ID   NMT_DICDI               Reviewed;         413 AA.
AC   Q553B6; Q869M9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   24-JAN-2024, entry version 101.
DE   RecName: Full=Glycylpeptide N-tetradecanoyltransferase;
DE            EC=2.3.1.97;
DE   AltName: Full=Myristoyl-CoA:protein N-myristoyltransferase;
DE            Short=NMT;
DE   AltName: Full=Peptide N-myristoyltransferase;
GN   Name=nmt; ORFNames=DDB_G0275863;
OS   Dictyostelium discoideum (Social amoeba).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Adds a myristoyl group to the N-terminal glycine residue of
CC       certain cellular proteins. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H(+) +
CC         N-tetradecanoylglycyl-[protein]; Xref=Rhea:RHEA:15521, Rhea:RHEA-
CC         COMP:12666, Rhea:RHEA-COMP:12667, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57385, ChEBI:CHEBI:64723,
CC         ChEBI:CHEBI:133050; EC=2.3.1.97;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NMT family. {ECO:0000305}.
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DR   EMBL; AAFI02000013; EAL69682.2; -; Genomic_DNA.
DR   RefSeq; XP_643504.2; XM_638412.2.
DR   AlphaFoldDB; Q553B6; -.
DR   SMR; Q553B6; -.
DR   STRING; 44689.Q553B6; -.
DR   PaxDb; 44689-DDB0302502; -.
DR   EnsemblProtists; EAL69682; EAL69682; DDB_G0275863.
DR   GeneID; 8620085; -.
DR   KEGG; ddi:DDB_G0275863; -.
DR   dictyBase; DDB_G0275863; nmt.
DR   eggNOG; KOG2779; Eukaryota.
DR   HOGENOM; CLU_022882_0_1_1; -.
DR   InParanoid; Q553B6; -.
DR   OMA; GWKRDWH; -.
DR   PhylomeDB; Q553B6; -.
DR   Reactome; R-DDI-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR   PRO; PR:Q553B6; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004379; F:glycylpeptide N-tetradecanoyltransferase activity; ISS:dictyBase.
DR   GO; GO:0006499; P:N-terminal protein myristoylation; ISS:dictyBase.
DR   Gene3D; 3.40.630.170; -; 1.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000903; NMT.
DR   InterPro; IPR022677; NMT_C.
DR   InterPro; IPR022678; NMT_CS.
DR   InterPro; IPR022676; NMT_N.
DR   PANTHER; PTHR11377:SF5; GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE; 1.
DR   PANTHER; PTHR11377; N-MYRISTOYL TRANSFERASE; 1.
DR   Pfam; PF01233; NMT; 1.
DR   Pfam; PF02799; NMT_C; 1.
DR   PIRSF; PIRSF015892; N-myristl_transf; 1.
DR   SUPFAM; SSF55729; Acyl-CoA N-acyltransferases (Nat); 2.
DR   PROSITE; PS00975; NMT_1; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; Reference proteome; Transferase.
FT   CHAIN           1..413
FT                   /note="Glycylpeptide N-tetradecanoyltransferase"
FT                   /id="PRO_0000331294"
FT   BINDING         34
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         35
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         163
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         164
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         165
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         166
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         174
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         175
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         176
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
SQ   SEQUENCE   413 AA;  47700 MW;  B25A64368D84FEA9 CRC64;
     MADDKKKQLE ESKRLVELFK ASSLASKKPK GHEFWDTQPV PKIDDKIIES GPIENKTLDD
     VRKDPLTLPP AFEWIELDCN KPEELKEIYT LLYENYVEDD DNMFRFDYSP EFLKWALQPP
     GFLKEWHIGV RVVESKKLVG FISGIPATIR VEGKPITMVE INFLCVHKKL REKRLAPVLI
     KEVTRRVNLR NIWQAAYTAG VVLPKPVAIC KYWHRSIKQQ KLVEVGFSAI PPKMTMASMV
     KLYRINDEMK HPLRPLTKED VPSFKVLLDD YLSKYKIAPE FTTDEDVWHW FETRKDVITC
     YVKVDESTKK VTDAFSFYNL PSTIIGNAKH NTLKAAFSFY NVATTMSITD LVGDALVTAK
     KMDYDVFNCL DVFENSTFFK DLKFAVGDGD LQYYLYNYST PTKKSSEIGL VLL
//