Q553B6 (NMT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase
Short name=NMT
Peptide N-myristoyltransferase

Gene names

Name:	nmt
ORF Names:	DDB_G0275863

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Glycylpeptide N-tetradecanoyltransferase		PRO_0000331294

Sequences

	Sequence		Length	Mass (Da)
	Q553B6 [UniParc]. Last modified January 19, 2010. Version 2. Checksum: B25A64368D84FEA9	FASTA	413	47,700
	10 20 30 40 50 60 MADDKKKQLE ESKRLVELFK ASSLASKKPK GHEFWDTQPV PKIDDKIIES GPIENKTLDD 70 80 90 100 110 120 VRKDPLTLPP AFEWIELDCN KPEELKEIYT LLYENYVEDD DNMFRFDYSP EFLKWALQPP 130 140 150 160 170 180 GFLKEWHIGV RVVESKKLVG FISGIPATIR VEGKPITMVE INFLCVHKKL REKRLAPVLI 190 200 210 220 230 240 KEVTRRVNLR NIWQAAYTAG VVLPKPVAIC KYWHRSIKQQ KLVEVGFSAI PPKMTMASMV 250 260 270 280 290 300 KLYRINDEMK HPLRPLTKED VPSFKVLLDD YLSKYKIAPE FTTDEDVWHW FETRKDVITC 310 320 330 340 350 360 YVKVDESTKK VTDAFSFYNL PSTIIGNAKH NTLKAAFSFY NVATTMSITD LVGDALVTAK 370 380 390 400 410 KMDYDVFNCL DVFENSTFFK DLKFAVGDGD LQYYLYNYST PTKKSSEIGL VLL

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69682.2.
RefSeq	XP_643504.2. XM_638412.2.
3D structure databases
HSSP	HSSP built from PDB template 1RXT based on UniProtKB P30419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q553B6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0302502; DDB0302502; DDB_G0275863.
GeneID	8620085.
GenomeReviews	Gene locus nmt in contig CM000151_GR.
KEGG	ddi:DDB_G0275863.
Organism-specific databases
dictyBase	DDB_G0275863. nmt.
Phylogenomic databases
eggNOG	KOG2779.
GeneTree	EPrGT00050000005374.
HOGENOM	HBG314670.
OMA	EHAKGSP.
ProtClustDB	CLSZ2428978.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_DICDI

Accession

Primary (citable) accession number: Q553B6
Secondary accession number(s): Q869M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 19, 2010
Last modified:	December 14, 2011
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)