Reviewed, UniProtKB/Swiss-Prot Q55309 (CYSH_SYNE7)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Synonyms:	par
Ordered Locus Names:	Synpcc7942_2506
ORF Names:	sea0019

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100649
Experimental info
	Sequence conflict	218	1	S → T in AAA27328. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q55309-1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 549FFECCB3BF89A6	FASTA	232	26,622
	10 20 30 40 50 60 MPALLPSLTE INAQLADQAA TQIIQWAATE FGSGLVLSTS FGIQSAVMLH LATQVQPDIP 70 80 90 100 110 120 VIWIDTGYLP TETYRFAAEL TERLKLNLKV YQSEISPARM EALYGRLWES ESVEDFNRYD 130 140 150 160 170 180 QMRKVEPMNR ALQELGATAW LSGVRRQQTA HRQSMEIVEL KRDRYAIRPI LGWHSRDVYQ 190 200 210 220 230 YLTAHDLPYH PLFDQGYVTV GDWHSSRPLQ ADDSDERSTR FRGLKQECGL HL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the Synechococcus PCC 7942 PAPS reductase gene." Niehaus A., Gisselmann G., Schwenn J.D. Plant Mol. Biol. 20:1179-1183(1992) [PubMed: 1463852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Synechococcus elongatus PCC7942 genome sequence, cosmid 7H1 and 2E8." Holtman C.K., Socias T., Mohler B.J., Chen Y., Min H., Golden S.S., Youderian P., Sandoval P., Gonzalez A., Salinas I. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84476 Genomic DNA. Translation: AAA27328.1. U30252 Genomic DNA. Translation: AAL03931.1. CP000100 Genomic DNA. Translation: ABB58536.1.
PIR	S28609.
RefSeq	YP_401523.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
ModBase	Search...
Genome annotation databases
GeneID	3774526.
GenomeReviews	Gene locus Synpcc7942_2506 in contig CP000100_GR.
KEGG	syf:Synpcc7942_2506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55309.
Enzyme and pathway databases
BioCyc	SELO1140:SYNPCC7942_2506-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_SYNE7

Accession

Primary (citable) accession number: Q55309
Secondary accession number(s): Q31K83, Q935X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 18, 2006
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)