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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BRENDAi2.1.3.2. 6160.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:Saci_1596
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001132581 – 299Aspartate carbamoyltransferaseAdd BLAST299

Interactioni

Subunit structurei

Heterooligomer of catalytic and regulatory chains.

Protein-protein interaction databases

IntActiQ55338. 1 interactor.
STRINGi330779.Saci_1596.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 26Combined sources15
Turni35 – 38Combined sources4
Beta strandi40 – 47Combined sources8
Helixi50 – 62Combined sources13
Beta strandi66 – 71Combined sources6
Helixi84 – 94Combined sources11
Beta strandi96 – 105Combined sources10
Helixi107 – 114Combined sources8
Beta strandi119 – 124Combined sources6
Turni125 – 127Combined sources3
Helixi130 – 144Combined sources15
Beta strandi151 – 156Combined sources6
Helixi162 – 170Combined sources9
Helixi171 – 173Combined sources3
Beta strandi177 – 182Combined sources6
Helixi185 – 187Combined sources3
Helixi191 – 194Combined sources4
Beta strandi201 – 205Combined sources5
Helixi207 – 209Combined sources3
Helixi211 – 213Combined sources3
Beta strandi215 – 220Combined sources6
Beta strandi227 – 229Combined sources3
Helixi230 – 236Combined sources7
Helixi237 – 240Combined sources4
Helixi244 – 248Combined sources5
Beta strandi255 – 257Combined sources3
Beta strandi263 – 266Combined sources4
Helixi268 – 272Combined sources5
Helixi278 – 297Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PG5X-ray2.60A1-299[»]
2BE9X-ray2.60A1-299[»]
ProteinModelPortaliQ55338.
SMRiQ55338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ55338.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
HOGENOMiHOG000022685.
KOiK00609.
OMAiKQSFYGV.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHIISAYNF SRDELEDIFA LTDKYSKNLN DTRKILSGKT ISIAFFEPST
60 70 80 90 100
RTYLSFQKAI INLGGDVIGF SGEESTSVAK GENLADTIRM LNNYSDGIVM
110 120 130 140 150
RHKYDGASRF ASEISDIPVI NAGDGKHEHP TQAVIDIYTI NKHFNTIDGL
160 170 180 190 200
VFALLGDLKY ARTVNSLLRI LTRFRPKLVY LISPQLLRAR KEILDELNYP
210 220 230 240 250
VKEVENPFEV INEVDVLYVT RIQKERFVDE MEYEKIKGSY IVSLDLANKM
260 270 280 290
KKDSIILHPL PRVNEIDRKV DKTTKAKYFE QASYGVPVRM SILTKIYGE
Length:299
Mass (Da):34,171
Last modified:November 1, 1996 - v1
Checksum:i6A283A584F94E32D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99872 Genomic DNA. Translation: CAA68165.1.
AJ459777 Genomic DNA. Translation: CAD31976.1.
CP000077 Genomic DNA. Translation: AAY80909.1.
RefSeqiWP_011278411.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY80909; AAY80909; Saci_1596.
GeneIDi3474279.
KEGGisai:Saci_1596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99872 Genomic DNA. Translation: CAA68165.1.
AJ459777 Genomic DNA. Translation: CAD31976.1.
CP000077 Genomic DNA. Translation: AAY80909.1.
RefSeqiWP_011278411.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PG5X-ray2.60A1-299[»]
2BE9X-ray2.60A1-299[»]
ProteinModelPortaliQ55338.
SMRiQ55338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ55338. 1 interactor.
STRINGi330779.Saci_1596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY80909; AAY80909; Saci_1596.
GeneIDi3474279.
KEGGisai:Saci_1596.

Phylogenomic databases

eggNOGiarCOG00911. Archaea.
COG0540. LUCA.
HOGENOMiHOG000022685.
KOiK00609.
OMAiKQSFYGV.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BRENDAi2.1.3.2. 6160.

Miscellaneous databases

EvolutionaryTraceiQ55338.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_SULAC
AccessioniPrimary (citable) accession number: Q55338
Secondary accession number(s): Q4J8H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.