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Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).By similarity

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.By similarity
  • S-adenosyl-L-methionineBy similarityNote: Binds 1 S-adenosyl-L-methionine per subunit.By similarity

Pathway:imolybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301GTPBy similarity
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Binding sitei43 – 431S-adenosyl-L-methionineBy similarity
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Binding sitei79 – 791GTPBy similarity
Binding sitei83 – 831S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei109 – 1091GTPBy similarity
Binding sitei133 – 1331S-adenosyl-L-methionineBy similarity
Binding sitei169 – 1691GTPBy similarity
Binding sitei203 – 2031S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Metal bindingi265 – 2651Iron-sulfur 2 (4Fe-4S-substrate)By similarity
Metal bindingi268 – 2681Iron-sulfur 2 (4Fe-4S-substrate)By similarity
Metal bindingi282 – 2821Iron-sulfur 2 (4Fe-4S-substrate)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthase (EC:4.1.99.18)
Alternative name(s):
Molybdenum cofactor biosynthesis protein A
Gene namesi
Name:moaA
Ordered Locus Names:slr0901
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Cyclic pyranopterin monophosphate synthasePRO_0000153005Add
BLAST

Proteomic databases

PRIDEiQ55369.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ55369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni270 – 2723GTP bindingBy similarity

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.Curated

Phylogenomic databases

eggNOGiCOG2896.
HOGENOMiHOG000228680.
InParanoidiQ55369.
KOiK03639.
OMAiGWIHQIR.
OrthoDBiEOG64XXNN.
PhylomeDBiQ55369.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSFLCLVEL ALPMSKTLVD SYGRRIRKLR VSLTDQCNLR CHYCMPVDAI
60 70 80 90 100
FLEQSSYLSC QEYGEIIGEL IALGLEEVRL TGGEPLLRRN FTEIVRAIGQ
110 120 130 140 150
LKLKKIGLTT NGIVLDRHLD TLGENNVLDL NVSLDSLNAK TFSEITHRNC
160 170 180 190 200
LNTILRNLEL ASRQGFKIKL NTVVMREIND REIFDLIEYA KRWEMEIRFL
210 220 230 240 250
EIMRIGYACR HQEKTFISAQ ELLAKIQQKY SLKPIQSALD ATAFRYSTSC
260 270 280 290 300
GGIIGFIASE SQPFCGHCSR WRLSVDGTLR ACLLKNEGIN IRNFSSLERQ
310 320
HVYQQLLGMK PYLRPPEVSH AMHQIGG
Length:327
Mass (Da):37,365
Last modified:November 1, 1996 - v1
Checksum:i44D1854617A7C41E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10451.1.
PIRiS75716.

Genome annotation databases

EnsemblBacteriaiBAA10451; BAA10451; BAA10451.
KEGGisyy:SYNGTS_2488.
syz:MYO_125130.
PATRICi23842448. VBISynSp132158_2764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10451.1.
PIRiS75716.

3D structure databases

ProteinModelPortaliQ55369.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ55369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10451; BAA10451; BAA10451.
KEGGisyy:SYNGTS_2488.
syz:MYO_125130.
PATRICi23842448. VBISynSp132158_2764.

Phylogenomic databases

eggNOGiCOG2896.
HOGENOMiHOG000228680.
InParanoidiQ55369.
KOiK03639.
OMAiGWIHQIR.
OrthoDBiEOG64XXNN.
PhylomeDBiQ55369.

Enzyme and pathway databases

UniPathwayiUPA00344.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiMOAA_SYNY3
AccessioniPrimary (citable) accession number: Q55369
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.