
ReactomeiREACT_350347. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ553V1.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCISYM_DICDI
AccessioniPrimary (citable) accession number: Q553V1
Secondary accession number(s): Q86I73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.