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Q55369 (MOAA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic pyranopterin monophosphate synthase

EC=4.1.99.18
Alternative name(s):
Molybdenum cofactor biosynthesis protein A
Gene names
Name:moaA
Ordered Locus Names:slr0901
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z) By similarity. HAMAP-Rule MF_01225_B

Catalytic activity

GTP = cyclic pyranopterin phosphate + diphosphate. HAMAP-Rule MF_01225_B

Cofactor

Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate By similarity.

Binds 1 S-adenosyl-L-methionine per subunit By similarity.

Pathway

Cofactor biosynthesis; molybdopterin biosynthesis. HAMAP-Rule MF_01225_B

Subunit structure

Monomer and homodimer By similarity. HAMAP-Rule MF_01225_B

Sequence similarities

Belongs to the radical SAM superfamily. MoaA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Cyclic pyranopterin monophosphate synthase HAMAP-Rule MF_01225_B
PRO_0000153005

Regions

Region270 – 2723GTP binding By similarity

Sites

Metal binding371Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding411Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding441Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding2651Iron-sulfur 2 (4Fe-4S-substrate) By similarity
Metal binding2681Iron-sulfur 2 (4Fe-4S-substrate) By similarity
Metal binding2821Iron-sulfur 2 (4Fe-4S-substrate) By similarity
Binding site301GTP By similarity
Binding site431S-adenosyl-L-methionine By similarity
Binding site791GTP By similarity
Binding site831S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1091GTP By similarity
Binding site1331S-adenosyl-L-methionine By similarity
Binding site1691GTP By similarity
Binding site2031S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55369 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 44D1854617A7C41E

FASTA32737,365
        10         20         30         40         50         60 
MFSFLCLVEL ALPMSKTLVD SYGRRIRKLR VSLTDQCNLR CHYCMPVDAI FLEQSSYLSC 

        70         80         90        100        110        120 
QEYGEIIGEL IALGLEEVRL TGGEPLLRRN FTEIVRAIGQ LKLKKIGLTT NGIVLDRHLD 

       130        140        150        160        170        180 
TLGENNVLDL NVSLDSLNAK TFSEITHRNC LNTILRNLEL ASRQGFKIKL NTVVMREIND 

       190        200        210        220        230        240 
REIFDLIEYA KRWEMEIRFL EIMRIGYACR HQEKTFISAQ ELLAKIQQKY SLKPIQSALD 

       250        260        270        280        290        300 
ATAFRYSTSC GGIIGFIASE SQPFCGHCSR WRLSVDGTLR ACLLKNEGIN IRNFSSLERQ 

       310        320 
HVYQQLLGMK PYLRPPEVSH AMHQIGG 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA10451.1.
PIRS75716.
RefSeqNP_442381.1. NC_000911.1.
YP_005652441.1. NC_017277.1.
YP_007452257.1. NC_020286.1.

3D structure databases

ProteinModelPortalQ55369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.slr0901.

Proteomic databases

PRIDEQ55369.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10451; BAA10451; BAA10451.
GeneID953273.
KEGGsyn:slr0901.
syy:SYNGTS_2488.
syz:MYO_125130.
PATRIC23842448. VBISynSp132158_2764.

Phylogenomic databases

eggNOGCOG2896.
HOGENOMHOG000228680.
KOK03639.
OMAMFHQITG.
OrthoDBEOG64XXNN.
PhylomeDBQ55369.

Enzyme and pathway databases

UniPathwayUPA00344.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01225_B. MoaA_B.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR02666. moaA. 1 hit.
PROSITEPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMOAA_SYNY3
AccessionPrimary (citable) accession number: Q55369
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways