Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30GTPUniRule annotation1
Metal bindingi37Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi41Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Metal bindingi44Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei79GTPUniRule annotation1
Binding sitei83S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei109GTPUniRule annotation1
Binding sitei133S-adenosyl-L-methionineUniRule annotation1
Binding sitei169GTPUniRule annotation1
Binding sitei203S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi265Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi268Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi282Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi270 – 272GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:slr0901
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530051 – 327GTP 3',8-cyclaseAdd BLAST327

Proteomic databases

PRIDEiQ55369.

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ55369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228680.
InParanoidiQ55369.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiQ55369.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSFLCLVEL ALPMSKTLVD SYGRRIRKLR VSLTDQCNLR CHYCMPVDAI
60 70 80 90 100
FLEQSSYLSC QEYGEIIGEL IALGLEEVRL TGGEPLLRRN FTEIVRAIGQ
110 120 130 140 150
LKLKKIGLTT NGIVLDRHLD TLGENNVLDL NVSLDSLNAK TFSEITHRNC
160 170 180 190 200
LNTILRNLEL ASRQGFKIKL NTVVMREIND REIFDLIEYA KRWEMEIRFL
210 220 230 240 250
EIMRIGYACR HQEKTFISAQ ELLAKIQQKY SLKPIQSALD ATAFRYSTSC
260 270 280 290 300
GGIIGFIASE SQPFCGHCSR WRLSVDGTLR ACLLKNEGIN IRNFSSLERQ
310 320
HVYQQLLGMK PYLRPPEVSH AMHQIGG
Length:327
Mass (Da):37,365
Last modified:November 1, 1996 - v1
Checksum:i44D1854617A7C41E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10451.1.
PIRiS75716.

Genome annotation databases

EnsemblBacteriaiBAA10451; BAA10451; BAA10451.
KEGGisyn:slr0901.
PATRICi23842448. VBISynSp132158_2764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10451.1.
PIRiS75716.

3D structure databases

ProteinModelPortaliQ55369.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ55369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10451; BAA10451; BAA10451.
KEGGisyn:slr0901.
PATRICi23842448. VBISynSp132158_2764.

Phylogenomic databases

HOGENOMiHOG000228680.
InParanoidiQ55369.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiQ55369.

Enzyme and pathway databases

UniPathwayiUPA00344.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_SYNY3
AccessioniPrimary (citable) accession number: Q55369
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.