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Protein

DNA mismatch repair protein Msh2

Gene

msh2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (msh2-msh6 heterodimer) and MutS beta (msh2-msh3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi707 – 7148ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DDI-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein Msh2
Alternative name(s):
MutS protein homolog 2
Gene namesi
Name:msh2
ORF Names:DDB_G0275809
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275809. msh2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937DNA mismatch repair protein Msh2PRO_0000328238Add
BLAST

Proteomic databases

PaxDbiQ553L4.
PRIDEiQ553L4.

Interactioni

Subunit structurei

Heterodimer consisting of msh2-msh6 (MutS alpha) or msh2-msh3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (mlh1-pms1). Interacts with exo1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi44689.DDB0229897.

Structurei

3D structure databases

ProteinModelPortaliQ553L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni635 – 70975Interaction with exo1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi97 – 1015Poly-Gln

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
InParanoidiQ553L4.
KOiK08735.
OMAiNDWYLAY.
PhylomeDBiQ553L4.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 3 hits.
PfamiPF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q553L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNEQEESS QVVLKEDKTF VTFFQSLVSS NEDTDTIRLF DRKGYYSIHG
60 70 80 90 100
EDAVFVAMMH FKSKKSLKYW SISDPNPKKK IKIDNDGSLT TTASSSQQQQ
110 120 130 140 150
QELGLAVLTI RQGYEFENIV KELLDEKKKI EIWSMKPNSK QQWELIKKGS
160 170 180 190 200
PGNTQMFEDV LLNGNCEGSV MMALKVTREK GSIVFGISFG DATFKTIGVS
210 220 230 240 250
QFMDNDNLSN LSSFIMQMSV KECLLCCDQK NYDYQKVKEK LSDAGIPFTE
260 270 280 290 300
LPKSDFSSKN AEQDLTRLLG SVKNNLPDIE QEHAIQSASC LIKHLDLLSN
310 320 330 340 350
PNYFGKFKLE KYDLDRYMKL DSSSFKGLHI IDLKDSSVSA AAGGGGAGGA
360 370 380 390 400
SSSSNKDQSL YNLLNQCNTP MGSRLLLQWV KQPLLNAEEI EARLNFVEAF
410 420 430 440 450
YNDLELRQSL RSNDLKKIGD LDRLSKKLHG QKATLEDCVN LYGIVTRLPV
460 470 480 490 500
VLQSLNNHSS IHQELIKVNF IESLESIISD FAKFCAMVEK TIDLDLANDK
510 520 530 540 550
HEYVIRSSFD ETLRGIQLKK DQISNKIERF RVDIADDLNL DEAKVKLHYS
560 570 580 590 600
EKDMFLLRIS RKDEVAIRDK KKYIVHATAK DGVRFATREI DTLNEAYKKW
610 620 630 640 650
SAEYLDKQDG LAKRTLQIAA SFVPLIEDLS SLIATLDVFV TLSHVSSIAP
660 670 680 690 700
IPFIRPEIIP LGSDENGAGT VIIGGRHPCV EIQDNVNFIA NDIDLTRGQS
710 720 730 740 750
QFQIITGPNM GGKSTFIRQV GLIVLMAQIG CFVPAQKATI AVVDCILSRV
760 770 780 790 800
GAGDSQLRGV STFMAEMLET SYILKVATKN SLIIIDELGR GTSTYDGFGL
810 820 830 840 850
AWGIAEYICN QIGGFCLFAT HFHELTILSD LLPMVKNLHV SASTQNNTFT
860 870 880 890 900
LLYKVEQGPC DQSFGIHVAI LANFPSQVIE NAKQKAKELE SFESNTLKQN
910 920 930
HNKFLEEFKE INFNSNDVEK SLSLVNSLLN KYSIDIN
Length:937
Mass (Da):104,962
Last modified:May 24, 2005 - v1
Checksum:i2D24BB8DB9370EA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69655.1.
RefSeqiXP_643399.1. XM_638307.1.

Genome annotation databases

EnsemblProtistsiDDB0229897; DDB0229897; DDB_G0275809.
GeneIDi8619985.
KEGGiddi:DDB_G0275809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69655.1.
RefSeqiXP_643399.1. XM_638307.1.

3D structure databases

ProteinModelPortaliQ553L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0229897.

Proteomic databases

PaxDbiQ553L4.
PRIDEiQ553L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0229897; DDB0229897; DDB_G0275809.
GeneIDi8619985.
KEGGiddi:DDB_G0275809.

Organism-specific databases

dictyBaseiDDB_G0275809. msh2.

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
InParanoidiQ553L4.
KOiK08735.
OMAiNDWYLAY.
PhylomeDBiQ553L4.

Enzyme and pathway databases

ReactomeiR-DDI-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DDI-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Miscellaneous databases

PROiQ553L4.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 3 hits.
PfamiPF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMSH2_DICDI
AccessioniPrimary (citable) accession number: Q553L4
Secondary accession number(s): Q86HA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 6, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.